##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062563_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1965958
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.175542915972773	32.0	32.0	32.0	32.0	32.0
2	30.778848276514555	32.0	32.0	32.0	32.0	32.0
3	30.7460947792374	32.0	32.0	32.0	32.0	32.0
4	30.834607860391728	32.0	32.0	32.0	32.0	32.0
5	30.597649085077098	32.0	32.0	32.0	32.0	32.0
6	34.26506670030591	36.0	36.0	36.0	32.0	36.0
7	34.14146283898232	36.0	36.0	36.0	32.0	36.0
8	34.15087046620528	36.0	36.0	36.0	32.0	36.0
9	34.32609038443344	36.0	36.0	36.0	32.0	36.0
10	33.94304456148097	36.0	36.0	36.0	32.0	36.0
11	34.30965208819313	36.0	36.0	36.0	32.0	36.0
12	34.04773347141699	36.0	36.0	36.0	32.0	36.0
13	34.18695719847525	36.0	36.0	36.0	32.0	36.0
14	34.091251186444474	36.0	36.0	36.0	32.0	36.0
15	34.00245529151691	36.0	36.0	36.0	32.0	36.0
16	34.01573634838588	36.0	36.0	36.0	32.0	36.0
17	33.93724077523528	36.0	36.0	36.0	32.0	36.0
18	33.94753702774932	36.0	36.0	36.0	32.0	36.0
19	33.93954601268186	36.0	36.0	36.0	32.0	36.0
20	33.944023727872114	36.0	36.0	36.0	32.0	36.0
21	33.92098050924791	36.0	36.0	36.0	32.0	36.0
22	33.877595045265466	36.0	36.0	36.0	32.0	36.0
23	33.840239720278866	36.0	36.0	36.0	32.0	36.0
24	33.80049573795574	36.0	36.0	36.0	32.0	36.0
25	33.35149275823797	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	15.0
4	77.0
5	197.0
6	312.0
7	84.0
8	271.0
9	317.0
10	218.0
11	79.0
12	131.0
13	103.0
14	285.0
15	369.0
16	565.0
17	735.0
18	1036.0
19	1490.0
20	2406.0
21	3675.0
22	5808.0
23	8764.0
24	12620.0
25	18211.0
26	25086.0
27	32696.0
28	43924.0
29	57982.0
30	74435.0
31	100801.0
32	143620.0
33	204956.0
34	445239.0
35	779451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31368433475805	17.341891150828737	10.91635525286326	26.428069261549954
2	17.414716700688597	19.892272656289343	36.052340696753475	26.640669946268584
3	19.035769132749735	23.541234179291777	28.320015138435807	29.102981549522678
4	13.069229059101179	15.05680271745462	35.52607949582406	36.347888727620145
5	15.15670373877571	35.85341243361596	33.272239067527146	15.717644760081189
6	34.400954666083145	35.05349641364114	16.634394949836775	13.911153970438939
7	30.30457886271556	30.299896166296804	20.89582018445752	18.49970478653012
8	28.369487913487625	32.036652298506795	19.74431565084099	19.849544137164592
9	27.594970865333945	14.103469430874854	18.616707948201643	39.684851755589555
10	16.27101778586684	26.043823528513098	31.24397182045675	26.441186865163306
11	37.622384388574574	21.04527700400776	22.044925624716733	19.287412982700936
12	24.732559584563322	23.635702455093845	28.29271050428129	23.339027456061544
13	29.42644013798681	19.118424416372235	25.246910625330376	26.208224820310583
14	23.76642014591611	19.505457849229522	24.571423624231894	32.15669838062247
15	25.607321775386932	26.55060174581141	22.066559187202678	25.77551729159898
16	26.241308460232148	25.457994179755428	23.11136118869457	25.189336171317855
17	24.353810576041173	25.39239886190198	24.911961385741794	25.341829176315056
18	25.34066398600419	24.440104000876012	25.653736162714004	24.565495850405792
19	25.828238544109226	24.843540228292735	24.990374057014105	24.337847170583935
20	25.750534850453217	24.09650886901982	24.625833849807986	25.52712243071898
21	27.005672103812184	23.89261047666146	24.08899758539605	25.01271983413031
22	26.0444557195196	24.115602802195788	24.43352935662791	25.4064121216567
23	24.800280351172443	23.88475855366504	25.12525136864753	26.189709726514987
24	24.928185653836515	24.985178741323708	24.662014183588028	25.424621421251747
25	25.074244201414547	24.315122388974935	24.8469914991599	25.76364191045062
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158.0
1	158.0
2	493.5
3	829.0
4	829.0
5	829.0
6	2148.0
7	3467.0
8	3467.0
9	3467.0
10	3738.5
11	4010.0
12	4010.0
13	4010.0
14	4628.5
15	5247.0
16	5247.0
17	5247.0
18	8656.5
19	12066.0
20	12066.0
21	12066.0
22	19402.0
23	26738.0
24	26738.0
