FastQCFastQC Report
Thu 2 Feb 2017
SRR4062562_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062562_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007883
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60630.30195982534838933No Hit
GTCCTAAAGTGTGTATTTCTCATTT60210.29986807000208676No Hit
CTGTAGGACGTGGAATATGGCAAGA54640.27212740981421724No Hit
CTTTAGGACGTGAAATATGGCGAGG49090.24448635702379073No Hit
GTCCTACAGTGTGCATTTCTCATTT40140.19991204666805784No Hit
GTATCAACGCAGAGTACTTTTTTTT37750.18800896267362194No Hit
CTGTAGGACCTGGAATATGGCGAGA29680.1478173778053801No Hit
CTGAAGGACCTGGAATATGGCGAGA26140.1301868684579729No Hit
GTCCTTCAGTGTGCATTTCTCATTT24800.12351317282929333No Hit
TATCAACGCAGAGTACTTTTTTTTT22850.11380145157860294No Hit
ATTTAGAAATGTCCACTGTAGGACG22420.1116598925335789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTT704.4293847E-714.93108212
GGCGTGC851.7165803E-814.5300418
AGGCGTG1006.6029315E-1014.2506187
AGCGGAC400.005278291614.2506178
AAGGCGT1006.6029315E-1014.2499066
TATACCG400.00528703514.2470635
GACGTGT551.9598776E-413.8187817
TGCGCCA903.6327037E-813.72452912
AAAGGCG1051.375156E-913.5686315
TAGGACC11050.013.4929364
TCGCATT852.6866292E-713.41736718
GCGCCAC852.693996E-713.41435313
ATTTCGC655.4403303E-513.157371515
TCCAACG2500.012.92539618
TTCGCAT905.3802614E-712.67195917
CATTTCG905.3933036E-712.66942914
GTGCGCC751.47843E-512.66721511
GTGCGTT604.0914505E-412.66721411
CACTCGG550.003063972612.09354514
GTACCGC550.00306511812.09294112