FastQCFastQC Report
Thu 2 Feb 2017
SRR4062565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529240
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA45760.8646360819288036No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41670.7873554531025623No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA17720.33481974151613636No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC17650.33349709016703194No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10870.20538885949663668No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10570.1997203537147608No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10530.19896455294384402No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10060.19008389388557176No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9660.1825258861764039No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9400.17761318116544478No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.1713778248053813No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8100.15304965611064925No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA7740.14624744917239815No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC6090.11507066737208073No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG5980.11299221525205956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.10864636081928804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGA300.00574219329.3332716
TCGTATA655.9207196E-727.07686444
TAGGACG3050.025.9671574
TGGTATA2900.025.79304744
GTAGGAC4050.025.530813
TTAGGAC1900.025.473633
TGTAGGA4100.024.6828752
GACGTGA1700.024.5881827
ATTCGTG450.001397193524.44439344
CTTACAC450.001397193524.4443933
CTGTAGG3850.024.0022161
GGACGTG3100.023.4193046
GATATAC3250.022.3405231
ATACACT3450.022.3187924
CTAAGAC703.2110034E-521.9999523
CGAATGT2250.021.51106633
TAGGACC3500.021.3713824
ACGTATG1158.743882E-921.04343422
TGGCGAG3900.020.8717518
AGGACGT3500.020.7428115
Produced by FastQC (version 0.11.5)