FastQCFastQC Report
Thu 2 Feb 2017
SRR4062563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965958
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21340.1085475885039253No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20550.10452919136624485No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20090.10218936518481066No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19880.1011211836671994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33750.019.9491351
TACCGTC5850.018.051227
CGTCGTA5800.017.06890710
ATACCGT6150.016.812956
AATACCG800.00198899316.4999435
TTAACGG3100.016.32252535
AACGCAG41350.016.2804766
GTCTAAA1906.166374E-1016.2125321
CCTTATA1805.2696123E-915.8892372
TAGGACG1805.2714313E-915.8888334
ACCGTCG6250.015.8399458
AGCGTAT4450.015.82016858
TAACGGC3200.015.81244536
TATATCG850.002980897415.5293585
TAGCGTA4400.015.4999477
CCGTCGT6250.015.4879469
ATAGCGT4550.015.4724746
CGTATAT4600.015.30429510
GCATATA2051.8080755E-915.0262491
TCAACGC45050.014.9433444
Produced by FastQC (version 0.11.5)