##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062562_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2007883
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2506251609282	32.0	32.0	32.0	32.0	32.0
2	30.951286504243523	32.0	32.0	32.0	32.0	32.0
3	30.934500167589444	32.0	32.0	32.0	32.0	32.0
4	31.008346103831748	32.0	32.0	32.0	32.0	32.0
5	30.86954419156893	32.0	32.0	32.0	32.0	32.0
6	34.536132832440934	36.0	36.0	36.0	32.0	36.0
7	34.418316206671406	36.0	36.0	36.0	32.0	36.0
8	34.4349302225279	36.0	36.0	36.0	32.0	36.0
9	34.546154332697675	36.0	36.0	36.0	32.0	36.0
10	34.266083232937376	36.0	36.0	36.0	32.0	36.0
11	34.545815667546364	36.0	36.0	36.0	32.0	36.0
12	34.35317396481767	36.0	36.0	36.0	32.0	36.0
13	34.43579581081168	36.0	36.0	36.0	32.0	36.0
14	34.367793342540374	36.0	36.0	36.0	32.0	36.0
15	34.32847730669566	36.0	36.0	36.0	32.0	36.0
16	34.33755502686162	36.0	36.0	36.0	32.0	36.0
17	34.29415907201764	36.0	36.0	36.0	32.0	36.0
18	34.31469214092654	36.0	36.0	36.0	32.0	36.0
19	34.270940587673685	36.0	36.0	36.0	32.0	36.0
20	34.26576946963543	36.0	36.0	36.0	32.0	36.0
21	34.236560596409255	36.0	36.0	36.0	32.0	36.0
22	34.19331504873541	36.0	36.0	36.0	32.0	36.0
23	34.16818061610164	36.0	36.0	36.0	32.0	36.0
24	34.14247294289558	36.0	36.0	36.0	32.0	36.0
25	33.701496053305895	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	12.0
4	89.0
5	174.0
6	353.0
7	94.0
8	291.0
9	367.0
10	264.0
11	74.0
12	158.0
13	109.0
14	283.0
15	367.0
16	578.0
17	770.0
18	933.0
19	1329.0
20	2020.0
21	2926.0
22	4412.0
23	6754.0
24	9800.0
25	14355.0
26	20206.0
27	26451.0
28	36546.0
29	49328.0
30	64103.0
31	88945.0
32	127553.0
33	186363.0
34	432540.0
35	929335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4985256849588	20.763336574169998	12.255393203806124	24.482744537065077
2	12.777608716332214	22.401145124281747	44.36480017850232	20.456445980883718
3	17.78540587842997	28.225986798360974	30.471002564522724	23.517604758686332
4	10.449307573603615	17.96587729094461	41.39674772287801	30.18806741257376
5	10.745249770073825	40.821735720911974	36.700153350544994	11.732861158469204
6	27.84088134613564	40.34779489070372	20.29148998719437	11.519833775966278
7	25.77407292410604	33.51781737027267	24.002876655185627	16.705233050435663
8	23.33741098213511	39.653190486017856	21.95694242140556	15.052456110441472
9	26.84085972510054	15.533150505733845	21.884224364461385	35.74176540470423
10	13.247198395578467	31.272258007896454	36.40928905018205	19.07125454634303
11	32.60109680900185	23.629063543716285	26.568098794587158	17.2017408526947
12	22.259819159011105	26.652327189434207	34.11696764441422	16.970886007140482
13	28.03692833043615	24.169167417141136	27.185027610479317	20.608876641943393
14	19.26083912457214	24.408675289030025	29.75433445301716	26.576151133380677
15	20.84755228018389	34.84163866090928	24.64548201481895	19.665327044087878
16	19.294358156116775	30.440577397721324	30.96208022736905	19.302984218792847
17	18.923388011658727	31.14181208907107	30.188918226856458	19.745881672413752
18	18.826414281354168	29.698611800988235	33.33727311354347	18.13770080411413
19	21.817549830043248	28.327721302842185	29.468595901887877	20.38613296522669
20	22.286996812631394	29.395397264208523	30.302490854765296	18.01511506839479
21	21.57901981005803	28.587343984073758	28.204647414479233	21.628988791388977
22	21.232089904005626	31.190764189035843	29.074897558615426	18.502248348343105
23	19.974573933843352	30.707186778844598	29.77059184680122	19.547647440510836
24	21.01667916789684	28.996586765187	31.059140128150908	18.92759393876525
25	20.359528999655392	30.102549448203874	30.41010303818263	19.127818513958104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	308.0
1	308.0
2	733.5
3	1159.0
4	1159.0
5	1159.0
6	2709.5
7	4260.0
8	4260.0
9	4260.0
10	5723.5
11	7187.0
12	7187.0
13	7187.0
14	10500.0
15	13813.0
16	13813.0
17	13813.0
18	23678.0
19	33543.0
20	33543.0
21	33543.0
22	53039.0
23	72535.0
24	72535.0
25	72535.0
26	104336.0
27	136137.0
28	136137.0
29	136137.0
30	176712.5
31	217288.0
32	217288.0
33	217288.0
34	239279.0
35	261270.0
36	261270.0
37	261270.0
38	271517.0
39	281764.0
40	281764.0
41	281764.0
42	279609.0
43	277454.0
44	277454.0
45	277454.0
46	270201.5
47	262949.0
48	262949.0
49	262949.0
