FastQCFastQC Report
Thu 2 Feb 2017
SRR4062565_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062565_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529240
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG101591.9195450079359082No Hit
TCCATGTACTCTGCGTTGATACCAC82941.5671528984959566No Hit
GAGTACATGGAAGCAGTGGTATCAA42530.803605169677273No Hit
CATGTACTCTGCGTTGATACCACTG35960.679464893054191No Hit
GCTTCCATGTACTCTGCGTTGATAC26980.5097876199833724No Hit
CCCATGTACTCTGCGTTGATACCAC25950.4903257501322651No Hit
GTACATGGGAAGCAGTGGTATCAAC24460.4621721714156149No Hit
CATGGAAGCAGTGGTATCAACGCAG22310.4215478799788376No Hit
GCGTTGATACCACTGCTTCCATGTA21910.4139898722696697No Hit
GTATCAACGCAGAGTACTTTTTTTT19440.3673191746655582No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18820.355604262716348No Hit
GTATCAACGCAGAGTACATGGAAGC16980.3208374272541758No Hit
TATCAACGCAGAGTACTTTTTTTTT15480.2924948983447963No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14920.2819136875519613No Hit
ACTCTGCGTTGATACCACTGCTTCC14820.28002418562466935No Hit
ACGCAGAGTACATGGAAGCAGTGGT14780.27926838485375255No Hit
CAGTGGTATCAACGCAGAGTACATG13660.25810596326808255No Hit
TATCAACGCAGAGTACATGGAAGCA11150.2106794648930542No Hit
GTACATGGGGTGGTATCAACGCAAA10520.1987756027511148No Hit
GTATCAACGCAGAGTACATGGGAAG10410.19669715063109364No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9440.17836898193636158No Hit
GAGTACATGGGAAGCAGTGGTATCA8360.15796236112160833No Hit
AAGCAGTGGTATCAACGCAGAGTAC8060.15229385533973244No Hit
GGTATCAACGCAGAGTACATGGAAG7740.14624744917239815No Hit
GGTATCAACGCAGAGTACTTTTTTT7590.1434131962814602No Hit
ATACCACTGCTTCCATGTACTCTGC7340.13868944146323028No Hit
GCGTTGATACCACTGCTTCCCATGT7320.1383115410777719No Hit
TTGTAGAACAGTGTATATCAATGAG6940.13113143375406242No Hit
GATATACACTGTTCTACAAATCCCG6400.12092812334668582No Hit
GTGGTATCAACGCAGAGTACATGGA6240.11790492026301866No Hit
GCTTCCCATGTACTCTGCGTTGATA6240.11790492026301866No Hit
GTGTATATCAATGAGTTACAATGAA6140.11601541833572669No Hit
TATCAACGCAGAGTACATGGGAAGC6020.11374801602297635No Hit
GGTATCAACGCAGAGTACATGGGAA6000.11337011563751795No Hit
GTACATGGGTGGTATCAACGCAAAA5980.11299221525205956No Hit
GTACTCTGCGTTGATACCACTGCTT5980.11299221525205956No Hit
GTCCTACAGTGTGCATTTCTCATTT5780.10921321139747563No Hit
CCACTGCTTCCATGTACTCTGCGTT5610.1060010581210793No Hit
GTCCTAAAGTGTGTATTTCTCATTT5510.10411155619378731No Hit
GAAGCAGTGGTATCAACGCAGAGTA5360.10127730330284936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCCG402.749473E-416.63079517
GGCGTTC402.7574238E-416.6245048
CGTTCAG402.7574238E-416.62450410
ACACTAC350.00216808516.2852298
GTAATAC350.002172198816.280613
GTATAGA508.733995E-515.192361
GAGGTGT400.005274053714.2495756
TTAAGCA400.005280677714.2468794
GTCCTAT1203.6379788E-1214.2428391
CCCCTAT400.005290627514.2428381
CCTAATA400.005290627514.2428382
TTGTAGA1500.013.9263311
GCACTGT707.2431158E-613.5710246
CCAACGT500.001493336513.30463719
CACCCGA500.001493336513.30463718
CGTTAAA500.001496534913.30086113
GCGTTCA500.001497602413.2996039
CCTACAC500.001500808613.295833
ACCATAC500.001502949413.2933151
TCCAACG1251.0186341E-1012.92450318
Produced by FastQC (version 0.11.5)