##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062567_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1295082
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257658588413708	32.0	32.0	32.0	32.0	32.0
2	31.438086545871226	32.0	32.0	32.0	32.0	32.0
3	31.516779632486593	32.0	32.0	32.0	32.0	32.0
4	31.613676199653767	32.0	32.0	32.0	32.0	32.0
5	31.56135518832012	32.0	32.0	32.0	32.0	32.0
6	35.149259274702295	36.0	36.0	36.0	36.0	36.0
7	35.159601476972114	36.0	36.0	36.0	36.0	36.0
8	35.11195121235566	36.0	36.0	36.0	36.0	36.0
9	35.20826094409466	36.0	36.0	36.0	36.0	36.0
10	35.099521883556406	36.0	36.0	36.0	36.0	36.0
11	35.22919784229879	36.0	36.0	36.0	36.0	36.0
12	35.14245584449479	36.0	36.0	36.0	36.0	36.0
13	35.176210463893405	36.0	36.0	36.0	36.0	36.0
14	35.13587402187661	36.0	36.0	36.0	36.0	36.0
15	35.10892900990053	36.0	36.0	36.0	36.0	36.0
16	35.11554249074576	36.0	36.0	36.0	36.0	36.0
17	35.08697904843091	36.0	36.0	36.0	36.0	36.0
18	35.09981838987802	36.0	36.0	36.0	36.0	36.0
19	35.074682529754874	36.0	36.0	36.0	36.0	36.0
20	35.058156163084654	36.0	36.0	36.0	36.0	36.0
21	35.04611213807311	36.0	36.0	36.0	36.0	36.0
22	35.02750559424037	36.0	36.0	36.0	36.0	36.0
23	34.98970875975421	36.0	36.0	36.0	36.0	36.0
24	34.96257457056773	36.0	36.0	36.0	36.0	36.0
25	34.9401759888563	36.0	36.0	36.0	32.0	36.0
26	34.89278593942314	36.0	36.0	36.0	32.0	36.0
27	34.87371996522228	36.0	36.0	36.0	32.0	36.0
28	34.85233676323198	36.0	36.0	36.0	32.0	36.0
29	34.83168556122315	36.0	36.0	36.0	32.0	36.0
30	34.80721220741235	36.0	36.0	36.0	32.0	36.0
31	34.79668082793213	36.0	36.0	36.0	32.0	36.0
32	34.75501087961998	36.0	36.0	36.0	32.0	36.0
33	34.72864420940141	36.0	36.0	36.0	32.0	36.0
34	34.70217484298291	36.0	36.0	36.0	32.0	36.0
35	34.658679527628365	36.0	36.0	36.0	32.0	36.0
36	34.6281393765028	36.0	36.0	36.0	32.0	36.0
37	34.59613368111054	36.0	36.0	36.0	32.0	36.0
38	34.55762183398426	36.0	36.0	36.0	32.0	36.0
39	34.50505218974551	36.0	36.0	36.0	32.0	36.0
40	34.47367425383103	36.0	36.0	36.0	32.0	36.0
41	34.44361052041492	36.0	36.0	36.0	32.0	36.0
42	34.37071011719721	36.0	36.0	36.0	32.0	36.0
43	34.381170458704545	36.0	36.0	36.0	32.0	36.0
44	34.346662991223724	36.0	36.0	36.0	32.0	36.0
45	34.25747481626646	36.0	36.0	36.0	32.0	36.0
46	34.286575676289225	36.0	36.0	36.0	32.0	36.0
47	34.2309112473187	36.0	36.0	36.0	32.0	36.0
48	34.19066900783116	36.0	36.0	36.0	32.0	36.0
49	34.185924134533565	36.0	36.0	36.0	32.0	36.0
50	33.650209793665574	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	10.0
21	31.0
22	105.0
23	382.0
24	994.0
25	2320.0
26	4744.0
27	8960.0
28	15188.0
29	22505.0
30	31727.0
31	44446.0
32	64766.0
33	100724.0
34	214330.0
35	783847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.176432656181824	18.070098889090534	11.767994841240728	25.985473613486914
2	16.098080035582353	21.05165618552243	36.34990548437712	26.500358294518094
3	18.51823253479528	24.894002277860356	28.62845781132367	27.959307376020693
4	12.550518192726615	16.042045343987002	35.767652604707976	35.6397838585784
5	14.197335919529358	36.891437678415485	33.34231604046388	15.568910361591282
6	33.78177617649648	35.30740328351894	17.08736334757705	13.823457192407536
7	29.630347445449356	30.66711657417567	21.268090567308146	18.43444541306683
8	28.444144849515318	32.103681465729586	20.0571083529846	19.395065331770496
9	27.76719929703293	14.204274323942423	18.74190205716704	39.28662432185761
10	16.24808313295992	26.50272337967789	31.353613130288277	25.895580357073918
11	37.4511420898445	21.284212119387036	21.965636152768706	19.299009637999756
12	24.60153102274605	23.98133863338383	28.590390415433152	22.826739928436965
13	30.027365062598356	19.38603115478402	25.309671511147556	25.27693227147007
14	24.008286733967424	19.711724817424688	24.96513734265475	31.314851105953135
15	25.536915809192006	26.72711071576935	22.978699418260774	24.757274056777874
16	25.972409469052927	25.55591074541998	24.03237787259803	24.439301912929064
17	24.08982597240947	25.526105682883397	25.60841707320463	24.7756512715025
