##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062561_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1123828
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05859259602003	32.0	32.0	32.0	32.0	32.0
2	30.800712386592966	32.0	32.0	32.0	32.0	32.0
3	30.84431781375798	32.0	32.0	32.0	32.0	32.0
4	30.774659467462993	32.0	32.0	32.0	32.0	32.0
5	30.763849984161276	32.0	32.0	32.0	32.0	32.0
6	34.25851820741252	36.0	36.0	36.0	32.0	36.0
7	34.17104930647751	36.0	36.0	36.0	32.0	36.0
8	34.153384681641676	36.0	36.0	36.0	32.0	36.0
9	34.24939136593856	36.0	36.0	36.0	32.0	36.0
10	34.02916015618048	36.0	36.0	36.0	32.0	36.0
11	34.28297479685504	36.0	36.0	36.0	32.0	36.0
12	34.09829351110668	36.0	36.0	36.0	32.0	36.0
13	34.19820203803429	36.0	36.0	36.0	32.0	36.0
14	34.11519111465456	36.0	36.0	36.0	32.0	36.0
15	34.082285723438105	36.0	36.0	36.0	32.0	36.0
16	34.08677573436505	36.0	36.0	36.0	32.0	36.0
17	34.008839430944946	36.0	36.0	36.0	32.0	36.0
18	34.04726168061305	36.0	36.0	36.0	32.0	36.0
19	34.00583986161583	36.0	36.0	36.0	32.0	36.0
20	33.989796481312084	36.0	36.0	36.0	32.0	36.0
21	33.93766483839164	36.0	36.0	36.0	32.0	36.0
22	33.891359709848835	36.0	36.0	36.0	32.0	36.0
23	33.87513302747396	36.0	36.0	36.0	32.0	36.0
24	33.8573020070687	36.0	36.0	36.0	32.0	36.0
25	33.25482992059283	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	39.0
5	99.0
6	211.0
7	47.0
8	152.0
9	204.0
10	136.0
11	52.0
12	95.0
13	67.0
14	377.0
15	561.0
16	900.0
17	1156.0
18	1507.0
19	1965.0
20	2480.0
21	2986.0
22	3740.0
23	4769.0
24	6266.0
25	8333.0
26	11619.0
27	15222.0
28	20887.0
29	28862.0
30	38796.0
31	55148.0
32	83207.0
33	124542.0
34	280422.0
35	428975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.456017826377916	17.596607008101607	11.181976421340394	25.765398744180086
2	16.439533685534055	20.137374298245845	37.270186360275176	26.152905655944924
3	19.09651033657953	24.08229042086679	28.615489207813656	28.205710034740015
4	12.397256129034554	15.396629609457598	36.016866191793305	36.189248069714544
5	14.20323808726938	36.84403542331106	34.007126319940255	14.945600169479306
6	34.05422439670552	35.32490839751738	16.98838811678115	13.632479088995952
7	29.575459475920084	30.755258117682466	21.27323869418996	18.396043712207486
8	27.973320912304388	32.985758269083306	20.007267370663513	19.033653447948794
9	26.895188046939367	14.492328931839943	18.848604956690195	39.763878064530495
10	15.50148238949784	26.912585344299217	32.23849722764656	25.34743503855638
11	37.06288909240577	21.28217417798383	22.226419262991723	19.42851746661868
12	23.994657538030292	24.166443173080264	29.23251559925833	22.606383689631116
13	29.281691169788726	19.516910450606805	25.6594995554336	25.54189882417087
14	23.062373600135423	20.060850119829297	25.33699206186577	31.53978421816951
15	25.001358532250666	27.344091720970837	23.005073338470517	24.649476408307983
16	25.624211606576537	25.841576573045316	24.432980323125495	24.10123149725265
17	23.544735728445108	26.275519522462528	26.001648217030095	24.178096532062277
18	24.80712568037257	24.759636588162653	27.075197596499528	23.358040134965247
19	24.889606825666103	26.158816485380697	25.471242533536714	23.480334155416482
20	24.83640915306936	24.607270605319542	26.15395580263082	24.402364438980282
21	25.86349911482821	24.931462667311724	24.594589537777278	24.61044868008279
22	24.794100610435326	25.263378336229557	25.223900548055074	24.718620505280043
23	23.48493353791431	24.919673454549343	25.778450210016267	25.81694279752008
24	23.87311130871449	26.10122797232206	25.439038863515268	24.58662185544818
25	24.454229399175663	25.231610591273018	25.265467881014793	25.048692128536526
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	169.0
1	169.0
2	449.5
3	730.0
4	730.0
5	730.0
6	1860.5
7	2991.0
8	2991.0
9	2991.0
10	3184.0
11	3377.0
12	3377.0
13	3377.0
14	3590.0
15	3803.0
16	3803.0
17	3803.0
18	6040.0
19	8277.0
20	8277.0
21	8277.0
22	13149.0
23	18021.0
24	18021.0
25	18021.0
26	26289.0
27	34557.0
28	34557.0
29	34557.0
30	42534.0
31	50511.0
32	50511.0
33	50511.0
34	62147.0
35	73783.0
36	73783.0
37	73783.0
38	83006.5
39	92230.0
40	92230.0
41	92230.0
42	105431.0
43	118632.0
44	118632.0
45	118632.0
46	140928.0
47	163224.0
48	163224.0
49	163224.0
50	157055.5
51	150887.0
52	150887.0
53	150887.0
54	137116.0
55	123345.0
56	123345.0
57	123345.0
58	112478.0
59	101611.0
60	101611.0
61	101611.0
62	88999.5
63	76388.0
64	76388.0
65	76388.0
66	62589.5
