##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062569_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1430985
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332902161797644	32.0	32.0	32.0	32.0	32.0
2	31.565111444215	32.0	32.0	32.0	32.0	32.0
3	31.624590055101905	32.0	32.0	32.0	32.0	32.0
4	31.69780745430595	32.0	32.0	32.0	32.0	32.0
5	31.682045583985857	32.0	32.0	32.0	32.0	32.0
6	35.324319961425175	36.0	36.0	36.0	36.0	36.0
7	35.339494823495706	36.0	36.0	36.0	36.0	36.0
8	35.28783530225684	36.0	36.0	36.0	36.0	36.0
9	35.38792440172329	36.0	36.0	36.0	36.0	36.0
10	35.31371677550778	36.0	36.0	36.0	36.0	36.0
11	35.387530966432216	36.0	36.0	36.0	36.0	36.0
12	35.343316666491965	36.0	36.0	36.0	36.0	36.0
13	35.35188908339361	36.0	36.0	36.0	36.0	36.0
14	35.32672110469362	36.0	36.0	36.0	36.0	36.0
15	35.292495728466754	36.0	36.0	36.0	36.0	36.0
16	35.32394609307575	36.0	36.0	36.0	36.0	36.0
17	35.341170592284335	36.0	36.0	36.0	36.0	36.0
18	35.33408735940628	36.0	36.0	36.0	36.0	36.0
19	35.27892465679235	36.0	36.0	36.0	36.0	36.0
20	35.25152954084075	36.0	36.0	36.0	36.0	36.0
21	35.20787080227955	36.0	36.0	36.0	36.0	36.0
22	35.17013036474876	36.0	36.0	36.0	36.0	36.0
23	35.16100168764872	36.0	36.0	36.0	36.0	36.0
24	35.146653528862984	36.0	36.0	36.0	36.0	36.0
25	35.09438673361356	36.0	36.0	36.0	36.0	36.0
26	35.0627008668854	36.0	36.0	36.0	36.0	36.0
27	34.97942256557546	36.0	36.0	36.0	36.0	36.0
28	34.90489627773876	36.0	36.0	36.0	36.0	36.0
29	34.86312784550502	36.0	36.0	36.0	36.0	36.0
30	34.800085255960056	36.0	36.0	36.0	36.0	36.0
31	34.78967284772377	36.0	36.0	36.0	36.0	36.0
32	34.74311680415937	36.0	36.0	36.0	32.0	36.0
33	34.792944021076394	36.0	36.0	36.0	36.0	36.0
34	34.74289737488513	36.0	36.0	36.0	36.0	36.0
35	34.71540372540593	36.0	36.0	36.0	36.0	36.0
36	34.616854124955886	36.0	36.0	36.0	32.0	36.0
37	34.485337023099476	36.0	36.0	36.0	32.0	36.0
38	34.44463289272774	36.0	36.0	36.0	32.0	36.0
39	34.136849093456604	36.0	36.0	36.0	32.0	36.0
40	34.25499568479054	36.0	36.0	36.0	32.0	36.0
41	34.23436374245712	36.0	36.0	36.0	32.0	36.0
42	34.20673172674766	36.0	36.0	36.0	32.0	36.0
43	34.212826130252935	36.0	36.0	36.0	32.0	36.0
44	34.10235956351744	36.0	36.0	36.0	32.0	36.0
45	34.04248542088142	36.0	36.0	36.0	32.0	36.0
46	33.87398959457996	36.0	36.0	36.0	32.0	36.0
47	33.886436265928715	36.0	36.0	36.0	32.0	36.0
48	33.84003046852343	36.0	36.0	36.0	32.0	36.0
49	33.776143705210046	36.0	36.0	36.0	27.0	36.0
50	33.42266410898787	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	4.0
21	28.0
22	79.0
23	281.0
24	825.0
25	2281.0
26	5010.0
27	10314.0
28	17369.0
29	25623.0
30	35218.0
31	48779.0
32	70144.0
33	107104.0
34	221628.0
35	886294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14081846183524	21.389516179197447	13.24746270680486	26.22220265216246
2	10.007917944101969	21.10471044295062	50.14141182604004	18.745959786907367
3	19.44741443288595	26.93653728698195	30.423233835226437	23.192814444905665
4	10.32004595445645	18.29624691295907	40.68388002085281	30.699827111731665
5	9.043421139983996	41.73516843293256	37.354549488638945	11.866860938444498
6	29.1569617723251	39.145577957468284	19.618876307758534	12.078583962448086
7	25.704899565613594	32.04005076227268	23.755891051192744	18.499158620920987
8	20.846130462583464	46.91963926945426	20.278759036607653	11.955471231354627
9	26.192867150948473	15.361726363309188	21.962634129637976	36.48277235610436
10	15.475494152629132	30.229806741510217	36.24056157122541	18.054137534635235
11	32.155403445878186	19.389231892717255	31.832129617012058	16.623235044392498
12	26.970583199684135	22.838813824044276	35.77402977669228	14.416573199579311
13	31.8609209740144	24.16831762736856	26.38308577658047	17.58767562203657
14	17.551896071587052	27.739284478872943	27.313633616005756	27.395185833534242
15	18.234782335244603	43.721911829963275	22.212741573112226	15.830564261679891
16	16.091782932735143	27.981774791489777	38.77503957064539	17.151402705129684
17	16.92449606390004	34.12796081021115	32.91096692138632	16.03657620450249
18	17.447352697617376	26.421660604408853	42.184229743847766	13.946756954126005
19	24.402561871717733	25.908377795714145	27.96339584272372	21.725664489844405
20	23.80409298490201	29.782073187349972	31.81221326568762	14.601620562060399