50	232116.5
51	201284.0
52	201284.0
53	201284.0
54	159126.5
55	116969.0
56	116969.0
57	116969.0
58	90938.0
59	64907.0
60	64907.0
61	64907.0
62	48141.5
63	31376.0
64	31376.0
65	31376.0
66	22805.5
67	14235.0
68	14235.0
69	14235.0
70	9759.0
71	5283.0
72	5283.0
73	5283.0
74	3657.0
75	2031.0
76	2031.0
77	2031.0
78	1390.5
79	750.0
80	750.0
81	750.0
82	528.0
83	306.0
84	306.0
85	306.0
86	223.5
87	141.0
88	141.0
89	141.0
90	113.5
91	86.0
92	86.0
93	86.0
94	85.0
95	84.0
96	84.0
97	84.0
98	424.0
99	764.0
100	764.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008516432481374661
2	0.0032870441156182904
3	0.005079977269591904
4	0.015190128110054223
5	0.035709251983307794
6	0.04746292488157926
7	0.0690279264279841
8	0.09089175016671788
9	0.09891014566087765
10	0.12077396939961142
11	0.12097318419449739
12	0.134270771753135
13	0.12376219132290078
14	0.12724845023340503
15	0.1166900661044493
16	0.11619202911723442
17	0.12540571338070994
18	0.13422096805441353
19	0.13282646449021182
20	0.12286572474591398
21	0.13143196092601014
22	0.15329578466474392
23	0.1421397561511303
24	0.13681076038793097
25	0.13466920134290694
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2007883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.03215296333887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.81121936808759	59.23733221388451
2	10.45067585275864	14.428653080758128
3	1.974137016752279	4.088367855330985
4	0.593003910243473	1.6374534655914204
5	0.2659491783897441	0.9179522181537556
6	0.1578282720836545	0.6537135252250984
7	0.10222121105347765	0.4939585194278991
8	0.07951669111877151	0.43913667075596835
9	0.06558918586232895	0.40749864400702407
>10	0.4169298421694696	5.597690702617956
>50	0.0436871371658279	2.124990118075466
>100	0.03351373274184139	4.69899860019313
>500	0.0031180995902555723	1.511699630820795
>1k	0.0023929601506612387	2.8841380817037163
>5k	2.175418318782944E-4	0.8784166734541935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	6063	0.30195982534838933	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6021	0.29986807000208676	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5464	0.27212740981421724	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4909	0.24448635702379073	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4014	0.19991204666805784	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3775	0.18800896267362194	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2968	0.1478173778053801	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2614	0.1301868684579729	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2480	0.12351317282929333	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2285	0.11380145157860294	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2242	0.1116598925335789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.980369872148925E-5
2	0.0	0.0	0.0	0.0	4.980369872148925E-5
3	0.0	0.0	0.0	0.0	4.980369872148925E-5
4	0.0	0.0	0.0	0.0	4.980369872148925E-5
5	0.0	0.0	0.0	4.980369872148925E-5	4.980369872148925E-5
6	0.0	0.0	0.0	4.980369872148925E-5	4.980369872148925E-5
7	0.0	0.0	0.0	4.980369872148925E-5	4.980369872148925E-5
8	0.0	0.0	0.0	9.96073974429785E-5	4.980369872148925E-5
9	0.0	0.0	0.0	1.4941109616446776E-4	4.980369872148925E-5
10	0.0	0.0	0.0	1.99214794885957E-4	4.980369872148925E-5
11	0.0	0.0	0.0	1.99214794885957E-4	4.980369872148925E-5
12	0.0	0.0	0.0	2.4901849360744625E-4	4.980369872148925E-5
13	0.0	0.0	0.0	2.4901849360744625E-4	4.980369872148925E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTTT	70	4.4293847E-7	14.931082	12
GGCGTGC	85	1.7165803E-8	14.530041	8
AGGCGTG	100	6.6029315E-10	14.250618	7
AGCGGAC	40	0.0052782916	14.250617	8
AAGGCGT	100	6.6029315E-10	14.249906	6
TATACCG	40	0.005287035	14.247063	5
GACGTGT	55	1.9598776E-4	13.818781	7
TGCGCCA	90	3.6327037E-8	13.724529	12
AAAGGCG	105	1.375156E-9	13.568631	5
TAGGACC	1105	0.0	13.492936	4
TCGCATT	85	2.6866292E-7	13.417367	18
GCGCCAC	85	2.693996E-7	13.414353	13
ATTTCGC	65	5.4403303E-5	13.1573715	15
TCCAACG	250	0.0	12.925396	18
TTCGCAT	90	5.3802614E-7	12.671959	17
CATTTCG	90	5.3933036E-7	12.669429	14
GTGCGCC	75	1.47843E-5	12.667215	11
GTGCGTT	60	4.0914505E-4	12.667214	11
CACTCGG	55	0.0030639726	12.093545	14
GTACCGC	55	0.003065118	12.092941	12
>>END_MODULE