##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062565_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	529240
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.932387952535713	32.0	32.0	32.0	32.0	32.0
2	30.683657697830853	32.0	32.0	32.0	32.0	32.0
3	30.712727684982237	32.0	32.0	32.0	32.0	32.0
4	30.710420603129016	32.0	32.0	32.0	32.0	32.0
5	30.59107777189933	32.0	32.0	32.0	32.0	32.0
6	34.17785503741214	36.0	36.0	36.0	32.0	36.0
7	34.003988738568516	36.0	36.0	36.0	32.0	36.0
8	33.95916975285315	36.0	36.0	36.0	32.0	36.0
9	34.07686682790416	36.0	36.0	36.0	32.0	36.0
10	33.82196923890862	36.0	36.0	36.0	32.0	36.0
11	34.180039301640086	36.0	36.0	36.0	32.0	36.0
12	33.94641750434585	36.0	36.0	36.0	32.0	36.0
13	34.045298919204896	36.0	36.0	36.0	32.0	36.0
14	33.962565187816494	36.0	36.0	36.0	32.0	36.0
15	33.93523354243821	36.0	36.0	36.0	32.0	36.0
16	33.927530043080644	36.0	36.0	36.0	32.0	36.0
17	33.83440594059406	36.0	36.0	36.0	32.0	36.0
18	33.850814375330664	36.0	36.0	36.0	32.0	36.0
19	33.84035787166503	36.0	36.0	36.0	32.0	36.0
20	33.77503212153277	36.0	36.0	36.0	32.0	36.0
21	33.678074219635704	36.0	36.0	36.0	27.0	36.0
22	33.63609515531706	36.0	36.0	36.0	27.0	36.0
23	33.543246920111855	36.0	36.0	36.0	27.0	36.0
24	33.52786070591792	36.0	36.0	36.0	27.0	36.0
25	32.94918562466934	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	24.0
5	43.0
6	93.0
7	19.0
8	71.0
9	105.0
10	75.0
11	15.0
12	37.0
13	34.0
14	180.0
15	214.0
16	284.0
17	386.0
18	539.0
19	794.0
20	1144.0
21	1735.0
22	2379.0
23	3314.0
24	4336.0
25	5576.0
26	7256.0
27	9244.0
28	11901.0
29	15968.0
30	20064.0
31	27480.0
32	38439.0
33	54070.0
34	114015.0
35	209403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.26857687082029	16.58336372552169	11.765839684198392	24.38221971945963
2	13.971803946540495	21.291591635284178	38.66555308442754	26.07105133374778
3	20.197082498771778	26.291523374022148	27.896526964211482	25.6148671629946
4	11.996704222903382	16.46830271259085	35.32944542503052	36.20554763947524
5	12.688850875617847	38.72300611467617	34.313823704529774	14.274319305176212
6	33.23452962693409	34.23869790829781	18.49715025661868	14.029622208149414
7	28.013227628272634	31.438684195101846	21.965879676985704	18.582208499639812
8	28.312067038472232	33.69889801067003	20.996802018264997	16.99223293259275
9	27.237203953714456	15.2236163701633	18.1817150163225	39.35746465979974
10	15.33748018327866	28.69082887811818	33.03083273741284	22.940858201190327
11	36.6142320680294	21.681060168938412	21.200162695447368	20.50454506758482
12	22.83366981451593	24.583308893509855	30.743090467711998	21.839930824262215
13	31.5449243173808	20.459184735210155	25.01224959278381	22.983641354625238
14	23.87738074742273	19.887695506495913	25.37294513615217	30.861978609929185
15	25.721328003964867	27.176185062509106	25.5505132953619	21.551973638164128
16	24.957909432448897	25.810503535607676	27.142835523303987	22.088751508639444
17	22.943484611135077	25.97793266283742	28.54110382091856	22.537478905108937
18	24.717902145532978	24.090135089113407	30.238960154387556	20.953002610966056
19	23.74372548611518	28.867967010200047	25.297288501817317	22.091019001867455
20	23.252386591950476	24.37539965719345	30.222218858900675	22.149994891955398
21	24.471500875076867	26.69996688898349	23.9050186840736	24.92351355186604