21	18.713333822506875	27.8670985370217	27.062687589317846	26.356880051153574
22	22.03104854348578	35.753344724088656	27.476598287193788	14.73900844523178
23	15.963270055241669	34.948095193171135	31.910537147489315	17.17809760409788
24	23.712897060416427	27.8559873094407	33.15247888692055	15.278636743222327
25	19.486088253895044	31.727446479173437	32.60320688197291	16.183258384958613
26	13.96869988155012	34.12467635929098	34.53963528618399	17.36698847297491
27	16.66125081674511	33.91097740367649	31.96406670929465	17.463705070283755
28	15.307567864093613	34.33383298916481	28.539851920180855	21.818747226560724
29	19.007886176305135	29.759012148974307	27.966959821381778	23.266141853338784
30	16.0486657791661	30.1542643703463	32.477000108317	21.320069742170602
31	22.44817381034742	27.10447698613193	30.658881819166517	19.78846738435413
32	17.616956152580215	33.02997585579164	31.376010230715206	17.97705776091294
33	15.376820861155077	33.62536993749061	35.29526864362659	15.702540557727717
34	17.671394179533678	32.91963228126081	34.41727201892403	14.991701520281483
35	16.162084158813684	34.476951190962865	33.07609793254297	16.28486671768048
36	14.863817580198255	32.132412289437	35.75732799435354	17.246442136011208
37	16.196046778966934	31.81801346624877	28.981645509911004	23.004294244873286
38	18.178038204453575	36.07934394839918	28.05494117688166	17.68767667026559
39	21.938944153852066	28.428250470829536	30.672019622847202	18.9607857524712
40	24.30304999703002	28.149281788418467	32.02423505487479	15.523433159676724
41	22.652857996415058	25.727313703497938	34.80679392166934	16.81303437841766
42	19.352473995185136	33.813631868957394	32.6442275775078	14.189666558349668
43	15.247329636579	34.3069284443932	33.764994042565085	16.680747876462718
44	14.66095032442688	33.849481301341385	32.22612396356356	19.263444410668175
45	15.569904646100413	34.64354972274343	30.745745063714853	19.04080056744131
46	17.17343951675177	28.060640832211142	32.85874115904941	21.907178491987676
47	23.246505029752235	28.25165882241952	30.812831720807694	17.68900442702055
48	15.918545617179774	31.741003574460947	29.898915781786673	22.441535026572605
49	20.79036467887504	28.66354294419578	30.77411712911037	19.77197524781881
50	15.931275262336966	36.66001856065477	27.864462495737197	19.54424368127107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20.0
1	25.5
2	31.0
3	103.5
4	176.0
5	196.0
6	216.0
7	220.0
8	224.0
9	334.0
10	444.0
11	637.5
12	831.0
13	1423.5
14	2016.0
15	2739.5
16	3463.0
17	4023.0
18	4583.0
19	5229.5
20	5876.0
21	6856.0
22	7836.0
23	9293.5
24	10751.0
25	14007.5
26	17264.0
27	27613.0
28	37962.0
29	52800.5
30	67639.0
31	87878.0
32	108117.0
33	140969.0
34	173821.0
35	204247.0
36	234673.0
37	226613.0
38	218553.0
39	186910.5
40	155268.0
41	127218.5
42	99169.0
43	81849.0
44	64529.0
45	58335.0
46	52141.0
47	49585.5
48	47030.0
49	41085.0
50	35140.0
51	28291.5
52	21443.0
53	17747.5
54	14052.0
55	12936.0
56	11820.0
57	10697.5
58	9575.0
59	8453.5
60	7332.0
61	6268.0
62	5204.0
63	4461.0
64	3718.0
65	3224.0
66	2730.0
67	2434.0
68	2138.0
69	2061.5
70	1985.0
71	1557.5
72	1130.0
73	987.0
74	844.0
75	636.0
76	428.0
77	356.0
78	284.0
79	235.0
80	186.0
81	149.0
82	112.0
83	111.0
84	110.0
85	89.5
86	69.0
87	47.5
88	26.0
89	19.5
90	13.0
91	9.0
92	5.0
93	5.5
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01593308105954989
2	0.004053152199359183
3	2.0964580341513014E-4
4	2.0964580341513014E-4
5	0.0
6	4.1929160683026027E-4
7	6.988193447171005E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.397638689434201E-4
47	0.0
48	0.0
49	0.0
50	6.988193447171005E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1430985.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.074643604903386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07340477038528	44.650888735650426
2	11.533858598306493	12.704463033821611
3	3.1221639291507355	5.158561970221847
4	1.3076483318437253	2.8807306334735836
5	0.6851797917653963	1.8868016418380558
6	0.4527945101235001	1.4962497762785152
7	0.304421070181703	1.1736117362256402
8	0.2144918921697417	0.9450451613911913
9	0.1733104658204204	0.8590510924253497
>10	0.9730413703257069	10.128275366336803
>50	0.09052008801679544	3.501084269574023
