FastQCFastQC Report
Thu 2 Feb 2017
SRR4062563_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062563_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965958
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32400.16480514843145172No Hit
TATCAACGCAGAGTACTTTTTTTTT20900.10630949389559695No Hit
GCGCAAGACGGACCAGAGCGAAAGC20750.10554650709730319No Hit
GATTAAGAGGGACGGCCGGGGGCAT20390.10371533878139817No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19930.10137551259996398No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19900.10122291524030523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCC500.001500610713.2990374
AATCGTA905.407128E-712.666718513
ACGGTAT4300.012.5930749
CGTCTTA1800.012.13924715
GCGTTAT1603.6379788E-1211.8699091
CGATAAC1459.822543E-1111.7931510
CGCAAGA3650.011.7087982
AGTTAAA2600.011.69563117
GTCTAAT658.053993E-411.6872951
GTTCAAA5000.011.5850311
CGGTCCA3750.011.40004510
AATCCCC4050.011.25930511
CGAACGT1354.7348294E-911.2584444
TCGAACG1354.7493813E-911.2564373
CGACCAT3400.011.17651610
TTAGAAC1451.2223609E-911.135143
GTATAGA1451.2259989E-911.1331561
AAGACGG4200.011.0828145
ATCCCCG4050.011.02501712
CGCAGTT951.35631E-511.00312717
Produced by FastQC (version 0.11.5)