##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2399573
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.321821007320885	32.0	32.0	32.0	32.0	32.0
2	31.437776637760134	32.0	32.0	32.0	32.0	32.0
3	31.5150203807094	32.0	32.0	32.0	32.0	32.0
4	31.612236427064314	32.0	32.0	32.0	32.0	32.0
5	31.554839965277157	32.0	32.0	32.0	32.0	32.0
6	35.15143402597045	36.0	36.0	36.0	36.0	36.0
7	35.172149378243546	36.0	36.0	36.0	36.0	36.0
8	35.108920628795204	36.0	36.0	36.0	36.0	36.0
9	35.23257762943657	36.0	36.0	36.0	36.0	36.0
10	35.12099986122531	36.0	36.0	36.0	36.0	36.0
11	35.24704687042236	36.0	36.0	36.0	36.0	36.0
12	35.16030393740886	36.0	36.0	36.0	36.0	36.0
13	35.201269975949884	36.0	36.0	36.0	36.0	36.0
14	35.16545902125086	36.0	36.0	36.0	36.0	36.0
15	35.12567736009699	36.0	36.0	36.0	36.0	36.0
16	35.141606860887336	36.0	36.0	36.0	36.0	36.0
17	35.11310554002733	36.0	36.0	36.0	36.0	36.0
18	35.12614994417757	36.0	36.0	36.0	36.0	36.0
19	35.113962359136394	36.0	36.0	36.0	36.0	36.0
20	35.11117061243813	36.0	36.0	36.0	36.0	36.0
21	35.10347966075631	36.0	36.0	36.0	36.0	36.0
22	35.09031523525227	36.0	36.0	36.0	36.0	36.0
23	35.053280729529796	36.0	36.0	36.0	36.0	36.0
24	35.026134233048964	36.0	36.0	36.0	36.0	36.0
25	35.00664451550338	36.0	36.0	36.0	36.0	36.0
26	34.96538217424517	36.0	36.0	36.0	36.0	36.0
27	34.94538528313162	36.0	36.0	36.0	32.0	36.0
28	34.93838820490146	36.0	36.0	36.0	32.0	36.0
29	34.91842340283042	36.0	36.0	36.0	32.0	36.0
30	34.90456218668905	36.0	36.0	36.0	32.0	36.0
31	34.89807978336145	36.0	36.0	36.0	32.0	36.0
32	34.86861704144862	36.0	36.0	36.0	32.0	36.0
33	34.850907640651066	36.0	36.0	36.0	32.0	36.0
34	34.837337309596336	36.0	36.0	36.0	32.0	36.0
35	34.811346018645814	36.0	36.0	36.0	32.0	36.0
36	34.787045028427976	36.0	36.0	36.0	32.0	36.0
37	34.779124035818036	36.0	36.0	36.0	32.0	36.0
38	34.7461089952254	36.0	36.0	36.0	32.0	36.0
39	34.74272589331519	36.0	36.0	36.0	32.0	36.0
40	34.737808351735914	36.0	36.0	36.0	32.0	36.0
41	34.70023916755189	36.0	36.0	36.0	32.0	36.0
42	34.68011767093562	36.0	36.0	36.0	32.0	36.0
43	34.67030342481767	36.0	36.0	36.0	32.0	36.0
44	34.64769398555493	36.0	36.0	36.0	32.0	36.0
45	34.61050861965858	36.0	36.0	36.0	32.0	36.0
46	34.61413718190695	36.0	36.0	36.0	32.0	36.0
47	34.584746119413744	36.0	36.0	36.0	32.0	36.0
48	34.55151479033978	36.0	36.0	36.0	32.0	36.0
49	34.54092165564457	36.0	36.0	36.0	32.0	36.0
50	34.031538944637234	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	16.0
21	52.0
22	195.0
23	536.0
24	1350.0
25	3217.0
26	6992.0
27	13137.0
28	21948.0
29	34379.0
30	49341.0
31	71823.0
32	106614.0
33	177589.0
34	402269.0
35	1510109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05649902133718	17.899067686120993	11.345569675123958	26.69886361741786
2	16.533435911498824	20.422126377022266	36.149259565962666	26.89517814551624
3	18.604210190289134	23.887139261839362	28.550787873938503	28.957862673933
4	12.605654522526159	15.51943143051702	35.75529908325088	36.119614963705935