22	22.77282998300549	27.611155058799486	25.73078276930996	23.885232188885062
23	22.23262960503036	26.619246846089943	25.402339958105554	25.745783590774142
24	22.228907683865962	28.857732114813345	25.662333730676806	23.251026470643886
25	24.832788733886698	25.584884669162932	24.816895385820484	24.765431211129886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	199.0
2	385.5
3	572.0
4	572.0
5	572.0
6	1574.5
7	2577.0
8	2577.0
9	2577.0
10	2569.0
11	2561.0
12	2561.0
13	2561.0
14	2203.5
15	1846.0
16	1846.0
17	1846.0
18	2975.5
19	4105.0
20	4105.0
21	4105.0
22	6544.0
23	8983.0
24	8983.0
25	8983.0
26	13502.0
27	18021.0
28	18021.0
29	18021.0
30	23517.0
31	29013.0
32	29013.0
33	29013.0
34	31832.5
35	34652.0
36	34652.0
37	34652.0
38	38011.0
39	41370.0
40	41370.0
41	41370.0
42	50031.5
43	58693.0
44	58693.0
45	58693.0
46	81804.0
47	104915.0
48	104915.0
49	104915.0
50	88043.5
51	71172.0
52	71172.0
53	71172.0
54	59014.0
55	46856.0
56	46856.0
57	46856.0
58	42216.0
59	37576.0
60	37576.0
61	37576.0
62	33041.0
63	28506.0
64	28506.0
65	28506.0
66	23358.5
67	18211.0
68	18211.0
69	18211.0
70	13723.0
71	9235.0
72	9235.0
73	9235.0
74	7103.5
75	4972.0
76	4972.0
77	4972.0
78	3976.5
79	2981.0
80	2981.0
81	2981.0
82	2055.5
83	1130.0
84	1130.0
85	1130.0
86	827.0
87	524.0
88	524.0
89	524.0
90	350.5
91	177.0
92	177.0
93	177.0
94	118.0
95	59.0
96	59.0
97	59.0
98	196.5
99	334.0
100	334.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008880659058272239
2	0.0032121532763963417
3	0.003779003854583932
4	0.014738115032877332
5	0.03495578565490137
6	0.04629279721865316
7	0.06556571687703121
8	0.0886176403899932
9	0.09863200060464061
10	0.12206182450306098
11	0.1218728743103318
12	0.1381225908850427
13	0.12300657546670697
14	0.1279192804776661
15	0.11280326505933036
16	0.11677121910664348
17	0.12773033028493688
18	0.1322651349104376
19	0.13472148741591716
20	0.12508502758672815
21	0.13509938780137556
22	0.15758446073614996
23	0.1453026982087522
24	0.1379336406923135
25	0.13547728818683397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	529240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.39591078897138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.01750506338254	39.82159135941321
2	13.215492926941774	13.320136051568799
3	3.648645086172211	5.516303767900606
4	1.4037264451744298	2.82968290812522
5	0.708711407056863	1.7858078422582029
6	0.40177178853518347	1.214859312752675
7	0.23835337518530028	0.8408424802462032
8	0.1743610867212022	0.7029668617175847
9	0.13682368606447562	0.6205818849051187
>10	0.84316296940325	8.41668647090833
>50	0.11550528965626376	4.035067723350832
>100	0.0793863716630899	7.620193442418042
>500	0.009029729478266151	2.9300268547534403
>1k	0.006772297108699612	6.846399383384276
>5k	3.762387282610896E-4	1.572616497335471
>10k+	3.762387282610896E-4	1.9262371589620266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10159	1.9195450079359082	No Hit
TCCATGTACTCTGCGTTGATACCAC	8294	1.5671528984959566	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4253	0.803605169677273	No Hit
CATGTACTCTGCGTTGATACCACTG	3596	0.679464893054191	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2698	0.5097876199833724	No Hit
CCCATGTACTCTGCGTTGATACCAC	2595	0.4903257501322651	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2446	0.4621721714156149	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2231	0.4215478799788376	No Hit
