##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1965958
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329608262231442	32.0	32.0	32.0	32.0	32.0
2	31.440311034111613	32.0	32.0	32.0	32.0	32.0
3	31.51203281046696	32.0	32.0	32.0	32.0	32.0
4	31.604895425029426	32.0	32.0	32.0	32.0	32.0
5	31.550166381987815	32.0	32.0	32.0	32.0	32.0
6	35.13636506985399	36.0	36.0	36.0	36.0	36.0
7	35.1547362659833	36.0	36.0	36.0	36.0	36.0
8	35.1006888244815	36.0	36.0	36.0	36.0	36.0
9	35.223021041141266	36.0	36.0	36.0	36.0	36.0
10	35.10262731960703	36.0	36.0	36.0	36.0	36.0
11	35.23139863618653	36.0	36.0	36.0	36.0	36.0
12	35.14369482969626	36.0	36.0	36.0	36.0	36.0
13	35.186902772083634	36.0	36.0	36.0	36.0	36.0
14	35.146108919926064	36.0	36.0	36.0	36.0	36.0
15	35.111446429679575	36.0	36.0	36.0	36.0	36.0
16	35.124042324403675	36.0	36.0	36.0	36.0	36.0
17	35.098403933349545	36.0	36.0	36.0	36.0	36.0
18	35.111524254333006	36.0	36.0	36.0	36.0	36.0
19	35.0898386435519	36.0	36.0	36.0	36.0	36.0
20	35.088538005389736	36.0	36.0	36.0	36.0	36.0
21	35.08545909932969	36.0	36.0	36.0	36.0	36.0
22	35.06713419106614	36.0	36.0	36.0	36.0	36.0
23	35.03366297754072	36.0	36.0	36.0	36.0	36.0
24	35.00942390427466	36.0	36.0	36.0	36.0	36.0
25	34.98806892110615	36.0	36.0	36.0	36.0	36.0
26	34.94552986381194	36.0	36.0	36.0	32.0	36.0
27	34.92750608100478	36.0	36.0	36.0	32.0	36.0
28	34.919210379875864	36.0	36.0	36.0	32.0	36.0
29	34.904676498684104	36.0	36.0	36.0	32.0	36.0
30	34.884984826735874	36.0	36.0	36.0	32.0	36.0
31	34.88416080099371	36.0	36.0	36.0	32.0	36.0
32	34.854100647114535	36.0	36.0	36.0	32.0	36.0
33	34.83632458068789	36.0	36.0	36.0	32.0	36.0
34	34.83048773168094	36.0	36.0	36.0	32.0	36.0
35	34.79792752439269	36.0	36.0	36.0	32.0	36.0
36	34.780491241420215	36.0	36.0	36.0	32.0	36.0
37	34.77505470615344	36.0	36.0	36.0	32.0	36.0
38	34.74501642456248	36.0	36.0	36.0	32.0	36.0
39	34.739757410890775	36.0	36.0	36.0	32.0	36.0
40	34.732567531961514	36.0	36.0	36.0	32.0	36.0
41	34.70225406646531	36.0	36.0	36.0	32.0	36.0
42	34.673066260825514	36.0	36.0	36.0	32.0	36.0
43	34.679472297983985	36.0	36.0	36.0	32.0	36.0
44	34.64426249187419	36.0	36.0	36.0	32.0	36.0
45	34.61433051977713	36.0	36.0	36.0	32.0	36.0
46	34.619700929521386	36.0	36.0	36.0	32.0	36.0
47	34.590836630284066	36.0	36.0	36.0	32.0	36.0
48	34.5488591312734	36.0	36.0	36.0	32.0	36.0
49	34.5466413829797	36.0	36.0	36.0	32.0	36.0
50	34.03967429619554	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	13.0
21	39.0
22	146.0
23	434.0
24	1100.0
25	2706.0
26	5764.0
27	10305.0
28	18130.0
29	28034.0
30	40888.0
31	60114.0
32	89247.0
33	148473.0
34	332110.0
35	1228455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08313043018182	17.854951633925747	11.309277312053007	26.752640623839437
2	16.796540187711706	20.517557723307597	35.795241537280624	26.890660551700073
3	18.390128721745157	23.885803577514338	28.663300009257632	29.06076769148288
4	12.75612844024564	15.580503541023532	35.76883929811282	35.894528720618
