FastQCFastQC Report
Thu 2 Feb 2017
SRR4062564_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062564_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054744
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG103600.9822288631174958No Hit
TCCATGTACTCTGCGTTGATACCAC82010.7775346434774695No Hit
GAGTACATGGAAGCAGTGGTATCAA41590.3943136912843306No Hit
CATGTACTCTGCGTTGATACCACTG35800.33941885424330454No Hit
GCTTCCATGTACTCTGCGTTGATAC27080.2567447646063879No Hit
CCCATGTACTCTGCGTTGATACCAC24540.23266309170756125No Hit
GTATCAACGCAGAGTACTTTTTTTT24440.23171499434933973No Hit
CATGGAAGCAGTGGTATCAACGCAG22600.21427000295806375No Hit
GCGTTGATACCACTGCTTCCATGTA22250.21095166220428843No Hit
GTACATGGGAAGCAGTGGTATCAAC21760.20630598514900297No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18940.17956963964715608No Hit
TATCAACGCAGAGTACTTTTTTTTT18280.17331219708289405No Hit
GTATCAACGCAGAGTACATGGAAGC16700.158332258822994No Hit
ACTCTGCGTTGATACCACTGCTTCC14560.13804297535705345No Hit
ACGCAGAGTACATGGAAGCAGTGGT14040.13311286909430153No Hit
CAGTGGTATCAACGCAGAGTACATG13700.1298893380763484No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13480.12780352388826105No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11810.11197029800596163No Hit
TATCAACGCAGAGTACATGGAAGCA11660.11054815196862935No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11090.10514399702676669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTA307.729946E-418.9965364
AGAACCG953.0559022E-1014.9986885
AACCGCG851.7142156E-814.529527
CGCGCTA400.00526904214.253489516
CCCAATT602.5642323E-514.2514613
CTCGTAG903.6294296E-813.72362810
CGCATCG1750.013.57281913
GCTCGTA852.6928683E-713.4131389
GCGTTAT852.7178794E-713.4029581
CTATTAG500.001506426813.2912661
GACGGAG655.448852E-513.1539447
TTAGTAC655.4682983E-513.1489523
CGCAAGA1550.012.8637372
CGAACGA604.08164E-412.66976816
AAGACGG1800.012.6655595
ATAATAC751.4831778E-512.6619533
CGCCAGT1900.012.503654518
GAACCGC1301.9826984E-1012.423176
ATTTGCG1001.4361103E-712.353024516
CGCGGTC1001.438766E-712.35126610
Produced by FastQC (version 0.11.5)