FastQCFastQC Report
Thu 2 Feb 2017
SRR4062567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295082
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA26800.2069367036218556No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24080.18593417250799563No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15900.12277214879057852No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14920.11520506037455544No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14780.114124047743695No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14410.11126708579070668No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG14170.10941392128066023No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13820.10671138970350912No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13360.1031594910592534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT4200.021.9998846
TGGTATA2550.021.56851244
TACCGTC4200.020.952277
GTATTAT1201.42918E-820.1696741
TAACGGC1950.019.17938636
GTATTAG3800.019.1081141
GACCCGT708.124036E-418.8570446
GTCGTAG4550.018.85704411
CGTCGTA4550.018.85704410
TATTAGA3400.018.7660562
ACCGTCG4700.018.7233078
CCGTCGT4650.018.4515159
GTATAGA1203.1574564E-718.3360671
TTAACGG2050.018.24380735
AGATACC5200.016.9229894
TCCTACA5750.016.4533582
CCTACAG5750.016.069483
TATACTG1653.1110176E-815.9999165
ATTACAC1805.2659743E-915.8888053
TAGGACG3100.015.6128214
Produced by FastQC (version 0.11.5)