18	25.20072088099441	23.95562597580694	27.100832225295385	23.742820917903266
19	25.45838796307879	25.544560112795946	25.422946191824146	23.57410573230112
20	25.548034796252285	23.762047499695	26.057114530199634	24.632803173853084
21	26.828725903070232	24.208351285864524	24.19090065339492	24.772022157670325
22	25.607953782077118	24.697895577268465	24.93216645741351	24.761984183240905
23	24.063572808517144	24.147042426657155	25.699144919008987	26.090239845816715
24	24.40216140754022	25.38565125605946	25.363490497126822	24.848696839273497
25	25.040499366063308	24.320622169098172	25.328357586623856	25.31052087821466
26	24.00473483532317	25.1637348059814	25.989242380019178	24.842287978676254
27	25.633666439654014	24.895334812776333	25.07014999822405	24.400848749345602
28	24.344250016601265	24.279852549877152	25.992099341972168	25.383798091549416
29	24.099632301275133	25.219561386846546	26.050242378474874	24.630563933403447
30	24.732179120704327	24.742371525509583	26.214093007238155	24.311356346547942
31	24.51744368310269	24.877961395494648	25.061887973116757	25.542706948285897
32	24.327185460071256	25.108448731431675	25.104665187223667	25.459700621273402
33	24.087277871208155	24.495669000109647	25.675594286693816	25.741458841988386
34	24.59102975718912	25.06196518830468	26.226833513244724	24.120171541261477
35	25.900676559476544	24.513274062955087	25.552822137903235	24.033227239665134
36	24.37598545883581	25.35090442149609	25.36874112990529	24.90436898976281
37	25.629805680258084	25.0209639235199	25.07130822604283	24.277922170179185
38	24.34957786456765	24.876339876548357	25.442790495119226	25.331291763764764
39	24.71642722236893	24.740595576187456	25.23523606999402	25.30774113144959
40	25.363953788254335	24.778199372703813	25.56525378315813	24.29259305588372
41	23.85424243406981	25.14852341396143	26.133403135863208	24.863831016105546
42	25.75365884167952	25.328512016999692	25.441323406548776	23.476505734772008
43	24.57496899810205	24.090752554664494	25.573361377889587	25.76091706934387
44	24.15105761642892	24.868232281816905	25.868555041302404	25.11215506045177
45	24.346025965923392	25.08790949144533	25.595522136822225	24.970542405809056
46	23.97641537479123	24.699227307017864	25.983162443121316	25.34119487506959
47	24.48485887380104	24.26108925921293	26.459946165570987	24.794105701415045
48	24.841284181233313	26.01418288571689	24.870934813394054	24.27359811965574
49	23.7666031957822	25.27924872710763	25.993334784978867	24.96081329213131
50	23.588776618005657	26.28065249922399	24.887613293984472	25.242957588785885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	22.5
2	28.0
3	77.5
4	127.0
5	147.0
6	167.0
7	191.0
8	215.0
9	292.5
10	370.0
11	595.5
12	821.0
13	1420.5
14	2020.0
15	2873.0
16	3726.0
17	4654.0
18	5582.0
19	6191.5
20	6801.0
21	7562.5
22	8324.0
23	8582.5
24	8841.0
25	9511.0
26	10181.0
27	11921.5
28	13662.0
29	15791.5
30	17921.0
31	19637.0
32	21353.0
33	24801.5
34	28250.0
35	32355.5
36	36461.0
37	43131.0
38	49801.0
39	52614.5
40	55428.0
41	60816.0
42	66204.0
43	67892.5
44	69581.0
45	78542.0
46	87503.0
47	94187.0
48	100871.0
49	100747.5
50	100624.0
51	93415.0
52	86206.0
53	83506.5
54	80807.0
55	82484.0
56	84161.0
57	83030.0
58	81899.0
59	74700.5
60	67502.0
61	59465.5
62	51429.0
63	45179.5
64	38930.0
65	33665.5
66	28401.0
67	25608.0
68	22815.0
69	21143.0
70	19471.0
71	15684.5
72	11898.0
73	10825.5
74	9753.0
75	7315.5
76	4878.0
77	4411.0
78	3944.0
79	3549.0
80	3154.0
81	2524.5
82	1895.0
83	1645.0
84	1395.0
85	1179.5
86	964.0
87	720.0
88	476.0
89	316.0
90	156.0
91	98.5
92	41.0
93	28.0
94	15.0
95	13.0
96	11.0
97	5.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01521139201996476
2	0.004478480899278965
3	5.405063154302199E-4
4	3.0886075167441135E-4
5	7.721518791860284E-5
6	6.177215033488227E-4
7	7.721518791860284E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.721518791860284E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1295082.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.00224291241089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2649379974361	44.14751617084579
