##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1054744
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195967931554957	32.0	32.0	32.0	32.0	32.0
2	31.3552113119392	32.0	32.0	32.0	32.0	32.0
3	31.47704371866538	32.0	32.0	32.0	32.0	32.0
4	31.595532186009116	32.0	32.0	32.0	32.0	32.0
5	31.521344515825643	32.0	32.0	32.0	32.0	32.0
6	35.10554693840401	36.0	36.0	36.0	36.0	36.0
7	35.11805329065631	36.0	36.0	36.0	36.0	36.0
8	35.07199472099391	36.0	36.0	36.0	36.0	36.0
9	35.194369439408995	36.0	36.0	36.0	36.0	36.0
10	35.06838815864324	36.0	36.0	36.0	36.0	36.0
11	35.21049467927762	36.0	36.0	36.0	36.0	36.0
12	35.10440542918471	36.0	36.0	36.0	36.0	36.0
13	35.15312151574221	36.0	36.0	36.0	36.0	36.0
14	35.10186073587525	36.0	36.0	36.0	36.0	36.0
15	35.06471333328277	36.0	36.0	36.0	36.0	36.0
16	35.0733770469422	36.0	36.0	36.0	36.0	36.0
17	35.044025848926374	36.0	36.0	36.0	36.0	36.0
18	35.04970021161533	36.0	36.0	36.0	36.0	36.0
19	35.02790914193397	36.0	36.0	36.0	36.0	36.0
20	35.02623954248614	36.0	36.0	36.0	36.0	36.0
21	34.987475633897894	36.0	36.0	36.0	36.0	36.0
22	34.95734415175625	36.0	36.0	36.0	36.0	36.0
23	34.89190457589709	36.0	36.0	36.0	32.0	36.0
24	34.8651578013243	36.0	36.0	36.0	32.0	36.0
25	34.83358805549024	36.0	36.0	36.0	32.0	36.0
26	34.77097286166122	36.0	36.0	36.0	32.0	36.0
27	34.74533441290019	36.0	36.0	36.0	32.0	36.0
28	34.70138725605455	36.0	36.0	36.0	32.0	36.0
29	34.6642891545247	36.0	36.0	36.0	32.0	36.0
30	34.64044260977071	36.0	36.0	36.0	32.0	36.0
31	34.619335118284624	36.0	36.0	36.0	32.0	36.0
32	34.57833844041777	36.0	36.0	36.0	32.0	36.0
33	34.53434387870421	36.0	36.0	36.0	32.0	36.0
34	34.5161555789841	36.0	36.0	36.0	32.0	36.0
35	34.43931134000288	36.0	36.0	36.0	32.0	36.0
36	34.3968014987523	36.0	36.0	36.0	32.0	36.0
37	34.333454373762734	36.0	36.0	36.0	32.0	36.0
38	34.27011293735731	36.0	36.0	36.0	32.0	36.0
39	34.155203537540864	36.0	36.0	36.0	32.0	36.0
40	34.07507508931077	36.0	36.0	36.0	32.0	36.0
41	34.07444081217812	36.0	36.0	36.0	32.0	36.0
42	33.9236639412028	36.0	36.0	36.0	32.0	36.0
43	33.97101855995388	36.0	36.0	36.0	32.0	36.0
44	33.95043821059897	36.0	36.0	36.0	32.0	36.0
45	33.77300463429989	36.0	36.0	36.0	32.0	36.0
46	33.89844170718203	36.0	36.0	36.0	32.0	36.0
47	33.78376079882891	36.0	36.0	36.0	32.0	36.0
48	33.79036050453949	36.0	36.0	36.0	32.0	36.0
49	33.834829114932155	36.0	36.0	36.0	32.0	36.0
50	33.2370783811048	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	22.0
22	133.0
23	380.0
24	1034.0
25	2410.0
26	5229.0
27	9965.0
28	15843.0
29	22499.0
30	30589.0
31	42253.0
32	61400.0
33	85403.0
34	179545.0
35	598033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.096639177388816	17.383293839424308	11.815079371852459	25.704987611334417
2	15.27697976103112	20.85943085129582	37.11138164668938	26.752207740983675
3	18.717083026384778	25.062361517610547	28.626093519047874	27.594461936956794
4	12.00606404220179	16.091612925246174	36.0735611173159	35.82876191523614
5	13.479018111521004	37.5577747375427	34.02326443503299	14.939942715903307
6	34.139757930405466	35.34583944069523	17.08177765473511	13.4326249741642
7	29.187556411792816	30.92693582518602	21.309341413651083	18.576166349370084
8	28.391249440622556	32.926567963411024	20.055103418459836	18.62707917750658
9	27.27495961105254	14.343480503325926	18.185076189103707	40.196483696517824
10	15.146898204682843	27.59930371730012	32.48039334663198	24.773404731385057
11	37.4691868358578	21.152810539808712	21.558501399391698	19.81950122494179
12	23.3753403669516	24.14453175367672	29.763146317969095	22.716981561402577
13	30.445397176945306	19.63101947012735	25.225078312841788	24.698505040085557
14	23.721680331909923	19.488141198243365	25.32917940277451	31.460999067072198
15	25.627450831671002	26.73179463452743	23.87536691367763	23.765387620123935
16	25.891875184878987	25.714770598363206	24.760131368369954	23.633222848387856
17	23.70451976972611	25.785498661286532	26.54587274258019	23.964108826407166
