FastQCFastQC Report
Thu 2 Feb 2017
SRR4062564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054744
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41040.3890991558141122No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35600.3375226595268615No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15610.14799799761837942No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC14180.13444020539581167No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12190.11557306796720342No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11230.10647133332827681No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10640.10087755891476985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC3050.024.52456530
CTAGCGG3250.023.01535829
AGTATCG500.002580237621.9999775
TCTAGCG3500.021.99997528
CGCAATA3550.021.69011936
CGGTCCA3850.021.14283410
CAATACG3650.021.09586738
ATACGAA3850.020.57140540
CGAATGC3750.020.53331243
TCTAGTA654.9316254E-420.307672
CCGGTCC4350.020.2298629
AATACGA4050.019.55553439
AGCGGCG3850.019.4285531
TGGTATA3400.019.41174544
TCGTATA808.987262E-519.24997944
GTATAAG1950.019.1803741
GCCGGTC4150.019.0843168
CGCCGGT3850.018.8571227
ACTAGAC600.00741153818.3333133
CTAGACT1456.3046173E-918.2068774
Produced by FastQC (version 0.11.5)