##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2007883
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.337145142421146	32.0	32.0	32.0	32.0	32.0
2	31.495726095594215	32.0	32.0	32.0	32.0	32.0
3	31.56680145207664	32.0	32.0	32.0	32.0	32.0
4	31.648619964410276	32.0	32.0	32.0	32.0	32.0
5	31.61511353002142	32.0	32.0	32.0	32.0	32.0
6	35.21588409284804	36.0	36.0	36.0	36.0	36.0
7	35.225020581378494	36.0	36.0	36.0	36.0	36.0
8	35.18104789970332	36.0	36.0	36.0	36.0	36.0
9	35.300024453616075	36.0	36.0	36.0	36.0	36.0
10	35.21202679638206	36.0	36.0	36.0	36.0	36.0
11	35.30974862579144	36.0	36.0	36.0	36.0	36.0
12	35.2476802682228	36.0	36.0	36.0	36.0	36.0
13	35.2763353243192	36.0	36.0	36.0	36.0	36.0
14	35.25186875928528	36.0	36.0	36.0	36.0	36.0
15	35.22229631905843	36.0	36.0	36.0	36.0	36.0
16	35.24424978945486	36.0	36.0	36.0	36.0	36.0
17	35.236941594704476	36.0	36.0	36.0	36.0	36.0
18	35.2398346915632	36.0	36.0	36.0	36.0	36.0
19	35.21958102140414	36.0	36.0	36.0	36.0	36.0
20	35.21441637784672	36.0	36.0	36.0	36.0	36.0
21	35.19234736286925	36.0	36.0	36.0	36.0	36.0
22	35.18023809156211	36.0	36.0	36.0	36.0	36.0
23	35.15085241520547	36.0	36.0	36.0	36.0	36.0
24	35.142480413450386	36.0	36.0	36.0	36.0	36.0
25	35.122472773563004	36.0	36.0	36.0	36.0	36.0
26	35.085994054434444	36.0	36.0	36.0	36.0	36.0
27	35.053419945285654	36.0	36.0	36.0	36.0	36.0
28	35.03682535287166	36.0	36.0	36.0	36.0	36.0
29	35.012394646500816	36.0	36.0	36.0	36.0	36.0
30	34.99333925333299	36.0	36.0	36.0	36.0	36.0
31	34.98163538413344	36.0	36.0	36.0	36.0	36.0
32	34.96093995516671	36.0	36.0	36.0	36.0	36.0
33	34.96428128531394	36.0	36.0	36.0	36.0	36.0
34	34.94415511262359	36.0	36.0	36.0	36.0	36.0
35	34.92808096886123	36.0	36.0	36.0	36.0	36.0
36	34.88393596638848	36.0	36.0	36.0	36.0	36.0
37	34.85774469926783	36.0	36.0	36.0	36.0	36.0
38	34.830519507361736	36.0	36.0	36.0	36.0	36.0
39	34.767007340567154	36.0	36.0	36.0	36.0	36.0
40	34.7870593057464	36.0	36.0	36.0	36.0	36.0
41	34.76079084289274	36.0	36.0	36.0	36.0	36.0
42	34.76129336221284	36.0	36.0	36.0	36.0	36.0
43	34.736758068074685	36.0	36.0	36.0	36.0	36.0
44	34.69572430266106	36.0	36.0	36.0	36.0	36.0
45	34.670255189171876	36.0	36.0	36.0	36.0	36.0
46	34.610130171927345	36.0	36.0	36.0	32.0	36.0
47	34.60143942650045	36.0	36.0	36.0	32.0	36.0
48	34.56436306298723	36.0	36.0	36.0	32.0	36.0
49	34.525126215023484	36.0	36.0	36.0	32.0	36.0
50	34.07406158625776	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	44.0
22	140.0
23	411.0
24	1104.0
25	2528.0
26	5207.0
27	10041.0
28	17515.0
29	27162.0
30	39772.0
31	56572.0
32	83537.0
33	134752.0
34	297909.0
35	1331184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.992928715313546	21.374546961579156	12.675063609682619	24.95746071342468
2	12.42143713451676	23.0067233227429	43.87801096431046	20.693828578429883
3	17.348401388935926	28.47582610012212	30.76497060563742	23.41080190530453
4	10.30630347062919	18.606459157379085	41.368399872900646	29.718837499091077
5	10.558140368806534	41.11028436930059	36.582030218907285	11.749545042985595
6	28.054065172448656	40.60321544241095	19.914058309464792	11.428661075675603
7	25.724233657273516	33.61862580501534	23.989818121691474	16.667322416019676
8	23.319436441266745	39.95526631780836	21.72706278204457	14.998234458880324
9	26.736119584657075	15.423358831166956	21.81362161042252	36.02689997375345
10	13.215162437253566	31.4845038281613	36.311328897151874	18.989004837433257
11	32.6894047113303	23.552567554982037	26.599109609474258	17.15891812421341
12	22.339100435632954	26.74837129454256	33.943710863631	16.968817406193487
13	28.084604531240114	24.061013515229725	27.301939405831916	20.552442547698245
14	19.325130000104586	24.441563577160622	29.673342520455627	26.559963902279165