5	14.80062137644853	36.1310363700547	33.255288261498976	15.813053991997794
6	34.637826311872786	35.21678334483753	16.461210336585538	13.68418000670414
7	30.313786110276062	30.399342406776952	20.796233081394963	18.49063840155202
8	28.354827519204377	32.28044546221231	19.566949039603085	19.79777797898022
9	27.776025734018734	13.935546944543068	18.521301065434763	39.76712625600343
10	16.26525083445323	26.194710161661643	31.22121632303437	26.31882268085076
11	37.844348658516616	20.96702981447213	21.986125848059825	19.202495678951433
12	24.788118566113823	23.561490123390225	28.212301585283107	23.438089725212848
13	29.520620481210685	19.074822554703612	25.21157623916686	26.192980724918847
14	23.915821192517846	19.48912438617712	24.51573227912295	32.07932214218208
15	25.638797980424812	26.597923251666618	21.970052259509103	25.79322650839947
16	26.39593521326498	25.472161663677458	23.059699139045698	25.072203984011864
17	24.59523550350516	25.371650869448892	24.72301035932609	25.310103267719857
18	25.53365839961993	24.413085121859165	25.615654047543234	24.43760243097767
19	25.980616066060414	24.83425383451732	24.917368529744785	24.267761569677482
20	25.8714072223313	24.01684064461194	24.642540684999375	25.469211448057383
21	27.165229369091303	23.926604739267066	24.106873086810605	24.80129280483103
22	26.168209086867574	24.06597699442206	24.426818884228453	25.338995034481915
23	24.907856627659392	23.78529958422306	25.071440997213575	26.23540279090398
24	25.037666114942436	24.87438694010757	24.660852368158427	25.42709457679157
25	25.25816929964933	24.179560295794722	24.883746244833308	25.678524159722638
26	25.0701184867632	24.7449335133304	25.243062161043117	24.941885838863293
27	25.878782761381476	24.45153965649317	24.348383841363855	25.3212937407615
28	25.01197889273321	24.14766744762604	25.332331616443483	25.508022043197258
29	25.045753775004346	24.50210024832677	25.08390311491904	25.368242861749845
30	24.9402581336936	24.733895637648416	25.365852169781856	24.95999405887613
31	25.434114055335872	24.453523422168736	24.309369783077766	25.80299273941763
32	25.09916285088491	24.495385964501786	24.363084053677646	26.042367130935656
33	24.960757045674427	24.02223241798655	24.90958606440219	26.107424471936834
34	25.49942572526982	24.13525619570713	25.240976663794445	25.124341415228606
35	26.21892227606083	23.997002987856302	24.924896666154616	24.859178069928248
36	25.008570885034164	24.62885778841664	24.702714910491473	25.659856416057718
37	26.032804363063704	24.292736670874962	24.535824264811353	25.138634701249973
38	25.087718048910506	24.079761622577898	25.133242927875365	25.699277400636227
39	25.6088888979317	24.15219450262925	24.35408080945778	25.88483578998127
40	25.732594490828394	24.205654444296368	25.094025406443066	24.967725658432176
41	24.70520733403257	24.60759588963752	25.422109729709387	25.265087046620526
42	25.967899619422184	24.66075063658532	24.853430236047767	24.517919507944725
43	25.026831702406664	23.62141001994956	25.209439876131633	26.14231840151214