18	25.15302291361695	24.351596216712302	27.632961173516986	22.862419696153758
19	24.760700226784888	26.8519185698141	25.301494959914443	23.085886243486573
20	24.54225859545065	24.04962720811875	27.525352123358843	23.88276207307176
21	25.915198379891237	25.25058213177795	24.252235613570686	24.581983874760134
22	24.307130450611712	25.552645950107323	25.301684579386084	24.838539019894874
23	23.401413044302693	24.80004626715108	25.420196749163775	26.378343939382447
24	23.545713462224008	26.623711535690177	25.218157202126772	24.61241779995904
25	25.02076333214505	24.59440395015283	25.002085814188085	25.382746903514033
26	23.38795006181595	25.257124003549674	26.18322550306046	25.171700431573917
27	25.390995350530552	25.461913032925526	25.519746971777035	23.627344644766882
28	24.17809440015776	24.225783697276306	26.09656940451901	25.499552498046917
29	23.458393695531807	25.66935673490439	26.714160023664512	24.15808954589929
30	24.88850375067315	24.953448419711325	26.52160145020972	23.63644637940581
31	23.808526049923014	25.219768967635748	25.52164316649348	25.450061815947755
32	23.73334192941605	25.580804441646503	25.714960217834847	24.970893411102598
33	23.647349499025356	24.885469839126838	25.947244070599123	25.519936591248683
34	23.63331765812368	25.830248856594583	26.826319941142117	23.71011354413962
35	25.452052820400024	24.985209681211746	25.98545239413545	23.577285104252784
36	24.05417807543821	26.03200397442413	25.689646018370333	24.22417193176733
37	25.417826505768225	25.593319326775028	25.656367801096756	23.33248636635999
38	24.270249463376896	25.305761398026437	25.254090092003366	25.169899046593297
39	24.276222476733693	25.24593645472266	25.81014919260029	24.66769187594336
40	25.18886099375773	25.435081877687853	25.15776340040806	24.218293728146357
41	23.287830980787756	25.60611864111102	26.561990397669955	24.54405998043127
42	25.936246141243753	25.82882671055725	25.563169830783583	22.671757317415413
43	24.725336195323226	24.121398178136115	25.468644524168898	25.684621102371764
44	24.159606501672442	25.24233368476142	26.49088309580334	24.107176717762794
45	24.79606425824655	25.30424444225329	25.589432127606322	24.310259171893843
46	23.74116134561817	25.019862677318244	26.47728069992472	24.761695277138866
47	24.57534719325258	24.582173494231778	26.881404397654784	23.96107491486086
48	24.598006720114075	27.048364342437598	24.5048087498009	23.84882018764743
49	23.39335421675781	25.4237047093892	27.116722161965367	24.066218911887624
50	23.581005041038434	27.19534521679689	24.35920409047512	24.86444565168956
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	17.0
2	20.0
3	88.0
4	156.0
5	186.5
6	217.0
7	189.0
8	161.0
9	249.5
10	338.0
11	484.5
12	631.0
13	1195.0
14	1759.0
15	2336.5
16	2914.0
17	3414.5
18	3915.0
19	4243.5
20	4572.0
21	5216.0
22	5860.0
23	6418.0
24	6976.0
25	7568.0
26	8160.0
27	10121.0
28	12082.0
29	13921.5
30	15761.0
31	17319.0
32	18877.0
33	21488.5
34	24100.0
35	27341.0
36	30582.0
37	35702.0
38	40822.0
39	43781.5
40	46741.0
41	50959.5
42	55178.0
43	58199.0
44	61220.0
45	70573.5
46	79927.0
47	86852.5
48	93778.0
49	91094.5
50	88411.0
51	79869.5
52	71328.0
53	68024.0
54	64720.0
55	64889.5
56	65059.0
57	63093.5
58	61128.0
59	55946.5
60	50765.0
61	44472.0
62	38179.0
63	33358.0
64	28537.0
65	24650.5
66	20764.0
67	18197.5
68	15631.0
69	14537.5
70	13444.0
71	10500.0
72	7556.0
73	6666.5
74	5777.0
75	4277.5
76	2778.0
77	2462.0
78	2146.0
79	1847.5
80	1549.0
81	1221.5
82	894.0
83	773.5
84	653.0
85	526.5
86	400.0
87	288.5
88	177.0
89	111.0
90	45.0
91	30.5
92	16.0
93	13.5
94	11.0
95	9.5
96	8.0
97	6.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014316270109144968
2	0.004361247847818997
3	6.636681507550648E-4
4	1.896194716443042E-4
5	9.48097358221521E-5
6	5.688584149329127E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.896194716443042E-4
47	0.0
48	0.0
49	0.0
50	9.48097358221521E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1054744.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.073084694266164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.31475047837931	45.26737471557129