5	14.547094231804673	36.4653363183101	33.52789580808577	15.459673641799455
6	34.765910044678854	35.15889728530415	16.441702655768598	13.633490014248393
7	30.35584277356244	30.38226416305248	20.834140769329185	18.427752294055896
8	28.29815971424916	32.806253445925584	19.408619783603164	19.486967056222085
9	27.408293058806716	14.134306395346172	18.49312356823485	39.964276977612265
10	15.823190209258065	26.75271808775978	31.77177772878758	25.652313974194573
11	37.63007001662379	21.101754353795446	22.046047359259337	19.222128270321427
12	24.486523227257518	23.681796719666373	28.6807277794841	23.15095227359201
13	29.447489199120007	19.385407320385752	25.325089088767044	25.842014391727197
14	23.43604466294628	19.437708292267	25.025369096918492	32.10087794786822
15	25.27812239927687	27.021724281778464	22.20674261629048	25.493410702654177
16	25.953700929290335	25.72674388318255	23.50018107388273	24.819374113644386
17	24.253065024485608	25.772627046562036	25.13313827085069	24.84116965810167
18	25.05212385703623	24.865132254780328	25.971412413791956	24.111331474391484
19	25.53996065133255	25.190023391661764	25.269079123660752	24.000936833344934
20	25.487159590477138	24.479480307538047	25.093381197404707	24.93997890458011
21	26.403781006037324	24.3667519179454	24.541741384821382	24.68772569119589
22	25.6619823610284	24.54690897088774	24.830792811887783	24.96031585619608
23	24.529822597603822	24.302740529252496	25.40522834687671	25.762208526266967
24	24.80182932546749	25.022910326128855	25.116843705109197	25.05841664329445
25	24.955606685022712	24.54749240802426	25.2429911488419	25.25390975811113
26	24.656761848878947	25.05679135412842	25.578925917236106	24.707520879756522
27	25.39560163412407	24.91910018990879	24.774116061482605	24.911182114484536
28	24.68426674245793	24.60441920291652	25.58551042206259	25.12580363256296
29	24.668055524878802	25.032495364800322	25.374222830478587	24.92522627984229
30	24.757404754929315	24.94993900998219	25.648146566076548	24.644509669011946
31	24.9752768513398	24.769906979283398	24.919808649288854	25.33500752008795
32	24.725732453232304	24.932519244048837	24.937103392978667	25.404644909740192
33	24.55620229099094	24.565578959256502	25.322380273490324	25.55583847626223
34	25.06279242181838	24.697477426192076	25.59284506035032	24.64688509163922
35	25.668816910341963	24.5989598982819	25.281539673933658	24.450683517442478
36	24.627798362458652	24.92101719764308	25.19173202899016	25.259452410908107
37	25.609556366903615	24.669847510369554	24.897221297289143	24.823374825437693
38	24.654136381764587	24.646426676746238	25.492410524705853	25.207026416783318
39	24.994280232358008	24.647968617749907	24.982861534114612	25.374889615777473
40	25.27983103660526	24.617754908894206	25.450528073119678	24.651885981380854
41	24.407425821177352	24.98206972657219	25.6896539509321	24.92085050131836
42	25.44644401316401	25.009741316475893	25.244033000871408	24.299781669488695
43	24.73902648512881	24.216975270183486	25.508913460853243	25.535084783834456
