FastQCFastQC Report
Thu 2 Feb 2017
SRR4062566_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062566_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399573
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38790.16165376089829314No Hit
TATCAACGCAGAGTACTTTTTTTTT24810.10339339540826639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA402.7610446E-416.62567510
CGCAAGA4050.013.3650562
TGTGCGA604.0920603E-412.66718110
AAGACGG4700.012.5319015
CGGTCCA5650.012.27483610
CAAGACG4800.012.2687724
ACGATCA1251.4115358E-912.16074859
AGAATTT6250.012.01149617
GCGCAAG4950.011.5098541
TAATCGT1251.8339051E-811.3997512
GAATTTC6350.011.2237418
ACGGTAT4100.011.1226359
CGTCTTA1803.6379788E-1211.08378315
ATCGCCA3700.011.04352616
AATTTCA6500.010.964272519
GGTCCAA6850.010.95665111
TAGGACG2350.010.9130594
GCAAGAC5750.010.9000133
AAGAATT7350.010.86007716
TATTCCG700.001491927210.8571315
Produced by FastQC (version 0.11.5)