FastQCFastQC Report
Thu 2 Feb 2017
SRR4062568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630367
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA45950.2818383836277354No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39250.2407433418365313No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18890.11586348349788729No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18800.1153114605484532No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18400.11285802521763505No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA16740.1026762685947397No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16580.10169489446241245No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC16560.10157222269587154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA651.813982E-523.69216244
GTATTAC1452.9467628E-1019.7282491
TGGTATA3750.019.35987944
TTAACGG2700.018.74062235
CGTCTTA3750.017.5998915
TAACGGC2650.017.43385336
GTATAGG1651.6552804E-917.3369461
CGTCGTA4450.017.3032610
ATACCGT4450.017.303266
TACCGTC4450.017.303267
ACCGTCG4600.017.2172818
TACGACG5400.017.1110025
GGCGTTA5050.016.98999442
CGCAATA6050.016.72716736
ATAATAC2251.8189894E-1216.6226273
CCGTCGT4500.016.6221189
GTCTTAC2007.2759576E-1116.5034391
GTATTAG5900.016.41021
GTATAAG2950.016.41021
ACGACGG5950.016.2688056
Produced by FastQC (version 0.11.5)