44	24.80266618106796	24.32600289528057	24.991276517606174	25.8800544060453
45	24.859228935714803	24.446707406770642	25.101146616560477	25.592917040954077
46	24.74237711443039	24.200350465804153	25.248797658135615	25.808474761629846
47	24.939698610041518	24.017857960342997	25.595765525001042	25.44667790461444
48	25.495102133412818	24.848394523179028	24.64905150567815	25.007451837730006
49	24.480177094322464	24.766551472615387	25.091736446048184	25.66153498701396
50	24.349401157094913	24.952771117185616	25.028052481283936	25.669775244435538
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	15.0
2	23.0
3	83.0
4	143.0
5	168.0
6	193.0
7	217.0
8	241.0
9	336.5
10	432.0
11	553.5
12	675.0
13	1266.5
14	1858.0
15	2509.0
16	3160.0
17	3847.5
18	4535.0
19	4788.5
20	5042.0
21	5945.5
22	6849.0
23	7432.0
24	8015.0
25	9845.5
26	11676.0
27	14613.5
28	17551.0
29	20211.0
30	22871.0
31	24303.0
32	25735.0
33	30030.0
34	34325.0
35	40238.5
36	46152.0
37	57505.0
38	68858.0
39	75406.5
40	81955.0
41	91592.0
42	101229.0
43	103936.5
44	106644.0
45	117557.5
46	128471.0
47	136572.5
48	144674.0
49	149117.0
50	153560.0
51	148542.5
52	143525.0
53	142008.5
54	140492.0
55	141660.0
56	142828.0
57	139348.5
58	135869.0
59	124224.0
60	112579.0
61	99670.5
62	86762.0
63	75785.5
64	64809.0
65	55779.5
66	46750.0
67	40837.5
68	34925.0
69	31618.5
70	28312.0
71	22709.0
72	17106.0
73	15374.5
74	13643.0
75	10236.0
76	6829.0
77	6195.0
78	5561.0
79	4898.5
80	4236.0
81	3425.0
82	2614.0
83	2331.5
84	2049.0
85	1704.5
86	1360.0
87	968.0
88	576.0
89	376.5
90	177.0
91	116.5
92	56.0
93	34.5
94	13.0
95	8.0
96	3.0
97	4.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016480514843145173
2	0.003611470845257121
3	6.103894386350064E-4
4	5.08657865529172E-5
5	0.0
6	4.577920789762548E-4
7	5.08657865529172E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.525973596587516E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1965958.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.914765638715934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.38302543136518	43.338319774629966
2	11.812553442848744	12.737421009319942
3	3.2560722367963555	5.266511146488153
4	1.37628398195487	2.968081133576623
5	0.745952794395715	2.0108935043695113
6	0.48813775595168946	1.579069962692643
7	0.31796976162529333	1.200028562475824
8	0.22726340612427995	0.9802282623557498
9	0.18451714722777637	0.8953378874199006
>10	1.0291860496269074	9.972752936013437
>50	0.08415781069239296	3.166877664776501
>100	0.08115138294053148	9.454772246782618
>500	0.010509631917147983	3.899192227131897
>1k	0.0032191665331804628	2.5305136819671974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2134	0.1085475885039253	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2055	0.10452919136624485	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2009	0.10218936518481066	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1988	0.1011211836671994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.08657865529172E-5
2	0.0	0.0	0.0	0.0	5.08657865529172E-5