2	12.468514259124365	13.715925000744345
3	3.3498632345440935	5.52749974049246
4	1.307773208394164	2.87721838729755
5	0.6893307682426905	1.8957369180941634
6	0.3651037525534011	1.2048915171704955
7	0.256423630018173	0.9872712350719207
8	0.1788052183153377	0.786775044142949
9	0.13911972674100617	0.6886707303705341
>10	0.7846524287985377	8.249881930548751
>50	0.08819187271865436	3.368467623416491
>100	0.09530082311208768	10.99497211563169
>500	0.008740599530470137	3.1834350182899303
>1k	0.0032424804709808575	2.37173856788294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2680	0.2069367036218556	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2408	0.18593417250799563	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1590	0.12277214879057852	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1492	0.11520506037455544	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1478	0.114124047743695	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1441	0.11126708579070668	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1417	0.10941392128066023	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1382	0.10671138970350912	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1336	0.1031594910592534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5443037583720567E-4
2	0.0	0.0	0.0	0.0	1.5443037583720567E-4
3	0.0	0.0	0.0	0.0	1.5443037583720567E-4
4	0.0	0.0	0.0	0.0	1.5443037583720567E-4
5	0.0	0.0	0.0	0.0	2.3164556375580852E-4
6	0.0	0.0	0.0	0.0	2.3164556375580852E-4
7	0.0	0.0	0.0	0.0	2.3164556375580852E-4
8	0.0	0.0	0.0	0.0	2.3164556375580852E-4
9	0.0	0.0	0.0	7.721518791860284E-5	2.3164556375580852E-4
10	0.0	0.0	0.0	1.5443037583720567E-4	2.3164556375580852E-4
11	0.0	0.0	0.0	1.5443037583720567E-4	2.3164556375580852E-4
12	0.0	0.0	0.0	1.5443037583720567E-4	3.0886075167441135E-4
13	0.0	0.0	0.0	1.5443037583720567E-4	3.0886075167441135E-4
14	0.0	0.0	0.0	1.5443037583720567E-4	3.0886075167441135E-4
15	0.0	0.0	0.0	1.5443037583720567E-4	4.6329112751161705E-4
16	0.0	0.0	0.0	2.3164556375580852E-4	4.6329112751161705E-4
17	0.0	0.0	0.0	3.0886075167441135E-4	4.6329112751161705E-4
18	0.0	0.0	0.0	3.8607593959301417E-4	4.6329112751161705E-4
19	0.0	0.0	0.0	5.405063154302199E-4	4.6329112751161705E-4
20	0.0	0.0	0.0	6.949366912674255E-4	5.405063154302199E-4
21	0.0	0.0	0.0	0.001389873382534851	5.405063154302199E-4
22	0.0	0.0	0.0	0.002007594885883674	5.405063154302199E-4
23	0.0	0.0	0.0	0.0027025315771510993	5.405063154302199E-4
24	0.0	0.0	0.0	0.004787341650953376	5.405063154302199E-4
25	0.0	0.0	0.0	0.006331645409325432	5.405063154302199E-4
26	0.0	0.0	0.0	0.007567088416023078	6.949366912674255E-4
27	0.0	0.0	0.0	0.010501265556929986	6.949366912674255E-4
28	0.0	0.0	0.0	0.018994936227976298	6.949366912674255E-4
29	0.0	0.0	0.0	0.03513291050296429	6.949366912674255E-4
30	0.0	0.0	0.0	0.06848987168380072	6.949366912674255E-4
31	0.0	0.0	0.0	0.14501012291113613	6.949366912674255E-4
32	0.0	0.0	0.0	0.22917467774241324	6.949366912674255E-4
33	0.0	0.0	0.0	0.31055948580862064	6.949366912674255E-4
34	0.0	0.0	0.0	0.40707847070687414	7.721518791860283E-4
35	0.0	0.0	0.0	0.5115506199607438	7.721518791860283E-4
36	0.0	0.0	0.0	0.6585683377577636	7.721518791860283E-4
37	0.0	0.0	0.0	0.8864303573055605	8.493670671046312E-4
38	0.0	0.0	0.0	1.1637100971212633	0.001003797442941837
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	420	0.0	21.999884	6
TGGTATA	255	0.0	21.568512	44
TACCGTC	420	0.0	20.95227	7
GTATTAT	120	1.42918E-8	20.169674	1
TAACGGC	195	0.0	19.179386	36
GTATTAG	380	0.0	19.108114	1
GACCCGT	70	8.124036E-4	18.857044	6
GTCGTAG	455	0.0	18.857044	11
CGTCGTA	455	0.0	18.857044	10
TATTAGA	340	0.0	18.766056	2
ACCGTCG	470	0.0	18.723307	8
CCGTCGT	465	0.0	18.451515	9
GTATAGA	120	3.1574564E-7	18.336067	1
TTAACGG	205	0.0	18.243807	35
AGATACC	520	0.0	16.922989	4
TCCTACA	575	0.0	16.453358	2
CCTACAG	575	0.0	16.06948	3
TATACTG	165	3.1110176E-8	15.999916	5
ATTACAC	180	5.2659743E-9	15.888805	3
TAGGACG	310	0.0	15.612821	4
>>END_MODULE