##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062560_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1044371
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01401321944022	32.0	32.0	32.0	32.0	32.0
2	30.63693649095963	32.0	32.0	32.0	32.0	32.0
3	30.631563879119586	32.0	32.0	32.0	32.0	32.0
4	30.691347231970248	32.0	32.0	32.0	32.0	32.0
5	30.482821717569713	32.0	32.0	32.0	32.0	32.0
6	34.105620512250916	36.0	36.0	36.0	32.0	36.0
7	33.96124270015157	36.0	36.0	36.0	32.0	36.0
8	33.9274568137185	36.0	36.0	36.0	32.0	36.0
9	34.116909603962576	36.0	36.0	36.0	32.0	36.0
10	33.75233896766571	36.0	36.0	36.0	32.0	36.0
11	34.136534813777864	36.0	36.0	36.0	32.0	36.0
12	33.8800541186992	36.0	36.0	36.0	32.0	36.0
13	33.99584438863201	36.0	36.0	36.0	32.0	36.0
14	33.88124909634603	36.0	36.0	36.0	32.0	36.0
15	33.84490951970133	36.0	36.0	36.0	32.0	36.0
16	33.8341154628001	36.0	36.0	36.0	32.0	36.0
17	33.74757054724806	36.0	36.0	36.0	32.0	36.0
18	33.783769369314165	36.0	36.0	36.0	32.0	36.0
19	33.767635256053644	36.0	36.0	36.0	32.0	36.0
20	33.74631811875282	36.0	36.0	36.0	32.0	36.0
21	33.68589514645657	36.0	36.0	36.0	27.0	36.0
22	33.643822932655155	36.0	36.0	36.0	27.0	36.0
23	33.56943844668226	36.0	36.0	36.0	27.0	36.0
24	33.56200526441274	36.0	36.0	36.0	27.0	36.0
25	33.02535593194372	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	50.0
5	85.0
6	204.0
7	52.0
8	162.0
9	199.0
10	131.0
11	32.0
12	86.0
13	62.0
14	215.0
15	277.0
16	389.0
17	556.0
18	886.0
19	1298.0
20	2009.0
21	2843.0
22	4332.0
23	6163.0
24	8402.0
25	11526.0
26	15473.0
27	19348.0
28	25239.0
29	33144.0
30	41861.0
31	55878.0
32	78698.0
33	109374.0
34	228646.0
35	396744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.120860947762644	17.20305930052064	11.393950536775668	25.282129214941047
2	15.262159015175309	20.637673221440007	37.952922836503475	26.147244926881207
3	19.18364259299335	25.08852633574702	28.482594448838576	27.245236622421054
4	11.979885406960431	16.130842535603232	36.23480560075157	35.654466456684766
5	13.37759965669972	37.81274425661696	34.474091556958726	14.335564529724593
6	33.41214339087617	35.497767899909945	17.713294886287436	13.376793822926448
7	28.852822501439686	31.220421777428996	21.643377783910214	18.283377937221108
8	27.995077687433152	33.65880400072071	20.304635177667116	18.04148313417902
9	27.00381377579139	14.8386453667752	18.56635001816312	39.59119083927029
10	15.039008948378573	27.9749476077899	32.986768210726936	23.99927523310459
11	36.542661402738446	21.428297512928133	22.250290966892088	19.778750117441334
12	23.243762032967457	24.408514930467664	30.202652430371785	22.145070606193094
13	30.158810812365484	20.252525010426005	25.330348540119747	24.258315637088774
14	23.325372727943833	19.98872540513282	25.69495509815896	30.99094676876439
15	25.227221091562356	27.609339815002105	23.939099666681685	23.22433942675385
16	25.211927976601885	25.984675424023713	25.600171018663463	23.203225580710935
17	23.238514342690596	26.32104233778187	27.028877128393923	23.41156619113361
18	24.491173436319496	24.71859695413562	28.42851499729623	22.361714612248655
19	24.347607647015394	27.38547358550446	25.47726730132919	22.78965146615096
20	24.09216022579869	24.87227398799486	27.813543511211797	23.222022274994654
21	25.054171284808646	25.88025940854545	24.38599485324855	24.679574453397354
22	23.927884730646593	26.450672484476517	25.286758888542593	24.3346838963343
23	22.917267970308945	25.964523005198142	25.51528326076138	25.602925763731538
24	23.170285026109337	27.27855007373514	25.620995625751497	23.93016927440403
25	24.619926439849813	25.564740986196927	25.161847671426212	24.653484902527047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	236.0
1	236.0
2	486.5
3	737.0
4	737.0
5	737.0
6	1908.5
7	3080.0
8	3080.0
9	3080.0
10	3208.5
11	3337.0
12	3337.0
13	3337.0
14	3493.5
15	3650.0
16	3650.0
17	3650.0
18	5865.5
19	8081.0
20	8081.0
21	8081.0
22	12857.0
23	17633.0
24	17633.0
25	17633.0
26	26450.5
27	35268.0
28	35268.0
29	35268.0
30	44690.5
31	54113.0
32	54113.0
33	54113.0
34	63185.0
35	72257.0
36	72257.0
37	72257.0
38	80015.0
39	87773.0
40	87773.0
41	87773.0
42	101222.5
43	114672.0
44	114672.0
45	114672.0
46	143368.0
47	172064.0
48	172064.0
49	172064.0
50	155462.0
51	138860.0
52	138860.0
53	138860.0
54	121870.5
55	104881.0
56	104881.0
57	104881.0
58	94025.0
59	83169.0
60	83169.0
61	83169.0
62	73007.0
63	62845.0
64	62845.0
65	62845.0
66	51399.0
67	39953.0
68	39953.0
69	39953.0
70	29980.5
71	20008.0