44	24.449224924601168	24.63342436341799	25.483742315820358	25.433608396160484
45	24.57358038284311	24.826792100094476	25.46623920172464	25.13338831533777
46	24.39323529080823	24.588311827405814	25.58036415675969	25.438088725026265
47	24.5683294486144	24.46439429015079	25.862601387830253	25.10467487340456
48	24.996905699472364	25.255034958302996	25.0382463880032	24.709812954221437
49	24.16609121706237	25.032703735206223	25.51824845503762	25.282956592693782
50	24.087837330990695	25.321932411280013	25.311847279431497	25.2783829782978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	40.5
2	53.0
3	176.0
4	299.0
5	357.5
6	416.0
7	379.0
8	342.0
9	475.0
10	608.0
11	888.5
12	1169.0
13	1985.5
14	2802.0
15	3645.5
16	4489.0
17	5183.0
18	5877.0
19	6438.0
20	6999.0
21	8538.5
22	10078.0
23	10829.0
24	11580.0
25	14248.0
26	16916.0
27	21048.0
28	25180.0
29	28482.0
30	31784.0
31	34750.0
32	37716.0
33	43523.0
34	49330.0
35	56843.5
36	64357.0
37	76409.0
38	88461.0
39	96454.5
40	104448.0
41	114646.0
42	124844.0
43	128059.0
44	131274.0
45	143779.5
46	156285.0
47	165568.5
48	174852.0
49	180295.0
50	185738.0
51	180380.0
52	175022.0
53	172251.5
54	169481.0
55	168932.0
56	168383.0
57	163953.0
58	159523.0
59	146211.5
60	132900.0
61	116784.0
62	100668.0
63	88010.5
64	75353.0
65	64498.5
66	53644.0
67	47412.5
68	41181.0
69	36854.0
70	32527.0
71	25452.5
72	18378.0
73	16327.5
74	14277.0
75	10639.5
76	7002.0
77	6321.0
78	5640.0
79	4802.5
80	3965.0
81	3125.5
82	2286.0
83	1947.0
84	1608.0
85	1333.0
86	1058.0
87	776.0
88	494.0
89	328.0
90	162.0
91	105.0
92	48.0
93	34.5
94	21.0
95	18.5
96	16.0
97	11.0
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015877824929685407
2	0.004250756280388219
3	5.834371365238732E-4
4	2.917185682619366E-4
5	4.1674081180276654E-5
6	1.6669632472110662E-4
7	8.334816236055331E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.334816236055331E-5
47	0.0
48	0.0
49	0.0
50	4.1674081180276654E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2399573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.24243295586906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08910176716233	44.48163503679533
2	12.928955719930446	14.54311850535176
3	3.49880118039592	5.903432724429992
4	1.4475261141665232	3.256495617115214
5	0.7607322231524053	2.1392715529009187
6	0.4984322194927745	1.6819824412720428
7	0.3149261383476587	1.2398548555448259
8	0.23882771795778585	1.074580153219518
9	0.1762302422776555	0.892045582748784
>10	0.9007895816609265	9.023780698864108
>50	0.07025705440406019	2.763734594887975
>100	0.0639388511999578	7.585915277180214
>500	0.008946381702637568	3.381948243651171
>1k	0.0025348081490802242	2.032204716038194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.334816236055332E-5
2	0.0	0.0	0.0	0.0	8.334816236055332E-5
3	0.0	0.0	0.0	0.0	8.334816236055332E-5
4	0.0	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
5	0.0	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
6	0.0	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