67	48791.0
68	48791.0
69	48791.0
70	36522.0
71	24253.0
72	24253.0
73	24253.0
74	18729.5
75	13206.0
76	13206.0
77	13206.0
78	11122.0
79	9038.0
80	9038.0
81	9038.0
82	6202.5
83	3367.0
84	3367.0
85	3367.0
86	2468.0
87	1569.0
88	1569.0
89	1569.0
90	1040.5
91	512.0
92	512.0
93	512.0
94	299.0
95	86.0
96	86.0
97	86.0
98	278.0
99	470.0
100	470.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008898158792982556
2	0.0032923187534035455
3	0.004627042572350929
4	0.01423705406877209
5	0.03443587452884249
6	0.044134867613193476
7	0.0671810988870183
8	0.08907056951775538
9	0.0981466914865976
10	0.11709976971565045
11	0.11959125417768555
12	0.1333834003068085
13	0.12332848087073822
14	0.12439625992589615
15	0.11505319319326446
16	0.11496421160533461
17	0.12484116786554526
18	0.13035802631719445
19	0.13133682378442252
20	0.12128190434835223
21	0.1287563577344576
22	0.1493111045462473
23	0.1364977558843524
24	0.13258256601544008
25	0.13080293425684356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1123828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.32023856837808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.42467077505594	45.04432212627737
2	11.75747345294396	13.008524727564637
3	3.1850557542985887	5.2859413254415
4	1.209897018637754	2.6772716685683977
5	0.6299933557274278	1.742569136766719
6	0.3793054924321874	1.2589962198986842
7	0.23639830759022365	0.9154327541136394
8	0.16193805908587844	0.716676164954472
9	0.13076368828464818	0.6510490588788986
>10	0.6888587218078458	7.338407530220213
>50	0.08687621088382548	3.435895866228231
>100	0.09636142294672316	11.245982226627028
>500	0.008701532162032474	3.374142125907768
>1k	0.003706208143087905	3.304789068552448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4574	0.4070017831910221	No Hit
TCCATGTACTCTGCGTTGATACCAC	4111	0.3658033079795129	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2132	0.1897087454663881	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2028	0.18045466032168622	No Hit
CATGTACTCTGCGTTGATACCACTG	1989	0.17698437839242304	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1666	0.14824332549108937	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1661	0.14779841755144027	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1364	0.12137088593628206	No Hit
CCCATGTACTCTGCGTTGATACCAC	1341	0.11932430941389609	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1339	0.11914634623803642	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1306	0.11620995383635217	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1277	0.11362948778638725	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1233	0.10971429791747493	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1189	0.10579910804856259	No Hit
GAACTACGACGGTATCTGATCGTCT	1187	0.10562114487270294	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1150	0.10232882611929939	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1126	0.1001932680089836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7796317585965112E-4
2	0.0	0.0	0.0	0.0	1.7796317585965112E-4
3	0.0	0.0	0.0	0.0	1.7796317585965112E-4
4	0.0	0.0	0.0	0.0	1.7796317585965112E-4
5	0.0	0.0	0.0	0.0	1.7796317585965112E-4
6	0.0	0.0	0.0	0.0	1.7796317585965112E-4
7	0.0	0.0	0.0	8.898158792982556E-5	1.7796317585965112E-4
8	0.0	0.0	0.0	8.898158792982556E-5	1.7796317585965112E-4
9	0.0	0.0	0.0	8.898158792982556E-5	1.7796317585965112E-4
10	8.898158792982556E-5	0.0	0.0	8.898158792982556E-5	1.7796317585965112E-4
11	8.898158792982556E-5	0.0	0.0	1.7796317585965112E-4	1.7796317585965112E-4
12	8.898158792982556E-5	0.0	0.0	1.7796317585965112E-4	2.669447637894767E-4
13	8.898158792982556E-5	0.0	0.0	1.7796317585965112E-4	2.669447637894767E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCAATT	50	8.699356E-5	15.202282	13
GTATTAC	50	8.755737E-5	15.190773	1
GCGTTAT	130	0.0	14.606513	1
CGTTATT	135	0.0	14.068036	2
CGGACAT	130	1.8189894E-12	13.882987	5
CGAACGA	55	1.9568948E-4	13.820256	16
AATACTC	55	1.9613383E-4	13.816562	5
GTAAGAC	55	1.9643056E-4	13.8141	3
GGACGCT	50	0.0014989092	13.300218	7
GACGCTC	50	0.0014989092	13.300218	8
CTTATGC	50	0.0015024362	13.296071	3
AATCGTA	60	4.0852753E-4	12.668569	13
CTAATTG	60	4.1006604E-4	12.662924	3
AAGACGG	205	0.0	12.510728	5
CCGACCA	200	0.0	12.350203	9
CTAAGAC	85	3.9535917E-6	12.290486	3
ACGGTAT	225	0.0	12.244645	9
ATTGTCG	55	0.0030671554	12.091107	6
TAGAACC	95	1.042059E-6	11.996989	4
ATCGCCA	175	0.0	11.94465	16
>>END_MODULE