FastQCFastQC Report
Thu 2 Feb 2017
SRR4062567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295082
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG60460.4668430261558727No Hit
TCCATGTACTCTGCGTTGATACCAC50260.3880835344788979No Hit
GTATCAACGCAGAGTACTTTTTTTT37310.2880898661243072No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27630.21334556421909964No Hit
GAGTACATGGAAGCAGTGGTATCAA25580.19751645069578605No Hit
TATCAACGCAGAGTACTTTTTTTTT24660.1904126534072746No Hit
CATGTACTCTGCGTTGATACCACTG21170.1634645528236822No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19490.15049240125335694No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15380.11875695901881116No Hit
GCGCAAGACGGACCAGAGCGAAAGC14910.11512784518663682No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14900.11505062999871823No Hit
GAATAACGCCGCCGCATCGCCAGTC14830.114510123683288No Hit
GAATAGGACCGCGGTTCTATTTTGT14480.11180759210613692No Hit
GCTTCCATGTACTCTGCGTTGATAC14470.1117303769182183No Hit
GATTAAGAGGGACGGCCGGGGGCAT14380.11103544022695087No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14320.11057214909943926No Hit
GCGTTGATACCACTGCTTCCATGTA14230.10987721240817185No Hit
GAACTACGACGGTATCTGATCGTCT13960.10779240233436956No Hit
GTATCTGATCGTCTTCGAACCTCCG13500.10424050369011384No Hit
GTCCTATTCCATTATTCCTAGCTGC13280.10254176955590458No Hit
CATGGAAGCAGTGGTATCAACGCAG13180.10176961767671854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG250.006031614318.999969
GTCCTAA1250.014.4327151
GGACCGA551.9604279E-413.817626
AAGACGG3000.013.2989455
CGGCGTC1450.013.10494114
CGAGCCG2500.012.92147315
CAAGACG3050.012.7689854
TCGGCGT1501.8189894E-1212.66762113
TATACTG1207.403287E-1012.6656615
GGGTAGG2350.012.5255931
GCCGCCT2450.012.41245718
GTCGGCG1551.8189894E-1212.25851312
GATCACG701.08986096E-412.21520613
TAGGACT701.09182976E-412.2128454
CTAGGAC701.09380184E-412.21048453
CGTTATT1103.831883E-812.0862122
GACGGAC3150.012.0630017
CTTACAC951.0432286E-611.9962643
GCGTTAT1052.7361602E-711.7559711
TTACACA1302.6175258E-911.6909284
Produced by FastQC (version 0.11.5)