##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062560_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1044371
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23038460470465	32.0	32.0	32.0	32.0	32.0
2	31.378533107487666	32.0	32.0	32.0	32.0	32.0
3	31.47968394373264	32.0	32.0	32.0	32.0	32.0
4	31.592755831021734	32.0	32.0	32.0	32.0	32.0
5	31.529178807148035	32.0	32.0	32.0	32.0	32.0
6	35.102378369372566	36.0	36.0	36.0	36.0	36.0
7	35.11543311715856	36.0	36.0	36.0	36.0	36.0
8	35.070738272127436	36.0	36.0	36.0	36.0	36.0
9	35.186514179348144	36.0	36.0	36.0	36.0	36.0
10	35.077421720825264	36.0	36.0	36.0	36.0	36.0
11	35.20899565384332	36.0	36.0	36.0	36.0	36.0
12	35.11607082157586	36.0	36.0	36.0	36.0	36.0
13	35.14838788131804	36.0	36.0	36.0	36.0	36.0
14	35.112230232359956	36.0	36.0	36.0	36.0	36.0
15	35.07455205094741	36.0	36.0	36.0	36.0	36.0
16	35.0835632165198	36.0	36.0	36.0	36.0	36.0
17	35.060734164391775	36.0	36.0	36.0	36.0	36.0
18	35.066632451494726	36.0	36.0	36.0	36.0	36.0
19	35.03938064155363	36.0	36.0	36.0	36.0	36.0
20	35.03250185997122	36.0	36.0	36.0	36.0	36.0
21	35.00056397582851	36.0	36.0	36.0	36.0	36.0
22	34.983787370579996	36.0	36.0	36.0	36.0	36.0
23	34.912138502505336	36.0	36.0	36.0	32.0	36.0
24	34.89928579020291	36.0	36.0	36.0	32.0	36.0
25	34.86424364521803	36.0	36.0	36.0	32.0	36.0
26	34.81681796985937	36.0	36.0	36.0	32.0	36.0
27	34.78101555864726	36.0	36.0	36.0	32.0	36.0
28	34.737983915677475	36.0	36.0	36.0	32.0	36.0
29	34.726413314808624	36.0	36.0	36.0	32.0	36.0
30	34.687134169753854	36.0	36.0	36.0	32.0	36.0
31	34.675374938599404	36.0	36.0	36.0	32.0	36.0
32	34.62502693008519	36.0	36.0	36.0	32.0	36.0
33	34.59583423898212	36.0	36.0	36.0	32.0	36.0
34	34.56628631013309	36.0	36.0	36.0	32.0	36.0
35	34.49975918519377	36.0	36.0	36.0	32.0	36.0
36	34.454541537442154	36.0	36.0	36.0	32.0	36.0
37	34.40696840490592	36.0	36.0	36.0	32.0	36.0
38	34.33780237099651	36.0	36.0	36.0	32.0	36.0
39	34.227040007813315	36.0	36.0	36.0	32.0	36.0
40	34.149817449929195	36.0	36.0	36.0	32.0	36.0
41	34.15194696137675	36.0	36.0	36.0	32.0	36.0
42	34.02777844271815	36.0	36.0	36.0	32.0	36.0
43	34.05314011974672	36.0	36.0	36.0	32.0	36.0
44	34.0282859252124	36.0	36.0	36.0	32.0	36.0
45	33.86553437427887	36.0	36.0	36.0	32.0	36.0
46	33.948828529325304	36.0	36.0	36.0	32.0	36.0
47	33.872025362634545	36.0	36.0	36.0	32.0	36.0
48	33.85797001257216	36.0	36.0	36.0	32.0	36.0
49	33.8928465076108	36.0	36.0	36.0	32.0	36.0
50	33.30181803209779	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	27.0
22	97.0
23	356.0
24	859.0
25	2107.0
26	4409.0
27	8659.0
28	14227.0
29	21315.0
30	29645.0
31	41183.0
32	60599.0
33	87351.0
34	177666.0
35	595859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58395541863028	17.694739432849744	11.963861433073838	25.757443715446133
2	14.834713948796418	21.154776074098404	37.556411172212904	26.454098804892272
3	18.647425610227852	25.2506788820756	28.917599610863647	27.184295896832904
4	11.827811817470645	16.507575387626407	36.47685827518817	35.18775451971477
5	13.172426273805	37.92071974422882	34.39543993465924	14.511414047306944
6	33.63549316813007	35.61850685102023	17.422752472782634	13.323247508067066
7	28.854811991918574	31.23318364181277	21.60929555617262	18.30270881009604
8	27.973871354145224	33.766544647448086	20.204601621454447	18.054982376952253
9	27.052646999964576	14.40177867826663	18.52148326600413	40.024091055764664
10	14.91854905967324	27.941603127624187	33.16110845666913	23.97873935603344
11	36.68160069553827	21.275868441387207	22.246692028024523	19.79583883505
12	23.33710913075909	24.298357575995503	30.284257222768534	22.08027607047687
13	30.47863259320682	20.029376533817963	25.377571763291012	24.114419109684203
14	23.25409265481328	19.88708993260058	25.736639565824788	31.12217784676135
15	25.286895174224483	27.533414849703792	23.916979693997632	23.26271028207409
16	25.25453119628944	25.849626234355416	25.626908445370468	23.268934123984675
17	23.33596011379098	26.092739074524285	27.10033120414106	23.47096960754368
18	24.694768429992788	24.41536580391451	28.56551934130687	22.32434642478583
19	24.65426558186698	27.02545359838601	25.677273689139202	22.6430071306078