GCGTTGATACCACTGCTTCCATGTA	2191	0.4139898722696697	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1944	0.3673191746655582	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1882	0.355604262716348	No Hit
GTATCAACGCAGAGTACATGGAAGC	1698	0.3208374272541758	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1548	0.2924948983447963	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1492	0.2819136875519613	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1482	0.28002418562466935	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1478	0.27926838485375255	No Hit
CAGTGGTATCAACGCAGAGTACATG	1366	0.25810596326808255	No Hit
TATCAACGCAGAGTACATGGAAGCA	1115	0.2106794648930542	No Hit
GTACATGGGGTGGTATCAACGCAAA	1052	0.1987756027511148	No Hit
GTATCAACGCAGAGTACATGGGAAG	1041	0.19669715063109364	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	944	0.17836898193636158	No Hit
GAGTACATGGGAAGCAGTGGTATCA	836	0.15796236112160833	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	806	0.15229385533973244	No Hit
GGTATCAACGCAGAGTACATGGAAG	774	0.14624744917239815	No Hit
GGTATCAACGCAGAGTACTTTTTTT	759	0.1434131962814602	No Hit
ATACCACTGCTTCCATGTACTCTGC	734	0.13868944146323028	No Hit
GCGTTGATACCACTGCTTCCCATGT	732	0.1383115410777719	No Hit
TTGTAGAACAGTGTATATCAATGAG	694	0.13113143375406242	No Hit
GATATACACTGTTCTACAAATCCCG	640	0.12092812334668582	No Hit
GTGGTATCAACGCAGAGTACATGGA	624	0.11790492026301866	No Hit
GCTTCCCATGTACTCTGCGTTGATA	624	0.11790492026301866	No Hit
GTGTATATCAATGAGTTACAATGAA	614	0.11601541833572669	No Hit
TATCAACGCAGAGTACATGGGAAGC	602	0.11374801602297635	No Hit
GGTATCAACGCAGAGTACATGGGAA	600	0.11337011563751795	No Hit
GTACATGGGTGGTATCAACGCAAAA	598	0.11299221525205956	No Hit
GTACTCTGCGTTGATACCACTGCTT	598	0.11299221525205956	No Hit
GTCCTACAGTGTGCATTTCTCATTT	578	0.10921321139747563	No Hit
CCACTGCTTCCATGTACTCTGCGTT	561	0.1060010581210793	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	551	0.10411155619378731	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	536	0.10127730330284936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.8895019272919657E-4	0.0	0.0	0.0
6	0.0	1.8895019272919657E-4	0.0	0.0	0.0
7	0.0	1.8895019272919657E-4	0.0	0.0	0.0
8	0.0	1.8895019272919657E-4	0.0	0.0	0.0
9	0.0	1.8895019272919657E-4	0.0	0.0	0.0
10	0.0	1.8895019272919657E-4	0.0	0.0	0.0
11	0.0	1.8895019272919657E-4	0.0	3.7790038545839315E-4	0.0
12	0.0	1.8895019272919657E-4	0.0	5.668505781875897E-4	0.0
13	0.0	3.7790038545839315E-4	0.0	7.558007709167863E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACCCG	40	2.749473E-4	16.630795	17
GGCGTTC	40	2.7574238E-4	16.624504	8
CGTTCAG	40	2.7574238E-4	16.624504	10
ACACTAC	35	0.002168085	16.285229	8
GTAATAC	35	0.0021721988	16.28061	3
GTATAGA	50	8.733995E-5	15.19236	1
GAGGTGT	40	0.0052740537	14.249575	6
TTAAGCA	40	0.0052806777	14.246879	4
GTCCTAT	120	3.6379788E-12	14.242839	1
CCCCTAT	40	0.0052906275	14.242838	1
CCTAATA	40	0.0052906275	14.242838	2
TTGTAGA	150	0.0	13.926331	1
GCACTGT	70	7.2431158E-6	13.571024	6
CCAACGT	50	0.0014933365	13.304637	19
CACCCGA	50	0.0014933365	13.304637	18
CGTTAAA	50	0.0014965349	13.300861	13
GCGTTCA	50	0.0014976024	13.299603	9
CCTACAC	50	0.0015008086	13.29583	3
ACCATAC	50	0.0015029494	13.293315	1
TCCAACG	125	1.0186341E-10	12.924503	18
>>END_MODULE