>100	0.058828733144590584	6.522588400514502
>500	0.005359640100759926	2.0299265933796073
>1k	0.004466366750633272	4.172865060946606
>5k	5.104419143580882E-4	1.889856527922226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	8749	0.6113970446929912	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	6314	0.44123453425437725	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	6187	0.43235952857647003	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	5640	0.39413411042044466	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	4228	0.2954608189463901	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2907	0.20314678350926108	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	2560	0.17889775224757773	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2559	0.178827870313106	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	2470	0.1726083781451238	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2449	0.1711408575212179	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2195	0.15339084616540355	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	2156	0.15066545072100684	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	2099	0.1466821804561194	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	1813	0.1266959471972103	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1759	0.12292232273573797	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC	1724	0.12047645502922813	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	1709	0.11942822601215247	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	1647	0.11509554607490645	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1630	0.11390755318888737	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACA	1585	0.11076286613766041	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	1546	0.10803747069326372	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	1538	0.10747841521749006	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	1444	0.1009095133771493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.988193447171005E-5	0.0
9	0.0	0.0	0.0	1.397638689434201E-4	0.0
10	0.0	0.0	0.0	2.795277378868402E-4	0.0
11	0.0	0.0	0.0	3.4940967235855025E-4	0.0
12	0.0	0.0	0.0	3.4940967235855025E-4	0.0
13	0.0	0.0	0.0	3.4940967235855025E-4	0.0
14	0.0	0.0	0.0	3.4940967235855025E-4	0.0
15	0.0	0.0	0.0	3.4940967235855025E-4	0.0
16	0.0	0.0	0.0	4.891735413019703E-4	0.0
17	0.0	0.0	0.0	5.590554757736804E-4	0.0
18	0.0	0.0	0.0	6.988193447171005E-4	0.0
19	0.0	0.0	0.0	9.084651481322306E-4	0.0
20	0.0	0.0	0.0	0.0012578748204907808	0.0
21	0.0	0.0	0.0	0.001677166427321041	0.0
22	6.988193447171005E-5	0.0	0.0	0.0026555135099249816	0.0
23	6.988193447171005E-5	0.0	0.0	0.003913388330415763	0.0
24	6.988193447171005E-5	0.0	0.0	0.005939964430095354	0.0
25	6.988193447171005E-5	0.0	0.0	0.007826776660831525	0.0
26	6.988193447171005E-5	0.0	0.0	0.009783470826039406	0.0
27	6.988193447171005E-5	0.0	0.0	0.012578748204907808	0.0
28	6.988193447171005E-5	0.0	0.0	0.01795965715922948	0.0
29	6.988193447171005E-5	0.0	0.0	0.026625017033721526	0.0
30	6.988193447171005E-5	0.0	0.0	0.041929160683026025	0.0
31	6.988193447171005E-5	0.0	0.0	0.08127268979059879	0.0
32	6.988193447171005E-5	0.0	0.0	0.13508177933381552	0.0
33	6.988193447171005E-5	0.0	0.0	0.1877727579254849	0.0
34	6.988193447171005E-5	0.0	0.0	0.24877968671928777	0.0
35	6.988193447171005E-5	0.0	0.0	0.3123722470885439	0.0
36	6.988193447171005E-5	0.0	0.0	0.40405734511542746	0.0
37	6.988193447171005E-5	0.0	0.0	0.5682798911239461	0.0
38	6.988193447171005E-5	0.0	0.0	0.7427051995653343	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCGC	20	7.857405E-4	43.999683	10
TAGGACC	3235	0.0	27.134272	4
TTGGACG	50	8.3428895E-5	26.39981	4
ATACCGT	60	9.755149E-6	25.666483	6
CCGAGCC	45	0.0013983973	24.44427	14
TAAGACG	120	2.3646862E-11	23.833164	4
GTCCTAG	330	0.0	22.672844	1
AGTACTC	195	0.0	22.56394	5
AGGACCT	6125	0.0	22.448818	5
CCTACAC	330	0.0	21.999844	3
GTCGTAG	60	2.873273E-4	21.999844	11
GAGTTCG	70	3.2160624E-5	21.999844	44
CGTTTTG	180	0.0	21.999844	30
GGACCTG	6175	0.0	21.910774	6
TGTAGGA	10040	0.0	21.606176	2
CTAGGAC	495	0.0	21.333181	3
GGCGAAG	155	1.8189894E-12	21.290173	19
CTGTAGG	9820	0.0	21.244078	1
GTCCTAC	8965	0.0	20.987312	1
ACTGTTC	1530	0.0	20.849524	8
>>END_MODULE