FastQCFastQC Report
Thu 2 Feb 2017
SRR4062568_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062568_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630367
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG98530.6043424578637816No Hit
TCCATGTACTCTGCGTTGATACCAC83520.5122772970748304No Hit
GAGTACATGGAAGCAGTGGTATCAA41160.252458495541188No Hit
CATGTACTCTGCGTTGATACCACTG34340.21062742315073846No Hit
GTATCAACGCAGAGTACTTTTTTTT29270.17953013033261836No Hit
GCTTCCATGTACTCTGCGTTGATAC25370.15560913585714137No Hit
CCCATGTACTCTGCGTTGATACCAC24340.14929153988028462No Hit
GTACATGGGAAGCAGTGGTATCAAC23490.14407798980229605No Hit
GCGTTGATACCACTGCTTCCATGTA22790.13978447797336427No Hit
CATGGAAGCAGTGGTATCAACGCAG22390.13733104264254614No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22290.1367176838098416No Hit
TATCAACGCAGAGTACTTTTTTTTT21810.13377356141285981No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19540.11985031591046678No Hit
GAATAGGACCGCGGTTCTATTTTGT18410.11291936110090552No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18150.11132462813587371No Hit
GTATCAACGCAGAGTACATGGAAGC17620.10807382632253965No Hit
GTATCTGATCGTCTTCGAACCTCCG16470.10102019974643746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3500.014.1122785
CGTCTTA1900.013.50181215
TCTGCGA500.001497045713.3030110
CAAGACG3750.013.1702474
ACGGTAT3200.012.47118959
GTCGCGT1650.012.0925318
ATAAGGC1103.842797E-812.0843653
TCGCGTA1501.4551915E-1112.0356879
CGGTTCT3950.012.027312
GTCCTAA1750.011.93419551
CGCATCG2750.011.74594813
TCCTATA658.0644566E-411.6852632
CGCGTAA1552.5465852E-1111.64779810
CGTTATT1552.5465852E-1111.63814452
CGCAAGA3850.011.5904142
GGACCGT752.0713481E-411.4015296
ACTAGAC752.0843846E-411.393833
CTTACAT1001.9421805E-611.393833
CGCCAGT2850.011.336246518
GCGTAAC1604.5474735E-1111.28207111
Produced by FastQC (version 0.11.5)