FastQCFastQC Report
Thu 2 Feb 2017
SRR4062566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399573
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC3200.018.56247336
CGCAATA7150.018.46151236
TCTAGCG7200.018.33330728
TTAACGG3250.018.27689635
CTAGCGG7500.017.59997629
TAGCGGC7700.016.85711930
TACCGTC5750.016.8347597
ATACGAA7850.016.81526240
CAATACG8000.016.77497738
GTATCAA42500.016.7727891
TACGAAT8300.016.16865241
CGAATGC8050.016.124243
TAGGACG3450.015.9420074
CGTCGTA5150.015.80580310
GTATTAC2800.015.7152451
ATACCGT6300.015.7142636
AATACGA8450.015.6212839
CGGCGCA11850.015.59491533
ACCGTCG5800.015.5517018
TATTAGA5400.015.0740522