2	12.979428331702678	14.815520249168493
3	3.6334822230705535	6.221221159572485
4	1.4580850944478014	3.3286965634746575
5	0.7345167902371983	2.0960569489284073
6	0.40920394043112596	1.4012718689671857
7	0.25950851154152604	1.0367665880664723
8	0.17937313132211194	0.8189902332658097
9	0.13405653974091924	0.6885918221808368
>10	0.7314114214624535	7.792932040054278
>50	0.08076086684433834	3.252966383113793
>100	0.07689712111616344	8.91776085164019
>500	0.007188208573740463	2.923292831018799
>1k	0.0013373411299982256	1.4385577449772016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4104	0.3890991558141122	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3560	0.3375226595268615	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1561	0.14799799761837942	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1418	0.13444020539581167	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1219	0.11557306796720342	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1123	0.10647133332827681	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1064	0.10087755891476985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.48097358221521E-5
7	0.0	0.0	0.0	0.0	9.48097358221521E-5
8	0.0	0.0	0.0	0.0	9.48097358221521E-5
9	0.0	0.0	0.0	9.48097358221521E-5	9.48097358221521E-5
10	0.0	0.0	0.0	9.48097358221521E-5	9.48097358221521E-5
11	0.0	0.0	0.0	9.48097358221521E-5	9.48097358221521E-5
12	0.0	0.0	0.0	9.48097358221521E-5	2.8442920746645634E-4
13	0.0	0.0	0.0	9.48097358221521E-5	2.8442920746645634E-4
14	0.0	0.0	0.0	1.896194716443042E-4	2.8442920746645634E-4
15	0.0	0.0	0.0	1.896194716443042E-4	2.8442920746645634E-4
16	0.0	0.0	0.0	1.896194716443042E-4	2.8442920746645634E-4
17	0.0	0.0	0.0	1.896194716443042E-4	2.8442920746645634E-4
18	0.0	0.0	0.0	2.8442920746645634E-4	2.8442920746645634E-4
19	0.0	0.0	0.0	4.7404867911076054E-4	2.8442920746645634E-4
20	0.0	0.0	0.0	8.532876223993689E-4	4.7404867911076054E-4
21	0.0	9.48097358221521E-5	0.0	0.0015169557731544337	4.7404867911076054E-4
22	0.0	9.48097358221521E-5	0.0	0.0025598628671981067	4.7404867911076054E-4
23	0.0	9.48097358221521E-5	0.0	0.004361247847818997	4.7404867911076054E-4
24	0.0	9.48097358221521E-5	0.0	0.00815363728070508	4.7404867911076054E-4
25	0.0	9.48097358221521E-5	0.0	0.01033426120461458	4.7404867911076054E-4
26	0.0	9.48097358221521E-5	0.0	0.013178553279279142	4.7404867911076054E-4
27	0.0	9.48097358221521E-5	0.0	0.016591703768876618	4.7404867911076054E-4
28	0.0	1.896194716443042E-4	0.0	0.02341800474807157	4.7404867911076054E-4
29	0.0	1.896194716443042E-4	0.0	0.03109759334966589	4.7404867911076054E-4
30	0.0	1.896194716443042E-4	0.0	0.044750195308055796	4.7404867911076054E-4
31	0.0	1.896194716443042E-4	0.0	0.07556335945025523	4.7404867911076054E-4
32	0.0	1.896194716443042E-4	0.0	0.112823585628361	4.7404867911076054E-4
33	0.0	2.8442920746645634E-4	0.0	0.15046305074975538	4.7404867911076054E-4
34	0.0	2.8442920746645634E-4	0.0	0.19682501156678778	5.688584149329127E-4
35	0.0	2.8442920746645634E-4	0.0	0.24840150785403853	5.688584149329127E-4
36	0.0	2.8442920746645634E-4	0.0	0.3233960088893608	5.688584149329127E-4
37	0.0	2.8442920746645634E-4	0.0	0.43365973165052374	5.688584149329127E-4
38	0.0	3.792389432886084E-4	0.0	0.5633594502552278	5.688584149329127E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	305	0.0	24.524565	30
CTAGCGG	325	0.0	23.015358	29
AGTATCG	50	0.0025802376	21.999977	5
TCTAGCG	350	0.0	21.999975	28
CGCAATA	355	0.0	21.690119	36
CGGTCCA	385	0.0	21.142834	10
CAATACG	365	0.0	21.095867	38
ATACGAA	385	0.0	20.571405	40
CGAATGC	375	0.0	20.533312	43
TCTAGTA	65	4.9316254E-4	20.30767	2
CCGGTCC	435	0.0	20.229862	9
AATACGA	405	0.0	19.555534	39
AGCGGCG	385	0.0	19.42855	31
TGGTATA	340	0.0	19.411745	44
TCGTATA	80	8.987262E-5	19.249979	44
GTATAAG	195	0.0	19.180374	1
GCCGGTC	415	0.0	19.084316	8
CGCCGGT	385	0.0	18.857122	7
ACTAGAC	60	0.007411538	18.333313	3
CTAGACT	145	6.3046173E-9	18.206877	4
>>END_MODULE