15	20.877660700349573	34.882261566037464	24.554568169559683	19.685509564053284
16	19.382304646236857	30.469454644518628	30.735605610486267	19.412635098758244
17	19.009125531716737	31.277569459973513	29.995074414196445	19.718230594113304
18	18.900254646311563	29.756614304717953	33.206566318854236	18.13656473011625
19	21.976280490446904	28.34627316432282	29.43064909658581	20.24679724864447
20	22.495683264413316	29.37850462402441	30.165004634234165	17.96080747732811
21	21.67188028386116	28.47655964017824	28.2994078838259	21.552152192134702
22	21.31568423060507	31.106941988153693	29.055079404527056	18.522294376714182
23	19.940653912603473	30.819724057626864	29.685395015546224	19.55422701422344
24	21.083150761274435	29.06897463647035	30.796913963612422	19.05096063864279
25	20.509959992688817	30.010513560800106	30.220436150911183	19.259090295599893
26	18.59914148384144	30.92421221754455	31.045932457219866	19.430713841394144
27	19.623454155446307	30.663888284327324	30.005134761338187	19.70752279888818
28	18.80966171833717	31.02456667046835	29.343143997932152	20.822627613262327
29	20.177370892626712	29.599931868540146	29.114096787512022	21.10860045132112
30	19.383499935006174	29.880774925630625	30.197377038403133	20.538348100960064
31	21.118162761475645	28.930968587313107	29.702577291605138	20.248291359606114
32	19.508357807700946	30.614433211496884	30.08691243463887	19.790296546163297
33	19.127160297686668	30.629922161799268	30.927349850564	19.31556768995006
34	19.65966144441683	30.563633438800963	31.061969248208186	18.714735868574014
35	19.434498922496978	30.856927420571818	30.431055992804364	19.277517664126844
36	18.79103513501534	30.237668230668817	31.381858405096313	19.58943822921953
37	19.209983848660507	29.807613292208757	29.38129363115281	21.601109227977926
38	19.508308004002224	31.21551405136654	29.40634489160972	19.869833053021516
39	20.817896261883785	29.055428030418106	29.985163478150866	20.14151222954724
40	21.57077877545654	28.863285360750602	30.436086166375233	19.12984969741763
41	21.09654795623052	28.11807261678096	31.247936259234226	19.537443167754294
42	19.844781792564607	30.447989250369666	30.535842974914374	19.17138598215135
43	18.736201262722975	30.36586295117793	31.073971939600064	19.823963846499023
44	18.563282820761966	30.219191058443144	30.657563214589693	20.559962906205193
45	18.829732608921933	30.367307258440857	30.345742256894447	20.45721787574276
46	19.245672052114667	28.8362850369544	30.595354304042072	21.322688606888857
47	20.681245212617075	28.833716323967245	30.22194531351942	20.263093149896257
48	18.63758993925443	29.518801643322846	30.362277084869987	21.481331332552745
49	20.053110664316595	28.86766808623809	30.23348471997621	20.845736529469097
50	18.694026840222683	30.844242838971613	29.63983939295238	20.821890927853328
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	32.5
2	33.0
3	103.0
4	173.0
5	233.5
6	294.0
7	314.5
8	335.0
9	475.5
10	616.0
11	930.0
12	1244.0
13	2014.0
14	2784.0
15	4002.5
16	5221.0
17	6290.5
18	7360.0
19	8867.0
20	10374.0
21	12855.5
22	15337.0
23	19097.5
24	22858.0
25	29029.0
26	35200.0
27	45954.5
28	56709.0
29	69471.0
30	82233.0
31	96960.5
32	111688.0
33	132362.0
34	153036.0
35	173472.0
36	193908.0
37	198003.0
38	202098.0
39	194742.0
40	187386.0
41	177483.0
42	167580.0
43	158743.5
44	149907.0
45	141654.5
46	133402.0
47	125421.0
48	117440.0
49	107350.5
50	97261.0
51	87495.0
52	77729.0
53	67873.0
54	58017.0
55	49719.0
56	41421.0
57	35057.5
58	28694.0
59	23512.0
60	18330.0
61	15050.0
62	11770.0
63	9397.5
64	7025.0
65	5585.0
66	4145.0
67	3349.0
68	2553.0
69	2048.5
70	1544.0
71	1214.5
72	885.0
73	694.0
74	503.0
75	428.0
76	353.0
77	272.0