72	20008.0
73	20008.0
74	15282.0
75	10556.0
76	10556.0
77	10556.0
78	8494.5
79	6433.0
80	6433.0
81	6433.0
82	4440.5
83	2448.0
84	2448.0
85	2448.0
86	1858.0
87	1268.0
88	1268.0
89	1268.0
90	850.5
91	433.0
92	433.0
93	433.0
94	272.0
95	111.0
96	111.0
97	111.0
98	308.0
99	505.0
100	505.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0076601131207205105
2	0.004117310802387275
3	0.004691819286441312
4	0.014745717757386981
5	0.03705579722148546
6	0.04892897255860226
7	0.07085604636666472
8	0.09182560603463712
9	0.10034748188143869
10	0.12227455568950113
11	0.12418958396968127
12	0.13433923385463595
13	0.12505134669576234
14	0.1262003636638704
15	0.1171997307470238
16	0.11585921095089771
17	0.12744513204598748
18	0.13242420557445583
19	0.13424348244062695
20	0.1262003636638704
21	0.13242420557445583
22	0.15281925675837418
23	0.14343561818549155
24	0.13874379889905025
25	0.13548825082274404
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1044371.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.4267100215647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.61925982508255	42.53795106806301
2	12.474735449039684	13.329681468631541
3	3.6631797345428256	5.871349243028757
4	1.4322928366466745	3.0609077619794487
5	0.7208051346158559	1.9255123454588123
6	0.4144342353975534	1.3285114630556383
7	0.27375204563871186	1.0237969812104724
8	0.20625546329114788	0.8815640662095713
9	0.14020228916307803	0.6741492342727801
>10	0.8616900567417566	8.64063779283705
>50	0.09517684885624209	3.5156464672900256
>100	0.08600640393618862	8.906221908089288
>500	0.008977703711597063	3.220460476410042
>1k	0.0028728651877110603	3.2834863777237175
>5k	1.7955407423194127E-4	0.8204878750926421
>10k+	1.7955407423194127E-4	0.9796354706472655
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10212	0.9778134398599733	No Hit
TCCATGTACTCTGCGTTGATACCAC	8553	0.8189618440190316	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4136	0.3960278483412504	No Hit
CATGTACTCTGCGTTGATACCACTG	3616	0.34623711305656707	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2821	0.27011473891940696	No Hit
CCCATGTACTCTGCGTTGATACCAC	2442	0.2338249530099936	No Hit
GCGTTGATACCACTGCTTCCATGTA	2380	0.2278883653414352	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2374	0.22731385685738115	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2271	0.2174514612144535	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2035	0.19485412750832798	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1837	0.17589534753454472	No Hit
GTATCAACGCAGAGTACATGGAAGC	1727	0.16536269199355402	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1723	0.164979686337518	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1507	0.14429738091157263	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1459	0.1397013130391403	No Hit
CAGTGGTATCAACGCAGAGTACATG	1316	0.1260088608358524	No Hit
TATCAACGCAGAGTACATGGAAGCA	1299	0.1243810867976993	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1285	0.12304056700157319	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.575141400900638E-5
2	0.0	0.0	0.0	0.0	9.575141400900638E-5
3	0.0	0.0	0.0	0.0	9.575141400900638E-5
4	0.0	0.0	0.0	0.0	9.575141400900638E-5
5	0.0	9.575141400900638E-5	0.0	0.0	9.575141400900638E-5
6	0.0	9.575141400900638E-5	0.0	0.0	1.9150282801801277E-4
7	0.0	9.575141400900638E-5	0.0	0.0	1.9150282801801277E-4
8	0.0	9.575141400900638E-5	0.0	0.0	1.9150282801801277E-4
9	0.0	9.575141400900638E-5	0.0	0.0	1.9150282801801277E-4
10	0.0	9.575141400900638E-5	0.0	0.0	2.872542420270191E-4
11	0.0	9.575141400900638E-5	0.0	0.0	2.872542420270191E-4
12	0.0	1.9150282801801277E-4	0.0	0.0	4.787570700450319E-4
13	0.0	1.9150282801801277E-4	0.0	0.0	4.787570700450319E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCCA	150	0.0	13.936112	16
ATCCCGT	110	1.8553692E-10	13.819612	10
TTAGGCA	55	1.96384E-4	13.814311	4
CGTTATT	55	1.9694385E-4	13.809677	2
GTAATAC	50	0.0015043103	13.293726	3
TCCAACG	115	3.7471182E-10	13.22193	18
CGCATCG	145	0.0	13.104804	13
GTCCTAT	200	0.0	12.815264	1
CAAGACG	205	0.0	12.50869	4
ATCCCCG	100	1.437802E-7	12.3518715	12
CCGTCGT	85	3.9352417E-6	12.29539	9
ACCGTCG	85	3.9352417E-6	12.29539	8
TGGACCG	85	3.9415772E-6	12.293622	5
CGCCAGT	155	1.8189894E-12	12.262274	18
CGGTCCA	155	1.8189894E-12	12.259333	10
ACGAGAC	70	1.0867475E-4	12.218479	19
ACGGTAT	110	3.8025973E-8	12.09216	9
CGTGGAA	175	0.0	11.944094	9
CGAACGA	80	2.8583996E-5	11.877369	16
CCCGTTT	120	9.973519E-9	11.8768	12
>>END_MODULE