##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062564_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1054744
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02646803394947	32.0	32.0	32.0	32.0	32.0
2	30.598518692687513	32.0	32.0	32.0	32.0	32.0
3	30.604152287190068	32.0	32.0	32.0	32.0	32.0
4	30.674649962455344	32.0	32.0	32.0	32.0	32.0
5	30.428456573348605	32.0	32.0	32.0	32.0	32.0
6	34.06772543858984	36.0	36.0	36.0	32.0	36.0
7	33.92795597794346	36.0	36.0	36.0	32.0	36.0
8	33.9017647884226	36.0	36.0	36.0	32.0	36.0
9	34.10810111268706	36.0	36.0	36.0	32.0	36.0
10	33.69859321313987	36.0	36.0	36.0	27.0	36.0
11	34.13994770294972	36.0	36.0	36.0	32.0	36.0
12	33.83879690237631	36.0	36.0	36.0	32.0	36.0
13	33.979914557466074	36.0	36.0	36.0	32.0	36.0
14	33.866938328163045	36.0	36.0	36.0	32.0	36.0
15	33.79832547044591	36.0	36.0	36.0	32.0	36.0
16	33.79321143329566	36.0	36.0	36.0	32.0	36.0
17	33.691259680074026	36.0	36.0	36.0	27.0	36.0
18	33.713159780951585	36.0	36.0	36.0	27.0	36.0
19	33.704514081141966	36.0	36.0	36.0	27.0	36.0
20	33.69286196460942	36.0	36.0	36.0	27.0	36.0
21	33.63468671070895	36.0	36.0	36.0	27.0	36.0
22	33.58259255326411	36.0	36.0	36.0	27.0	36.0
23	33.49406775482961	36.0	36.0	36.0	27.0	36.0
24	33.483673763491424	36.0	36.0	36.0	27.0	36.0
25	32.93272395955796	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	15.0
4	37.0
5	89.0
6	169.0
7	42.0
8	175.0
9	185.0
10	135.0
11	45.0
12	67.0
13	55.0
14	240.0
15	290.0
16	414.0
17	558.0
18	810.0
19	1281.0
20	1934.0
21	2853.0
22	4341.0
23	6416.0
24	8612.0
25	11903.0
26	15974.0
27	20070.0
28	26035.0
29	34234.0
30	43588.0
31	58312.0
32	82097.0
33	113862.0
34	233212.0
35	386694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.62915648553497	16.850851517142704	11.296386373829348	25.22360562349298
2	15.780905561270686	20.445527422359806	37.624549044519554	26.149017971849947
3	19.288697573209063	24.910091638735736	28.17078084925831	27.63042993879689
4	12.111698173322004	15.71925446049278	35.89720005461828	36.271847311566944
5	13.64914130933729	37.47453518412437	34.09990174469554	14.776421761842798
6	33.95468307748344	34.99977235303491	17.514208964737485	13.531335604744163
7	29.32662523279278	30.846478674141338	21.38714613836738	18.4397499546985
8	28.419491392734425	32.79870641922379	20.14748263221673	18.63431955582505
9	27.14664642103016	14.704405798476833	18.293482644902607	39.85546513559041
10	15.198130418852243	27.56512114878244	32.417811899025914	24.818936533339407
11	37.14309038166548	21.44267424023814	21.55287957717601	19.86135580092037
12	23.397117013116222	24.17131215396183	29.777700142022802	22.65387069089915
13	30.204146724406666	19.930457597740016	25.032227198426515	24.8331684794268
14	23.72541388213852	19.70230862697448	25.24168438639125	31.330593104495748
15	25.5363254799451	26.805073854500655	23.953688358875354	23.704912306678892
16	25.977123646396986	25.72037924138435	24.777021518123497	23.525475594095163
17	23.74965352530879	25.873306070995987	26.47398876852453	23.903051635170694
18	24.932122487108018	24.511756575100062	27.606591428636523	22.949529509155393
19	24.54346785952974	27.152033082204262	25.16879063126557	23.135708427000427
20	24.401875723805368	24.325176086419987	27.300514495092365	23.972433694682284
21	25.778043158802067	25.454428087016286	24.02678154416316	24.740747210018494
22	24.236922813980648	25.871416770321982	25.115033660282766	24.776626755414604
23	23.343789578819646	25.213096637354997	25.240724670979553	26.202389112845804
24	23.392303639323874	26.888655771321158	25.130369153799055	24.588671435555913
25	24.88833574936324	25.0595938221783	24.613506414108475	25.438564014349986
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	203.0
1	203.0
2	480.5
3	758.0
4	758.0
5	758.0
6	1917.0
7	3076.0
8	3076.0
9	3076.0
10	3148.0
11	3220.0
12	3220.0
13	3220.0
14	3317.5
15	3415.0
16	3415.0
17	3415.0
18	5480.0
19	7545.0
20	7545.0
21	7545.0
22	11877.5
23	16210.0
24	16210.0
25	16210.0
26	24058.0
27	31906.0
28	31906.0
29	31906.0
30	39907.0
31	47908.0
32	47908.0
33	47908.0
34	57828.5
35	67749.0
36	67749.0
37	67749.0
38	76443.0
39	85137.0
40	85137.0
41	85137.0
42	99638.0
43	114139.0
44	114139.0
45	114139.0
46	143167.5
47	172196.0
48	172196.0
49	172196.0
50	157041.0
51	141886.0
52	141886.0
53	141886.0
54	124784.5
55	107683.0
56	107683.0
57	107683.0
58	98302.5
