##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062567_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1295082
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081404111863186	32.0	32.0	32.0	32.0	32.0
2	30.73535498138342	32.0	32.0	32.0	32.0	32.0
3	30.722003703240414	32.0	32.0	32.0	32.0	32.0
4	30.769148208375995	32.0	32.0	32.0	32.0	32.0
5	30.587419947153926	32.0	32.0	32.0	32.0	32.0
6	34.22108484250418	36.0	36.0	36.0	32.0	36.0
7	34.06870066914682	36.0	36.0	36.0	32.0	36.0
8	34.07252745386006	36.0	36.0	36.0	32.0	36.0
9	34.20493142519161	36.0	36.0	36.0	32.0	36.0
10	33.88382048395391	36.0	36.0	36.0	32.0	36.0
11	34.24386486724393	36.0	36.0	36.0	32.0	36.0
12	33.98646031679847	36.0	36.0	36.0	32.0	36.0
13	34.12129038933442	36.0	36.0	36.0	32.0	36.0
14	34.02289044245847	36.0	36.0	36.0	32.0	36.0
15	33.96291122878706	36.0	36.0	36.0	32.0	36.0
16	33.96774026663949	36.0	36.0	36.0	32.0	36.0
17	33.88117740807146	36.0	36.0	36.0	32.0	36.0
18	33.91453128064478	36.0	36.0	36.0	32.0	36.0
19	33.88451619279706	36.0	36.0	36.0	32.0	36.0
20	33.874774724689246	36.0	36.0	36.0	32.0	36.0
21	33.830391434673636	36.0	36.0	36.0	32.0	36.0
22	33.78273499284215	36.0	36.0	36.0	32.0	36.0
23	33.748750272183536	36.0	36.0	36.0	32.0	36.0
24	33.73266403208446	36.0	36.0	36.0	27.0	36.0
25	33.26106532250468	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	11.0
4	40.0
5	134.0
6	223.0
7	69.0
8	202.0
9	246.0
10	156.0
11	60.0
12	88.0
13	75.0
14	266.0
15	297.0
16	487.0
17	676.0
18	942.0
19	1382.0
20	2186.0
21	3099.0
22	4698.0
23	6840.0
24	9305.0
25	12876.0
26	17179.0
27	21942.0
28	29350.0
29	38857.0
30	49168.0
31	65814.0
32	93537.0
33	131910.0
34	286710.0
35	516255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53326939382566	17.503517445137895	11.261924949844552	25.701288211191887
2	16.644247831344465	20.490377918245123	36.63803953848601	26.2273347119244
3	19.11033515131345	24.706589129029744	28.14041714960383	28.042658570052982
4	12.77768124336326	15.669697847282556	35.63008012356405	35.92254078579013
5	14.549941681278531	36.62446605541437	33.315824843002915	15.509767420304184
6	33.66752803303321	35.18044273458714	17.2888976441823	13.86313158819735
7	29.712531902908523	30.60793827043158	21.27154194879926	18.407987877860638
8	28.486214814408346	31.92916857628437	20.122668946099704	19.461947663207585
9	27.634581138235514	14.453559768494472	18.82636467067349	39.085494422596526
10	16.228951439392592	26.48355581806529	31.353145774593987	25.93434696794813
11	37.25037707740466	21.393644071400022	21.97872286144348	19.377255989751845
12	24.665872311997358	23.951880973607683	28.629011368653508	22.75323534574145
13	29.932066929575203	19.637582055835466	25.216567103138125	25.21378391145121
14	24.00208126842951	19.849440485155114	24.867060138761538	31.281418107653842
15	25.4106591650684	26.808937291616903	23.102819979421625	24.67758356389307
16	25.827114225947245	25.662843210466345	24.11745880082932	24.392583762757095
17	23.89881286022985	25.58454360254978	25.715202424532933	24.801441112687435
18	25.019426461275813	24.26873387410416	27.03908256072074	23.672757103899286
19	25.356698540731248	25.676650540199287	25.393735033661457	23.57291588540801
20	25.49713751154853	23.962720001855494	25.86452563096513	24.67561685563085
21	26.725990231637155	24.377918273271383	24.143180663682706	24.75291083140876
22	25.630589385310685	24.884848222378267	24.737447760224455	24.74711463208659
23	24.01484965153117	24.36490366362029	25.613483768149337	26.006762916699206
24	24.334110668147108	25.72480801170332	25.25577820596486	24.685303114184713
25	24.851778197709482	24.691185248644587	25.206056913460735	25.250979640185196
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	224.0
1	224.0
2	615.5
3	1007.0
4	1007.0
5	1007.0
6	2637.0
7	4267.0
8	4267.0
9	4267.0
10	4296.5
11	4326.0
12	4326.0
13	4326.0
14	4318.5
15	4311.0
16	4311.0
17	4311.0
18	6966.0
19	9621.0
20	9621.0
21	9621.0
22	15369.0
23	21117.0
24	21117.0
25	21117.0
26	30673.0
27	40229.0
28	40229.0
29	40229.0
30	48926.0
31	57623.0
32	57623.0
33	57623.0
34	69755.0
35	81887.0
36	81887.0
37	81887.0
38	91482.5
39	101078.0
40	101078.0
41	101078.0
42	117162.5
43	133247.0
44	133247.0
45	133247.0
46	158087.5
47	182928.0
48	182928.0
49	182928.0
50	175802.0
51	168676.0
52	168676.0
53	168676.0
54	152186.0
55	135696.0
56	135696.0
57	135696.0
58	125329.5
59	114963.0
60	114963.0
61	114963.0
62	103375.5
63	91788.0
64	91788.0
65	91788.0
66	77468.0
67	63148.0
68	63148.0
69	63148.0
70	48506.0
