FastQCFastQC Report
Thu 2 Feb 2017
SRR4062562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007883
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA31210.15543734370976794No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC24910.12406101351522972No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA23950.11927985843796676No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA21720.10817363362307467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGC407.0341135E-427.4997658
CGGTAAA407.0341135E-427.49976539
GCGTAAC508.344607E-526.39977638
TAGGACC14300.024.461334
GTATCAA46950.022.873521
CGGTAGG703.2095166E-522.0063881
CGTAACT602.8738583E-421.99981539
TGTAGGA39550.021.6393262
GTCGTAA1057.7532604E-820.95220430
GTAGGAC39700.020.7811973
GTCCTAC38500.020.4630851
GGTCGTA1201.43299985E-820.16649629
CTGTAGG40050.020.055761
ACTGTCG550.00448484719.999838
GTCCTAA23150.019.582361
TCCTACA42900.019.3341312
CGTAAAC1051.7940365E-618.85698332
ATTGCGA708.1263424E-418.85698317
TAGGACG38800.018.6544824
GCGAAAA1303.5912308E-818.61522720
Produced by FastQC (version 0.11.5)