##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062568_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1630367
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.101631718502645	32.0	32.0	32.0	32.0	32.0
2	30.681904749053434	32.0	32.0	32.0	32.0	32.0
3	30.680976737139552	32.0	32.0	32.0	32.0	32.0
4	30.76367897534727	32.0	32.0	32.0	32.0	32.0
5	30.514378051076843	32.0	32.0	32.0	32.0	32.0
6	34.1621162597133	36.0	36.0	36.0	32.0	36.0
7	34.03075442523064	36.0	36.0	36.0	32.0	36.0
8	34.01348224050168	36.0	36.0	36.0	32.0	36.0
9	34.21039680022964	36.0	36.0	36.0	32.0	36.0
10	33.80966923398229	36.0	36.0	36.0	32.0	36.0
11	34.21637459541318	36.0	36.0	36.0	32.0	36.0
12	33.937603005948965	36.0	36.0	36.0	32.0	36.0
13	34.08315857717925	36.0	36.0	36.0	32.0	36.0
14	33.95790334323499	36.0	36.0	36.0	32.0	36.0
15	33.8922420534763	36.0	36.0	36.0	32.0	36.0
16	33.88524730934814	36.0	36.0	36.0	32.0	36.0
17	33.794124267726225	36.0	36.0	36.0	32.0	36.0
18	33.821940090789376	36.0	36.0	36.0	32.0	36.0
19	33.806621453942576	36.0	36.0	36.0	32.0	36.0
20	33.79892625402747	36.0	36.0	36.0	32.0	36.0
21	33.762209367584106	36.0	36.0	36.0	32.0	36.0
22	33.717869657567896	36.0	36.0	36.0	27.0	36.0
23	33.645660148911254	36.0	36.0	36.0	27.0	36.0
24	33.617256728086375	36.0	36.0	36.0	27.0	36.0
25	33.11432088603364	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	70.0
5	134.0
6	239.0
7	78.0
8	232.0
9	289.0
10	177.0
11	52.0
12	117.0
13	89.0
14	304.0
15	384.0
16	602.0
17	821.0
18	1145.0
19	1603.0
20	2620.0
21	4047.0
22	5966.0
23	8795.0
24	12139.0
25	16832.0
26	22676.0
27	29192.0
28	38493.0
29	50406.0
30	64685.0
31	86448.0
32	123360.0
33	172512.0
34	363782.0
35	622066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.08377473998669	17.19581279655013	11.081121699418171	25.639290764045015
2	16.487190056284255	20.194124257190104	36.893545711944334	26.425139974581302
3	18.89631635185483	24.270036055023475	28.369623489375485	28.464024103746215
4	12.209151818303392	15.565845022470379	35.90555692557599	36.31944623365024
5	14.22406070765055	36.90782676133421	33.86434529930472	15.003767231710519
6	34.15419431283983	35.29215406054351	17.139778519713488	13.413873106903173
7	29.996335892480232	30.767210104344205	21.070705000064443	18.165749003111113
8	28.41176893077414	32.48506293120753	19.957016600972008	19.146151537046325
9	27.134494666564347	14.612723505486915	18.531931547847442	39.720850280101295
10	15.408865664126544	26.941720447274538	32.14794937765961	25.501464510939314
11	37.21789344473189	21.306386770213454	21.807090789293508	19.668628995761146
12	23.89281018295892	24.302504039516325	29.02347187753525	22.781213899989496
13	29.556796654559296	19.530913701999097	25.345827563655792	25.56646207978581
14	23.522953086735132	19.843323598116495	25.055819882931768	31.57790343221661
15	25.489354516847033	26.73064701819019	23.11244147670581	24.667556988256965
16	26.049483156139956	25.789756452489353	23.993344119462005	24.167416271908685
17	23.907060201958668	25.88427537317649	25.826429025847858	24.38223539901698
18	25.188950908690057	24.427190261451432	26.83892054020891	23.544938289649597
19	25.020557985802554	26.406648320570696	25.146331712236105	23.42646198139065
20	24.774665790588713	24.344323199076435	26.26299532690194	24.618015683432915
21	26.205794100271074	24.932784555616557	24.153036271161042	24.708385072951327
22	24.743138747152212	25.225504124951858	24.930912968460444	25.100444159435487
23	23.747628473913366	24.843766410284914	25.206197629118808	26.20240748668291
24	23.890617716584035	26.37005756412784	24.85790141813003	24.8814233011581
25	24.899313279101502	24.89912903990498	24.668768631188748	25.532789049804766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	252.0
1	252.0
2	590.0
3	928.0
4	928.0
5	928.0
6	2503.5
7	4079.0
8	4079.0
9	4079.0
10	4237.0
11	4395.0
12	4395.0
13	4395.0
14	4853.0
15	5311.0
16	5311.0
17	5311.0
18	8576.5
19	11842.0
20	11842.0
21	11842.0
22	18731.5
23	25621.0
24	25621.0
25	25621.0
26	37622.5
27	49624.0
28	49624.0
29	49624.0
30	60165.5
31	70707.0
32	70707.0
33	70707.0
34	87016.0
35	103325.0
36	103325.0
37	103325.0
38	115468.5
39	127612.0
40	127612.0
41	127612.0
42	147654.5
43	167697.0
44	167697.0
45	167697.0
46	205751.0
47	243805.0
48	243805.0
49	243805.0
50	231217.5
51	218630.0
52	218630.0
53	218630.0
54	198025.0
55	177420.0
56	177420.0
57	177420.0
58	162416.0
59	147412.0
60	147412.0
61	147412.0
62	130686.0
63	113960.0
64	113960.0
65	113960.0