25	26738.0
26	39692.5
27	52647.0
28	52647.0
29	52647.0
30	64431.0
31	76215.0
32	76215.0
33	76215.0
34	96672.5
35	117130.0
36	117130.0
37	117130.0
38	134871.5
39	152613.0
40	152613.0
41	152613.0
42	176178.0
43	199743.0
44	199743.0
45	199743.0
46	227788.0
47	255833.0
48	255833.0
49	255833.0
50	260243.5
51	264654.0
52	264654.0
53	264654.0
54	247221.0
55	229788.0
56	229788.0
57	229788.0
58	211700.0
59	193612.0
60	193612.0
61	193612.0
62	172616.5
63	151621.0
64	151621.0
65	151621.0
66	126033.0
67	100445.0
68	100445.0
69	100445.0
70	76919.0
71	53393.0
72	53393.0
73	53393.0
74	42062.5
75	30732.0
76	30732.0
77	30732.0
78	25305.0
79	19878.0
80	19878.0
81	19878.0
82	14152.0
83	8426.0
84	8426.0
85	8426.0
86	6250.5
87	4075.0
88	4075.0
89	4075.0
90	2765.5
91	1456.0
92	1456.0
93	1456.0
94	904.5
95	353.0
96	353.0
97	353.0
98	591.0
99	829.0
100	829.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009766231018160102
2	0.0035097392721512868
3	0.005137444441844637
4	0.015005407033110577
5	0.03504652693495995
6	0.04440583166069672
7	0.06500647521462818
8	0.08448807146439548
9	0.09171101315490972
10	0.11516014075580454
11	0.1153636039020162
12	0.12869043997888052
13	0.11658438277928622
14	0.12024671941109628
15	0.11048048839293616
16	0.10798806485184322
17	0.11856814845485002
18	0.12782572160748093
19	0.12787658739403385
20	0.11826295373553249
21	0.12660494273021092
22	0.14883329145383573
23	0.13764281841219395
24	0.13031814514857387
25	0.1274187953150576
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1965958.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.97912862019618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64719652154415	40.6034467570984
2	12.033929045100313	12.269584331929668
3	3.3746992073691104	5.1611767484083195
4	1.456583651262988	2.970214612152434
5	0.8222528202981977	2.095886614215044
6	0.5147198160379596	1.5743980623097713
7	0.35462844464187465	1.265505436424477
8	0.27036647021448856	1.102643764772228
9	0.20454659264392086	0.9384846349695586
>10	1.1132840816317295	10.294939883271605
>50	0.09914934120262137	3.570395384240295
>100	0.09075431623821018	10.07880407019667
>500	0.013292340845434155	4.566729869708686
>1k	0.004597350969097527	3.5077898303028343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3240	0.16480514843145172	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2090	0.10630949389559695	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2075	0.10554650709730319	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2039	0.10371533878139817	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1993	0.10137551259996398	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1990	0.10122291524030523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.017315731058344E-4	0.0	0.0	0.0
6	0.0	1.017315731058344E-4	0.0	0.0	0.0
7	0.0	1.017315731058344E-4	0.0	2.034631462116688E-4	0.0
8	0.0	1.017315731058344E-4	0.0	2.54328932764586E-4	0.0
9	0.0	1.017315731058344E-4	0.0	2.54328932764586E-4	0.0
10	0.0	1.017315731058344E-4	0.0	2.54328932764586E-4	0.0
11	0.0	1.017315731058344E-4	0.0	2.54328932764586E-4	0.0
12	0.0	1.017315731058344E-4	0.0	2.54328932764586E-4	1.017315731058344E-4
13	0.0	1.017315731058344E-4	0.0	2.54328932764586E-4	1.017315731058344E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCC	50	0.0015006107	13.299037	4
AATCGTA	90	5.407128E-7	12.6667185	13
ACGGTAT	430	0.0	12.593074	9
CGTCTTA	180	0.0	12.139247	15
GCGTTAT	160	3.6379788E-12	11.869909	1
CGATAAC	145	9.822543E-11	11.79315	10
CGCAAGA	365	0.0	11.708798	2
AGTTAAA	260	0.0	11.695631	17
GTCTAAT	65	8.053993E-4	11.687295	1
GTTCAAA	500	0.0	11.585031	1
CGGTCCA	375	0.0	11.400045	10
AATCCCC	405	0.0	11.259305	11
CGAACGT	135	4.7348294E-9	11.258444	4
TCGAACG	135	4.7493813E-9	11.256437	3
CGACCAT	340	0.0	11.176516	10
TTAGAAC	145	1.2223609E-9	11.13514	3
GTATAGA	145	1.2259989E-9	11.133156	1
AAGACGG	420	0.0	11.082814	5
ATCCCCG	405	0.0	11.025017	12
CGCAGTT	95	1.35631E-5	11.003127	17
>>END_MODULE