FastQCFastQC Report
Thu 2 Feb 2017
SRR4062561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123828
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA34170.30405008595621397No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25750.2291275889193008No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13730.1221717202276505No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13030.1159430090725627No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC12790.1138074509622469No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA12510.11131596650021176No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11860.10553216328477312No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11660.1037525315261766No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11520.10250678929515905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC450.00139816124.444423
TATACCG500.002580341921.9999775
GTATACC550.00448344419.999983
CTACGAC3100.019.8709474
ACGGTAT3350.019.7014719
CGACGGT3250.019.630757
CGTCGTA3150.019.55553610
ATTTCGT1800.019.55553642
TACGACG3300.019.3333135
ACCGTCG3200.019.249988
CCGTCGT3250.018.9538279
GTCGTAG3400.018.76468811
ACGACGG3400.018.7646886
CGTTTTT2350.018.72338530
TGTAGGA4350.018.7126242
ATACCGT3450.018.4927356
GTATTAG3700.018.4332331
CTAGCGG4300.018.41858729
TACCGTC3350.018.388047
GAGCGCG600.007411828318.3333157
Produced by FastQC (version 0.11.5)