78	191.0
79	143.0
80	95.0
81	74.5
82	54.0
83	43.5
84	33.0
85	20.5
86	8.0
87	9.0
88	10.0
89	9.5
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016285809481926986
2	0.004781155077262968
3	7.470554808223388E-4
4	2.4901849360744625E-4
5	4.9803698721489254E-5
6	4.980369872148925E-4
7	9.960739744297851E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.4941109616446776E-4
47	4.9803698721489254E-5
48	0.0
49	0.0
50	4.9803698721489254E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2007883.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.8632178627096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.75677428913168	67.55161617540584
2	10.1623971675805	15.825538893334068
3	1.8015532888275172	4.208242086577741
4	0.4869564787457948	1.5166399357696696
5	0.2056171707869493	0.80050072826491
6	0.11727154403310726	0.5478683869287704
7	0.07245786530961533	0.39492617867286134
8	0.05189966084730179	0.32328596796433595
9	0.043380982164156645	0.3040004578811444
>10	0.2628546886934691	3.8351633303643196
>50	0.02310132949202098	1.2578553807139559
>100	0.013930905613608153	2.009247353586638
>500	0.0013534715807694987	0.7310753269327478
>1k	4.511571935898171E-4	0.6940397976029399
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3121	0.15543734370976794	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2491	0.12406101351522972	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2395	0.11927985843796676	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	2172	0.10817363362307467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.980369872148925E-5	0.0
4	0.0	0.0	0.0	9.96073974429785E-5	0.0
5	0.0	0.0	0.0	1.4941109616446776E-4	0.0
6	0.0	0.0	0.0	1.4941109616446776E-4	0.0
7	0.0	0.0	0.0	1.99214794885957E-4	0.0
8	0.0	0.0	0.0	1.99214794885957E-4	0.0
9	0.0	0.0	0.0	1.99214794885957E-4	0.0
10	0.0	0.0	0.0	1.99214794885957E-4	0.0
11	0.0	0.0	0.0	1.99214794885957E-4	0.0
12	0.0	0.0	0.0	2.4901849360744625E-4	0.0
13	0.0	0.0	0.0	2.4901849360744625E-4	0.0
14	0.0	0.0	0.0	2.4901849360744625E-4	0.0
15	0.0	0.0	0.0	3.486258910504248E-4	0.0
16	0.0	0.0	0.0	5.478406859363818E-4	0.0
17	0.0	0.0	0.0	5.478406859363818E-4	0.0
18	0.0	0.0	0.0	6.474480833793603E-4	0.0
19	0.0	0.0	0.0	7.96859179543828E-4	0.0
20	0.0	0.0	0.0	0.0012948961667587206	0.0
21	0.0	0.0	0.0	0.0014941109616446774	0.0
22	0.0	0.0	0.0	0.0020917553463025485	0.0
23	0.0	0.0	0.0	0.0026395960322389304	0.0
24	0.0	0.0	0.0	0.0034862589105042474	0.0
25	0.0	0.0	0.0	0.00393449219899765	0.0
26	0.0	0.0	0.0	0.00532899576319935	0.0
27	0.0	0.0	0.0	0.006474480833793602	0.0
28	0.0	0.0	0.0	0.011156028513613593	0.0
29	0.0	0.0	0.0	0.018975209212887405	0.0
30	0.0	0.0	0.0	0.03805002582321779	0.0
31	0.0	0.0	0.0	0.08884979851913682	0.0
32	0.0	0.0	0.0	0.16255927262694092	0.0
33	0.0	0.0	0.0	0.23791226879255414	0.0
34	0.0	0.0	0.0	0.32970048553625886	0.0
35	0.0	0.0	0.0	0.4359815786079169	0.0
36	0.0	0.0	0.0	0.5799142679130208	0.0
37	0.0	0.0	0.0	0.7922274355627295	0.0
38	0.0	0.0	0.0	1.0724728482685495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGCGC	40	7.0341135E-4	27.499765	8
CGGTAAA	40	7.0341135E-4	27.499765	39
GCGTAAC	50	8.344607E-5	26.399776	38
TAGGACC	1430	0.0	24.46133	4
GTATCAA	4695	0.0	22.87352	1
CGGTAGG	70	3.2095166E-5	22.006388	1
CGTAACT	60	2.8738583E-4	21.999815	39
TGTAGGA	3955	0.0	21.639326	2
GTCGTAA	105	7.7532604E-8	20.952204	30
GTAGGAC	3970	0.0	20.781197	3
GTCCTAC	3850	0.0	20.463085	1
GGTCGTA	120	1.43299985E-8	20.166496	29
CTGTAGG	4005	0.0	20.05576	1
ACTGTCG	55	0.004484847	19.99983	8
GTCCTAA	2315	0.0	19.58236	1
TCCTACA	4290	0.0	19.334131	2
CGTAAAC	105	1.7940365E-6	18.856983	32
ATTGCGA	70	8.1263424E-4	18.856983	17
TAGGACG	3880	0.0	18.654482	4
GCGAAAA	130	3.5912308E-8	18.615227	20
>>END_MODULE