##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062566_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2399573
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21267617196893	32.0	32.0	32.0	32.0	32.0
2	30.853530607320554	32.0	32.0	32.0	32.0	32.0
3	30.838598367292846	32.0	32.0	32.0	32.0	32.0
4	30.91792414733788	32.0	32.0	32.0	32.0	32.0
5	30.709795867848154	32.0	32.0	32.0	32.0	32.0
6	34.37326807727875	36.0	36.0	36.0	32.0	36.0
7	34.26299095714113	36.0	36.0	36.0	32.0	36.0
8	34.25889189451623	36.0	36.0	36.0	32.0	36.0
9	34.42847790002638	36.0	36.0	36.0	32.0	36.0
10	34.08400161195346	36.0	36.0	36.0	32.0	36.0
11	34.40887316201674	36.0	36.0	36.0	32.0	36.0
12	34.17703691448437	36.0	36.0	36.0	32.0	36.0
13	34.301813697687045	36.0	36.0	36.0	32.0	36.0
14	34.19703255537548	36.0	36.0	36.0	32.0	36.0
15	34.14461656303017	36.0	36.0	36.0	32.0	36.0
16	34.14363972256731	36.0	36.0	36.0	32.0	36.0
17	34.06417433435032	36.0	36.0	36.0	32.0	36.0
18	34.08132988660899	36.0	36.0	36.0	32.0	36.0
19	34.072438304648365	36.0	36.0	36.0	32.0	36.0
20	34.064549401080946	36.0	36.0	36.0	32.0	36.0
21	34.051419148323475	36.0	36.0	36.0	32.0	36.0
22	34.00296136020867	36.0	36.0	36.0	32.0	36.0
23	33.96315344438365	36.0	36.0	36.0	32.0	36.0
24	33.93527598451891	36.0	36.0	36.0	32.0	36.0
25	33.47667105772569	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	22.0
4	102.0
5	216.0
6	404.0
7	126.0
8	358.0
9	452.0
10	274.0
11	80.0
12	170.0
13	141.0
14	408.0
15	514.0
16	751.0
17	993.0
18	1345.0
19	1873.0
20	2803.0
21	4276.0
22	6449.0
23	9809.0
24	13839.0
25	19691.0
26	27674.0
27	36298.0
28	49214.0
29	65297.0
30	84161.0
31	115944.0
32	165695.0
33	239672.0
34	538360.0
35	1012162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53446841569154	17.31177032503722	10.861331123851567	26.29243013541967
2	17.160008435139858	19.91413994497155	36.417174344838855	26.50867727504974
3	19.212598293932405	23.512150355333503	28.254889899126294	29.020361451607794
4	12.938573640024709	14.978630358978293	35.59060152600994	36.49219447498706
5	14.866936922815244	36.14373046868486	33.69237324030563	15.296959368194265
6	34.56393313851315	34.98866560125681	16.71487138990957	13.73252987032047
7	30.36306396813541	30.241791036062015	20.87291489167383	18.52223010412874
8	28.235578737932315	32.68545495877852	19.586596396535434	19.49236990675373
9	27.39097679750313	14.307149632786341	18.609635572879167	39.69223799683136
10	15.825275612037723	26.66137042040902	31.812442785102306	25.70091118245095
11	37.459819014493526	21.189816143827063	22.105300051404956	19.245064790274448
12	24.478372924132888	23.716755663200836	28.72395828986441	23.080913122801864
13	29.39692265067963	19.452576307848428	25.27931142681657	25.871189614655382
14	23.392161804444015	19.521341315846133	25.097973271823253	31.9885236078866
15	25.210009003664048	27.02488566014214	22.221485128934304	25.543620207259504
16	25.851047984449522	25.78024639952871	23.596912433417515	24.771793182604256
17	24.216443973772563	25.808341407359002	25.15269714183664	24.8225174770318
18	24.898035487906554	24.94819359862328	25.98761820747765	24.166152705992516
19	25.354146836516385	25.303070072396306	25.309621585441118	24.033161505646195
20	25.402003990623456	24.578802295568174	25.10562801414326	24.91356569966511
21	26.294545073674453	24.40915014515396	24.539970230606393	24.7563345505652
22	25.598795959653298	24.58900144536596	24.840928356510805	24.971274238469938
23	24.456909582446162	24.482783948211203	25.386675703219357	25.673630766123278
24	24.569569531974416	25.152629738407445	25.228579464696317	25.04922126492183
25	24.756530426081145	24.787076270302347	25.205621103005427	25.25077220061108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	249.0
1	249.0
2	658.5
3	1068.0
4	1068.0
5	1068.0
6	2884.5
7	4701.0
8	4701.0
9	4701.0
10	5227.5
11	5754.0
12	5754.0
13	5754.0
14	6713.5
15	7673.0
16	7673.0
17	7673.0
18	12265.5
19	16858.0
20	16858.0
21	16858.0
22	26361.5
23	35865.0
24	35865.0
