FastQCFastQC Report
Thu 2 Feb 2017
SRR4062560_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062560_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044371
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG102120.9778134398599733No Hit
TCCATGTACTCTGCGTTGATACCAC85530.8189618440190316No Hit
GAGTACATGGAAGCAGTGGTATCAA41360.3960278483412504No Hit
CATGTACTCTGCGTTGATACCACTG36160.34623711305656707No Hit
GCTTCCATGTACTCTGCGTTGATAC28210.27011473891940696No Hit
CCCATGTACTCTGCGTTGATACCAC24420.2338249530099936No Hit
GCGTTGATACCACTGCTTCCATGTA23800.2278883653414352No Hit
CATGGAAGCAGTGGTATCAACGCAG23740.22731385685738115No Hit
GTATCAACGCAGAGTACTTTTTTTT22710.2174514612144535No Hit
GTACATGGGAAGCAGTGGTATCAAC20350.19485412750832798No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18370.17589534753454472No Hit
GTATCAACGCAGAGTACATGGAAGC17270.16536269199355402No Hit
TATCAACGCAGAGTACTTTTTTTTT17230.164979686337518No Hit
ACGCAGAGTACATGGAAGCAGTGGT15070.14429738091157263No Hit
ACTCTGCGTTGATACCACTGCTTCC14590.1397013130391403No Hit
CAGTGGTATCAACGCAGAGTACATG13160.1260088608358524No Hit
TATCAACGCAGAGTACATGGAAGCA12990.1243810867976993No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12850.12304056700157319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCA1500.013.93611216
ATCCCGT1101.8553692E-1013.81961210
TTAGGCA551.96384E-413.8143114
CGTTATT551.9694385E-413.8096772
GTAATAC500.001504310313.2937263
TCCAACG1153.7471182E-1013.2219318
CGCATCG1450.013.10480413
GTCCTAT2000.012.8152641
CAAGACG2050.012.508694
ATCCCCG1001.437802E-712.351871512
CCGTCGT853.9352417E-612.295399
ACCGTCG853.9352417E-612.295398
TGGACCG853.9415772E-612.2936225
CGCCAGT1551.8189894E-1212.26227418
CGGTCCA1551.8189894E-1212.25933310
ACGAGAC701.0867475E-412.21847919
ACGGTAT1103.8025973E-812.092169
CGTGGAA1750.011.9440949
CGAACGA802.8583996E-511.87736916
CCCGTTT1209.973519E-911.876812
Produced by FastQC (version 0.11.5)