3	0.0	0.0	0.0	0.0	5.08657865529172E-5
4	0.0	0.0	0.0	5.08657865529172E-5	5.08657865529172E-5
5	0.0	0.0	0.0	5.08657865529172E-5	5.08657865529172E-5
6	0.0	0.0	0.0	5.08657865529172E-5	5.08657865529172E-5
7	0.0	0.0	0.0	1.017315731058344E-4	5.08657865529172E-5
8	0.0	0.0	0.0	1.017315731058344E-4	5.08657865529172E-5
9	0.0	0.0	0.0	1.017315731058344E-4	5.08657865529172E-5
10	0.0	0.0	0.0	1.017315731058344E-4	5.08657865529172E-5
11	0.0	0.0	0.0	1.017315731058344E-4	5.08657865529172E-5
12	0.0	0.0	0.0	1.017315731058344E-4	1.017315731058344E-4
13	0.0	0.0	0.0	1.017315731058344E-4	1.017315731058344E-4
14	0.0	0.0	0.0	1.017315731058344E-4	1.017315731058344E-4
15	0.0	0.0	0.0	1.017315731058344E-4	1.5259735965875162E-4
16	0.0	0.0	0.0	1.017315731058344E-4	1.5259735965875162E-4
17	0.0	0.0	0.0	1.017315731058344E-4	1.5259735965875162E-4
18	0.0	0.0	0.0	1.5259735965875162E-4	2.034631462116688E-4
19	0.0	0.0	0.0	2.034631462116688E-4	2.034631462116688E-4
20	0.0	1.017315731058344E-4	0.0	2.54328932764586E-4	2.034631462116688E-4
21	0.0	1.017315731058344E-4	0.0	3.0519471931750325E-4	2.034631462116688E-4
22	0.0	1.017315731058344E-4	0.0	3.560605058704204E-4	2.54328932764586E-4
23	0.0	1.017315731058344E-4	0.0	6.612552251879237E-4	2.54328932764586E-4
24	0.0	1.017315731058344E-4	0.0	0.0010681815176112612	2.54328932764586E-4
25	0.0	1.017315731058344E-4	0.0	0.0013225104503758473	2.54328932764586E-4
26	0.0	1.017315731058344E-4	0.0	0.001983765675563771	2.54328932764586E-4
27	0.0	1.017315731058344E-4	0.0	0.003001081406622115	3.0519471931750325E-4
28	0.0	1.017315731058344E-4	0.0	0.0076298679829375805	3.0519471931750325E-4
29	0.0	1.017315731058344E-4	0.0	0.021160167206013557	3.0519471931750325E-4
30	0.0	1.017315731058344E-4	0.0	0.04506708688588464	3.0519471931750325E-4
31	0.0	1.017315731058344E-4	0.0	0.10325754670242192	3.0519471931750325E-4
32	0.0	1.017315731058344E-4	0.0	0.17197722433541307	3.0519471931750325E-4
33	0.0	1.017315731058344E-4	0.0	0.2370854311231471	3.560605058704204E-4
34	0.0	1.017315731058344E-4	0.0	0.31679211865156837	4.069262924233376E-4
35	0.0	1.017315731058344E-4	0.0	0.4032639557915276	4.069262924233376E-4
36	0.0	1.017315731058344E-4	0.0	0.5272238776209868	4.069262924233376E-4
37	0.0	1.017315731058344E-4	0.0	0.715732482586098	4.069262924233376E-4
38	0.0	1.017315731058344E-4	0.0	0.9662464813592152	4.069262924233376E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3375	0.0	19.949135	1
TACCGTC	585	0.0	18.05122	7
CGTCGTA	580	0.0	17.068907	10
ATACCGT	615	0.0	16.81295	6
AATACCG	80	0.001988993	16.499943	5
TTAACGG	310	0.0	16.322525	35
AACGCAG	4135	0.0	16.280476	6
GTCTAAA	190	6.166374E-10	16.212532	1
CCTTATA	180	5.2696123E-9	15.889237	2
TAGGACG	180	5.2714313E-9	15.888833	4
ACCGTCG	625	0.0	15.839945	8
AGCGTAT	445	0.0	15.8201685	8
TAACGGC	320	0.0	15.812445	36
TATATCG	85	0.0029808974	15.529358	5
TAGCGTA	440	0.0	15.499947	7
CCGTCGT	625	0.0	15.487946	9
ATAGCGT	455	0.0	15.472474	6
CGTATAT	460	0.0	15.304295	10
GCATATA	205	1.8080755E-9	15.026249	1
TCAACGC	4505	0.0	14.943344	4
>>END_MODULE