25	35865.0
26	52126.0
27	68387.0
28	68387.0
29	68387.0
30	84592.0
31	100797.0
32	100797.0
33	100797.0
34	124235.0
35	147673.0
36	147673.0
37	147673.0
38	169409.5
39	191146.0
40	191146.0
41	191146.0
42	218854.0
43	246562.0
44	246562.0
45	246562.0
46	279755.5
47	312949.0
48	312949.0
49	312949.0
50	316218.5
51	319488.0
52	319488.0
53	319488.0
54	300158.0
55	280828.0
56	280828.0
57	280828.0
58	257428.0
59	234028.0
60	234028.0
61	234028.0
62	206224.5
63	178421.0
64	178421.0
65	178421.0
66	147992.5
67	117564.0
68	117564.0
69	117564.0
70	89411.5
71	61259.0
72	61259.0
73	61259.0
74	47529.0
75	33799.0
76	33799.0
77	33799.0
78	26878.5
79	19958.0
80	19958.0
81	19958.0
82	14084.0
83	8210.0
84	8210.0
85	8210.0
86	5940.0
87	3670.0
88	3670.0
89	3670.0
90	2497.5
91	1325.0
92	1325.0
93	1325.0
94	810.0
95	295.0
96	295.0
97	295.0
98	670.5
99	1046.0
100	1046.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009043275616120035
2	0.003625645062684069
3	0.0057093491216979025
4	0.015627780442603746
5	0.03588138389621821
6	0.04675831908427041
7	0.06984576005814368
8	0.08993266718703703
9	0.09714228323122488
10	0.12114655399106423
11	0.121563294802867
12	0.1343989118063922
13	0.12356365069952027
14	0.12506391762201025
15	0.1154788789505466
16	0.11393693794687638
17	0.12498056945964969
18	0.13131502979905177
19	0.13252357815327978
20	0.12314690988771754
21	0.13135670388023202
22	0.15169365549620703
23	0.1408167203081548
24	0.1354824379170794
25	0.1323152077473784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2399573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.66010071712716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.773362274366	42.269865534692244
2	12.74722167544173	13.680343979354992
3	3.6676133600153302	5.904135068697112
4	1.5602348432554185	3.3488943532582747
5	0.8270595587806406	2.219004961161596
6	0.49386650190753517	1.5900555739904165
7	0.3359744706453816	1.2619896753250262
8	0.24225907488363724	1.0399717086315219
9	0.18168419337036867	0.877427290346759
>10	1.0054996118249397	9.66139532336331
>50	0.07721356898475783	2.9001155969222028
>100	0.0743095119513184	8.603831359390309
>500	0.009964621507529818	3.5938698079202096
>1k	0.003736733065323499	3.049099766945952
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3879	0.16165376089829314	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2481	0.10339339540826639	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.334816236055332E-5
2	0.0	0.0	0.0	0.0	8.334816236055332E-5
3	0.0	0.0	0.0	0.0	8.334816236055332E-5
4	0.0	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
5	0.0	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
6	8.334816236055332E-5	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
7	8.334816236055332E-5	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
8	8.334816236055332E-5	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
9	8.334816236055332E-5	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
10	8.334816236055332E-5	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
11	8.334816236055332E-5	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
12	8.334816236055332E-5	0.0	0.0	4.167408118027666E-5	1.2502224354082997E-4
13	8.334816236055332E-5	0.0	0.0	4.167408118027666E-5	1.2502224354082997E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGA	40	2.7610446E-4	16.625675	10
CGCAAGA	405	0.0	13.365056	2
TGTGCGA	60	4.0920603E-4	12.667181	10
AAGACGG	470	0.0	12.531901	5
CGGTCCA	565	0.0	12.274836	10
CAAGACG	480	0.0	12.268772	4
ACGATCA	125	1.4115358E-9	12.1607485	9
AGAATTT	625	0.0	12.011496	17
GCGCAAG	495	0.0	11.509854	1
TAATCGT	125	1.8339051E-8	11.39975	12
GAATTTC	635	0.0	11.22374	18
ACGGTAT	410	0.0	11.122635	9
CGTCTTA	180	3.6379788E-12	11.083783	15
ATCGCCA	370	0.0	11.043526	16
AATTTCA	650	0.0	10.9642725	19
GGTCCAA	685	0.0	10.956651	11
TAGGACG	235	0.0	10.913059	4
GCAAGAC	575	0.0	10.900013	3
AAGAATT	735	0.0	10.860077	16
TATTCCG	70	0.0014919272	10.857131	5
>>END_MODULE