59	88922.0
60	88922.0
61	88922.0
62	78267.5
63	67613.0
64	67613.0
65	67613.0
66	56458.0
67	45303.0
68	45303.0
69	45303.0
70	34071.5
71	22840.0
72	22840.0
73	22840.0
74	17995.5
75	13151.0
76	13151.0
77	13151.0
78	10615.0
79	8079.0
80	8079.0
81	8079.0
82	5633.0
83	3187.0
84	3187.0
85	3187.0
86	2290.5
87	1394.0
88	1394.0
89	1394.0
90	991.5
91	589.0
92	589.0
93	589.0
94	364.0
95	139.0
96	139.0
97	139.0
98	317.5
99	496.0
100	496.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009196544374748754
2	0.003697579697063932
3	0.0055937744135069745
4	0.01441107984496712
5	0.032993788066108934
6	0.04550867319463301
7	0.06589276639639571
8	0.0876990056354907
9	0.096611120802773
10	0.11813293083440153
11	0.11908102819262305
12	0.13197515226443574
13	0.12192532026728761
14	0.12363189551208634
15	0.1124443466850724
16	0.1106429617044515
17	0.1209772229090661
18	0.1304581964912813
19	0.12969971860470408
20	0.12173570079564332
21	0.1262865681151066
22	0.14923052418406743
23	0.138991072715275
24	0.13169072305696927
25	0.1292256699255933
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1054744.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.18264972254044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4019578116376	42.22806509580257
2	12.84932338014118	13.667221289953957
3	3.67907487273003	5.869888507782038
4	1.418651314248555	3.017905436964102
5	0.7137765181613018	1.8980263272773519
6	0.42555996492450776	1.3579443930310016
7	0.2611616884308957	0.9722489417737299
8	0.1779078546321359	0.7569288892631623
9	0.13250209486095801	0.6342131248643841
>10	0.7500789339270939	7.553273306622768
>50	0.08857991461058662	3.334597575800202
>100	0.0871403489505004	9.472126976142672
>500	0.009999711921315902	3.6255277290822097
>1k	0.003928458254802675	3.849363254593558
>5k	1.7856628430921252E-4	0.7788184746366451
>10k+	1.7856628430921252E-4	0.9838506764096626
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10360	0.9822288631174958	No Hit
TCCATGTACTCTGCGTTGATACCAC	8201	0.7775346434774695	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4159	0.3943136912843306	No Hit
CATGTACTCTGCGTTGATACCACTG	3580	0.33941885424330454	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2708	0.2567447646063879	No Hit
CCCATGTACTCTGCGTTGATACCAC	2454	0.23266309170756125	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2444	0.23171499434933973	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2260	0.21427000295806375	No Hit
GCGTTGATACCACTGCTTCCATGTA	2225	0.21095166220428843	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2176	0.20630598514900297	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1894	0.17956963964715608	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1828	0.17331219708289405	No Hit
GTATCAACGCAGAGTACATGGAAGC	1670	0.158332258822994	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1456	0.13804297535705345	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1404	0.13311286909430153	No Hit
CAGTGGTATCAACGCAGAGTACATG	1370	0.1298893380763484	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1348	0.12780352388826105	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1181	0.11197029800596163	No Hit
TATCAACGCAGAGTACATGGAAGCA	1166	0.11054815196862935	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1109	0.10514399702676669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.48097358221521E-5	0.0
10	0.0	0.0	0.0	9.48097358221521E-5	0.0
11	0.0	0.0	0.0	9.48097358221521E-5	0.0
12	0.0	0.0	0.0	9.48097358221521E-5	1.896194716443042E-4
13	0.0	0.0	0.0	9.48097358221521E-5	1.896194716443042E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGTA	30	7.729946E-4	18.996536	4
AGAACCG	95	3.0559022E-10	14.998688	5
AACCGCG	85	1.7142156E-8	14.52952	7
CGCGCTA	40	0.005269042	14.2534895	16
CCCAATT	60	2.5642323E-5	14.25146	13
CTCGTAG	90	3.6294296E-8	13.723628	10
CGCATCG	175	0.0	13.572819	13
GCTCGTA	85	2.6928683E-7	13.413138	9
GCGTTAT	85	2.7178794E-7	13.402958	1
CTATTAG	50	0.0015064268	13.291266	1
GACGGAG	65	5.448852E-5	13.153944	7
TTAGTAC	65	5.4682983E-5	13.148952	3
CGCAAGA	155	0.0	12.863737	2
CGAACGA	60	4.08164E-4	12.669768	16
AAGACGG	180	0.0	12.665559	5
ATAATAC	75	1.4831778E-5	12.661953	3
CGCCAGT	190	0.0	12.5036545	18
GAACCGC	130	1.9826984E-10	12.42317	6
ATTTGCG	100	1.4361103E-7	12.3530245	16
CGCGGTC	100	1.438766E-7	12.351266	10
>>END_MODULE