71	33864.0
72	33864.0
73	33864.0
74	27173.0
75	20482.0
76	20482.0
77	20482.0
78	17147.0
79	13812.0
80	13812.0
81	13812.0
82	9915.5
83	6019.0
84	6019.0
85	6019.0
86	4472.5
87	2926.0
88	2926.0
89	2926.0
90	1995.5
91	1065.0
92	1065.0
93	1065.0
94	645.5
95	226.0
96	226.0
97	226.0
98	389.0
99	552.0
100	552.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008339240295209107
2	0.0030886075167441134
3	0.004246835335523156
4	0.015983543899150788
5	0.03644556869758054
6	0.04841392282496398
7	0.07111518807303321
8	0.0921177191868932
9	0.09960759241499766
10	0.12238607285098549
11	0.12207721209931109
12	0.13813797118638046
13	0.12377594623352035
14	0.12663290818650866
15	0.11512784518663682
16	0.11489619962288102
17	0.12640126262275286
18	0.13458607254212476
19	0.1361303763004968
20	0.1256291107435668
21	0.13304176878375268
22	0.1548936669647173
23	0.14307974321317105
24	0.13697974336760144
25	0.13512657885755497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1295082.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.0559933262128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71915569306502	40.70140681036446
2	12.641953304406888	12.908949670801839
3	3.420288483441209	5.238786779528906
4	1.400820087440628	2.8608104414237414
5	0.7159767851627815	1.8277452982497135
6	0.4117711215607866	1.2614030180600808
7	0.2714785318283988	0.9702424276468544
8	0.1975140485975726	0.8067420757624748
9	0.13917827848766848	0.639529673185817
>10	0.8502095145582897	8.378700138977557
>50	0.1037302938075079	3.7204913621896507
>100	0.1110997543418061	12.05624515016833
>500	0.011822342860581252	4.074387227585074
>1k	0.0046986234445899845	3.697755394634958
>5k	3.031369964251603E-4	0.8568045314205226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6046	0.4668430261558727	No Hit
TCCATGTACTCTGCGTTGATACCAC	5026	0.3880835344788979	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3731	0.2880898661243072	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2763	0.21334556421909964	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2558	0.19751645069578605	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2466	0.1904126534072746	No Hit
CATGTACTCTGCGTTGATACCACTG	2117	0.1634645528236822	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1949	0.15049240125335694	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1538	0.11875695901881116	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1491	0.11512784518663682	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1490	0.11505062999871823	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1483	0.114510123683288	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1448	0.11180759210613692	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1447	0.1117303769182183	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1438	0.11103544022695087	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1432	0.11057214909943926	No Hit
GCGTTGATACCACTGCTTCCATGTA	1423	0.10987721240817185	No Hit
GAACTACGACGGTATCTGATCGTCT	1396	0.10779240233436956	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1350	0.10424050369011384	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1328	0.10254176955590458	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1318	0.10176961767671854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.721518791860284E-5
7	0.0	0.0	0.0	7.721518791860284E-5	7.721518791860284E-5
8	0.0	0.0	0.0	7.721518791860284E-5	7.721518791860284E-5
9	0.0	0.0	0.0	2.3164556375580852E-4	1.5443037583720567E-4
10	0.0	0.0	0.0	2.3164556375580852E-4	1.5443037583720567E-4
11	0.0	0.0	0.0	3.0886075167441135E-4	1.5443037583720567E-4
12	0.0	0.0	0.0	3.0886075167441135E-4	1.5443037583720567E-4
13	0.0	0.0	0.0	3.0886075167441135E-4	1.5443037583720567E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	0.0060316143	18.99996	9
GTCCTAA	125	0.0	14.432715	1
GGACCGA	55	1.9604279E-4	13.81762	6
AAGACGG	300	0.0	13.298945	5
CGGCGTC	145	0.0	13.104941	14
CGAGCCG	250	0.0	12.921473	15
CAAGACG	305	0.0	12.768985	4
TCGGCGT	150	1.8189894E-12	12.667621	13
TATACTG	120	7.403287E-10	12.665661	5
GGGTAGG	235	0.0	12.525593	1
GCCGCCT	245	0.0	12.412457	18
GTCGGCG	155	1.8189894E-12	12.258513	12
GATCACG	70	1.08986096E-4	12.215206	13
TAGGACT	70	1.09182976E-4	12.212845	4
CTAGGAC	70	1.09380184E-4	12.2104845	3
CGTTATT	110	3.831883E-8	12.086212	2
GACGGAC	315	0.0	12.063001	7
CTTACAC	95	1.0432286E-6	11.996264	3
GCGTTAT	105	2.7361602E-7	11.755971	1
TTACACA	130	2.6175258E-9	11.690928	4
>>END_MODULE