66	94450.5
67	74941.0
68	74941.0
69	74941.0
70	56660.0
71	38379.0
72	38379.0
73	38379.0
74	29699.5
75	21020.0
76	21020.0
77	21020.0
78	17267.5
79	13515.0
80	13515.0
81	13515.0
82	9494.5
83	5474.0
84	5474.0
85	5474.0
86	4019.0
87	2564.0
88	2564.0
89	2564.0
90	1754.5
91	945.0
92	945.0
93	945.0
94	563.5
95	182.0
96	182.0
97	182.0
98	454.5
99	727.0
100	727.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009936413089813521
2	0.004354847712202222
3	0.0049682065449067605
4	0.013555230202770297
5	0.03207866695044735
6	0.042689774756235865
7	0.06428000566743562
8	0.08378481654743994
9	0.09151313783951712
10	0.11267401756782369
11	0.11408474288304411
12	0.12745596543600304
13	0.11543413231499412
14	0.1174582164629191
15	0.10684710865713058
16	0.10623374982442603
17	0.1174582164629191
18	0.1247571865721031
19	0.12549321717134854
20	0.11635417056405091
21	0.1239598200895872
22	0.1440166539190256
23	0.13469359966191663
24	0.12856001133487124
25	0.1256772248211599
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1630367.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.78337658398574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35224156180948	39.00639147870981
2	13.122230110131685	13.065378463888047
3	3.884203385025658	5.801062795365736
4	1.5637321492897336	3.1139146585830493
5	0.8028655988934622	1.998468022802024
6	0.4994996526391685	1.4920067586543477
7	0.3081946733979373	1.0740080040863604
8	0.21691377021093752	0.8638959926930602
9	0.17525178264196672	0.7852162943051861
>10	0.8612079715713332	8.001572582064425
>50	0.09084340348025874	3.2053808679731026
>100	0.10269648476967236	10.975590787726695
>500	0.01394784382621365	4.685343525731982
>1k	0.005924747819984559	4.81309545614323
>5k	2.4686449249935663E-4	1.1186743112730924
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	9853	0.6043424578637816	No Hit
TCCATGTACTCTGCGTTGATACCAC	8352	0.5122772970748304	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4116	0.252458495541188	No Hit
CATGTACTCTGCGTTGATACCACTG	3434	0.21062742315073846	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2927	0.17953013033261836	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2537	0.15560913585714137	No Hit
CCCATGTACTCTGCGTTGATACCAC	2434	0.14929153988028462	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2349	0.14407798980229605	No Hit
GCGTTGATACCACTGCTTCCATGTA	2279	0.13978447797336427	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2239	0.13733104264254614	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2229	0.1367176838098416	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2181	0.13377356141285981	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1954	0.11985031591046678	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1841	0.11291936110090552	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1815	0.11132462813587371	No Hit
GTATCAACGCAGAGTACATGGAAGC	1762	0.10807382632253965	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1647	0.10102019974643746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.453435330818153E-4
2	0.0	0.0	0.0	0.0	2.453435330818153E-4
3	0.0	0.0	0.0	0.0	2.453435330818153E-4
4	0.0	0.0	0.0	1.2267176654090766E-4	3.0667941635226914E-4
5	0.0	0.0	0.0	1.2267176654090766E-4	3.0667941635226914E-4
6	0.0	0.0	0.0	1.2267176654090766E-4	3.0667941635226914E-4
7	0.0	0.0	0.0	1.2267176654090766E-4	3.0667941635226914E-4
8	0.0	0.0	0.0	1.840076498113615E-4	3.0667941635226914E-4
9	0.0	0.0	0.0	2.453435330818153E-4	3.0667941635226914E-4
10	0.0	0.0	0.0	2.453435330818153E-4	3.68015299622723E-4
11	0.0	0.0	0.0	2.453435330818153E-4	4.293511828931768E-4
12	0.0	0.0	0.0	3.0667941635226914E-4	7.36030599245446E-4
13	0.0	0.0	0.0	3.68015299622723E-4	9.200382490568075E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	350	0.0	14.112278	5
CGTCTTA	190	0.0	13.501812	15
TCTGCGA	50	0.0014970457	13.30301	10
CAAGACG	375	0.0	13.170247	4
ACGGTAT	320	0.0	12.4711895	9
GTCGCGT	165	0.0	12.092531	8
ATAAGGC	110	3.842797E-8	12.084365	3
TCGCGTA	150	1.4551915E-11	12.035687	9
CGGTTCT	395	0.0	12.0273	12
GTCCTAA	175	0.0	11.9341955	1
CGCATCG	275	0.0	11.745948	13
TCCTATA	65	8.0644566E-4	11.685263	2
CGCGTAA	155	2.5465852E-11	11.647798	10
CGTTATT	155	2.5465852E-11	11.6381445	2
CGCAAGA	385	0.0	11.590414	2
GGACCGT	75	2.0713481E-4	11.401529	6
ACTAGAC	75	2.0843846E-4	11.39383	3
CTTACAT	100	1.9421805E-6	11.39383	3
CGCCAGT	285	0.0	11.3362465	18
GCGTAAC	160	4.5474735E-11	11.282071	11
>>END_MODULE