FastQCFastQC Report
Thu 2 Feb 2017
SRR4062560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044371
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA54350.5204089351389497No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44860.42954084324440267No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC18590.17800187864274286No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA18360.17579959612053572No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG14850.14219084980337446No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC12520.119880770339276No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA11400.10915661197026727No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10770.10312427288769986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA450.001398112924.44436544
TAGACCG551.5934513E-423.999924
TAACGGC1351.1277734E-1021.18511436
TTAACGG1401.8553692E-1020.42850535
TGGTATA3450.019.76805344
TAGGACG4300.019.4417974
GTATTAC1152.018769E-719.1322041
ATAATAC1051.7922666E-618.8570823
TATGCGT1404.0799932E-918.8570839
ATACACT4550.018.857084
CGAATGT2350.018.72334333
CGTCGTA1557.203198E-1018.45155310
GGACGTG4700.018.2552596
TTAGATA851.4306328E-418.1175884
GTCCTAA3650.018.0838641
GTATAGG1102.7932183E-618.0016651
ATCCCGT2450.017.95912620
AGGACGT4800.017.874945
GCATTCG2600.017.76917322
TTAGGAC3100.017.7418773
Produced by FastQC (version 0.11.5)