FastQCFastQC Report
Thu 2 Feb 2017
SRR4062561_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062561_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123828
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG45740.4070017831910221No Hit
TCCATGTACTCTGCGTTGATACCAC41110.3658033079795129No Hit
GTATCAACGCAGAGTACTTTTTTTT21320.1897087454663881No Hit
GAGTACATGGAAGCAGTGGTATCAA20280.18045466032168622No Hit
CATGTACTCTGCGTTGATACCACTG19890.17698437839242304No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16660.14824332549108937No Hit
TATCAACGCAGAGTACTTTTTTTTT16610.14779841755144027No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13640.12137088593628206No Hit
CCCATGTACTCTGCGTTGATACCAC13410.11932430941389609No Hit
GCGCAAGACGGACCAGAGCGAAAGC13390.11914634623803642No Hit
GCTTCCATGTACTCTGCGTTGATAC13060.11620995383635217No Hit
GTCTTGCGCCGGTCCAAGAATTTCA12770.11362948778638725No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12330.10971429791747493No Hit
GAATAGGACCGCGGTTCTATTTTGT11890.10579910804856259No Hit
GAACTACGACGGTATCTGATCGTCT11870.10562114487270294No Hit
GTATCTGATCGTCTTCGAACCTCCG11500.10232882611929939No Hit
GAATAACGCCGCCGCATCGCCAGTC11260.1001932680089836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATT508.699356E-515.20228213
GTATTAC508.755737E-515.1907731
GCGTTAT1300.014.6065131
CGTTATT1350.014.0680362
CGGACAT1301.8189894E-1213.8829875
CGAACGA551.9568948E-413.82025616
AATACTC551.9613383E-413.8165625
GTAAGAC551.9643056E-413.81413
GGACGCT500.001498909213.3002187
GACGCTC500.001498909213.3002188
CTTATGC500.001502436213.2960713
AATCGTA604.0852753E-412.66856913
CTAATTG604.1006604E-412.6629243
AAGACGG2050.012.5107285
CCGACCA2000.012.3502039
CTAAGAC853.9535917E-612.2904863
ACGGTAT2250.012.2446459
ATTGTCG550.003067155412.0911076
TAGAACC951.042059E-611.9969894
ATCGCCA1750.011.9446516
Produced by FastQC (version 0.11.5)