7	4.167408118027666E-5	0.0	0.0	4.167408118027666E-5	8.334816236055332E-5
8	4.167408118027666E-5	0.0	0.0	4.167408118027666E-5	1.2502224354082997E-4
9	4.167408118027666E-5	0.0	0.0	4.167408118027666E-5	1.2502224354082997E-4
10	4.167408118027666E-5	0.0	0.0	4.167408118027666E-5	1.2502224354082997E-4
11	4.167408118027666E-5	0.0	0.0	4.167408118027666E-5	1.2502224354082997E-4
12	4.167408118027666E-5	0.0	0.0	4.167408118027666E-5	2.083704059013833E-4
13	4.167408118027666E-5	0.0	0.0	4.167408118027666E-5	3.333926494422133E-4
14	4.167408118027666E-5	0.0	0.0	8.334816236055332E-5	3.333926494422133E-4
15	4.167408118027666E-5	0.0	0.0	1.6669632472110664E-4	3.333926494422133E-4
16	4.167408118027666E-5	0.0	0.0	2.917185682619366E-4	3.333926494422133E-4
17	4.167408118027666E-5	0.0	0.0	2.917185682619366E-4	3.333926494422133E-4
18	4.167408118027666E-5	0.0	0.0	3.7506673062248993E-4	3.333926494422133E-4
19	4.167408118027666E-5	0.0	0.0	3.7506673062248993E-4	3.333926494422133E-4
20	4.167408118027666E-5	0.0	0.0	4.167408118027666E-4	3.333926494422133E-4
21	4.167408118027666E-5	0.0	0.0	7.918075424252565E-4	3.7506673062248993E-4
22	4.167408118027666E-5	0.0	0.0	0.0011252001918674699	4.5841489298304323E-4
23	4.167408118027666E-5	0.0	0.0	0.001458592841309683	5.000889741633199E-4
24	8.334816236055332E-5	0.0	0.0	0.002208726302554663	5.000889741633199E-4
25	8.334816236055332E-5	0.0	0.0	0.0026671411955377063	5.000889741633199E-4
26	8.334816236055332E-5	0.0	0.0	0.0037506673062248992	5.000889741633199E-4
27	8.334816236055332E-5	0.0	0.0	0.005667675040517625	5.000889741633199E-4
28	8.334816236055332E-5	0.0	0.0	0.012377202110542168	5.000889741633199E-4
29	8.334816236055332E-5	0.0	0.0	0.02467105605872378	5.000889741633199E-4
30	8.334816236055332E-5	0.0	0.0	0.05092572720229808	5.000889741633199E-4
31	8.334816236055332E-5	0.0	0.0	0.10647727741560686	5.000889741633199E-4
32	8.334816236055332E-5	0.0	0.0	0.16844663613067826	5.417630553435966E-4
33	8.334816236055332E-5	0.0	0.0	0.23395829174607316	5.417630553435966E-4
34	8.334816236055332E-5	0.0	0.0	0.3081381562469656	5.834371365238732E-4
35	8.334816236055332E-5	0.0	0.0	0.3915696667698795	5.834371365238732E-4
36	8.334816236055332E-5	0.0	0.0	0.5144665321705153	5.834371365238732E-4
37	1.2502224354082997E-4	0.0	0.0	0.6860387243897144	5.834371365238732E-4
38	1.2502224354082997E-4	0.0	0.0	0.9100785848148817	5.834371365238732E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGC	320	0.0	18.562473	36
CGCAATA	715	0.0	18.461512	36
TCTAGCG	720	0.0	18.333307	28
TTAACGG	325	0.0	18.276896	35
CTAGCGG	750	0.0	17.599976	29
TAGCGGC	770	0.0	16.857119	30
TACCGTC	575	0.0	16.834759	7
ATACGAA	785	0.0	16.815262	40
CAATACG	800	0.0	16.774977	38
GTATCAA	4250	0.0	16.772789	1
TACGAAT	830	0.0	16.168652	41
CGAATGC	805	0.0	16.1242	43
TAGGACG	345	0.0	15.942007	4
CGTCGTA	515	0.0	15.805803	10
GTATTAC	280	0.0	15.715245	1
ATACCGT	630	0.0	15.714263	6
AATACGA	845	0.0	15.62128	39
CGGCGCA	1185	0.0	15.594915	33
ACCGTCG	580	0.0	15.551701	8
TATTAGA	540	0.0	15.074052	2
>>END_MODULE