20	24.25450342837938	24.56272723007437	27.99072360301081	23.192045738535445
21	25.14824712673944	25.6166630440715	24.542044924648422	24.693044904540628
22	23.930001886302858	26.230908364939282	25.54379621801065	24.295293530747212
23	23.048610120349952	25.462694770345024	25.747172221365776	25.74152288793925
24	23.32083139037756	26.977769394209528	25.816687747936317	23.884711467476595
25	24.96641519153634	24.90072972152616	25.453598386014164	24.67925670092333
26	22.885353959464595	25.575968693117673	26.812693956458	24.725983390959726
27	24.81043613811567	26.102314215925183	25.958495592083658	23.128754053875493
28	23.7454889115075	24.799137471262604	26.30482845655423	25.15054516067566
29	23.237527660189723	25.93417473292537	27.013867677290925	23.814429929593985
30	24.61079443990689	25.14949189512156	26.834621030266064	23.405092634705483
31	23.427115459927556	25.302406903293946	26.111985108740093	25.158492528038405
32	23.213685558101478	26.114570396918335	26.248430873702926	24.423313171277254
33	23.092368516552067	25.534604082265783	26.528312256851255	24.844715144330895
34	23.178640540574182	26.34370353064189	27.38815995465213	23.0894959741318
35	24.905996049296657	25.64759075079641	26.393302763098553	23.053110436808375
36	23.553507326419442	26.39933510218112	26.291231755764954	23.755925815634484
37	24.79119010389986	25.86925527422726	26.150668679999733	23.188885941873146
38	23.865369681846776	25.977645874885457	25.440097436638897	24.71688700662887
39	23.91008559218898	25.574340919079525	26.349735869724455	24.16583761900704
40	25.16021605349057	25.621163360529923	25.534891336507815	23.683729249471693
41	22.98148837912964	25.727447430079923	27.113257645032274	24.177806545758166
42	25.502240104330742	26.272177224377163	25.91559895860762	22.309983712684478
43	24.123802748257084	24.757581357582698	25.98702951345834	25.131586380701876
44	23.64064111316764	25.54465798073673	27.048242434920155	23.766458471175472
45	24.326316988886134	25.738363091276952	26.209938805271303	23.72538111456561
46	23.528324704535073	25.19899537616422	26.99529190297318	24.27738801632753
47	24.746282690729636	24.757102600512653	27.06605219792583	23.43056251083188
48	23.923490790150243	27.45757972980866	24.93950904419981	23.679420435841287
49	23.113721081876076	25.63839861505155	27.53465961808591	23.713220684986467
50	23.0894959741318	27.68451058101001	24.57881346762788	24.647179977230312
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	12.0
2	16.0
3	92.5
4	169.0
5	184.0
6	199.0
7	196.5
8	194.0
9	273.5
10	353.0
11	526.5
12	700.0
13	1187.5
14	1675.0
15	2322.0
16	2969.0
17	3519.0
18	4069.0
19	4386.0
20	4703.0
21	5406.0
22	6109.0
23	6531.0
24	6953.0
25	7958.5
26	8964.0
27	11397.0
28	13830.0
29	16120.5
30	18411.0
31	19934.5
32	21458.0
33	24988.5
34	28519.0
35	32797.0
36	37075.0
37	41338.5
38	45602.0
39	47152.5
40	48703.0
41	51682.5
42	54662.0
43	57102.5
44	59543.0
45	69338.5
46	79134.0
47	86202.5
48	93271.0
49	89613.5
50	85956.0
51	78289.0
52	70622.0
53	66971.0
54	63320.0
55	62716.5
56	62113.0
57	60041.5
58	57970.0
59	52481.5
60	46993.0
61	41363.5
62	35734.0
63	30866.5
64	25999.0
65	21901.0
66	17803.0
67	15352.5
68	12902.0
69	11703.0
70	10504.0
71	8221.0
72	5938.0
73	5244.0
74	4550.0
75	3314.5
76	2079.0
77	1884.5
78	1690.0
79	1423.0
80	1156.0
81	927.0
82	698.0
83	596.0
84	494.0
85	409.0
86	324.0
87	246.5
88	169.0
89	102.0
90	35.0
91	24.5
92	14.0
93	11.0
94	8.0
95	6.0
96	4.0
97	4.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016086237553513072
2	0.002872542420270191
3	6.702598980630447E-4
4	1.9150282801801274E-4
5	0.0
6	9.575141400900637E-5
7	9.575141400900637E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1044371.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.315593117599185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50500681325083	45.56876621320236
2	12.850327200306241	14.7304825048154
3	3.536014434429574	6.080062937451692
4	1.3917824869396438	3.1908335491853173
5	0.720778308709946	2.065591813500528
6	0.4177741032889302	1.4366982311506908
7	0.2854406933186251	1.1452141846219008
8	0.1900945822131875	0.871630698239285
9	0.13786929654552957	0.7111854453791877
>10	0.7991791228262105	8.660787429943834
>50	0.08455983352519532	3.4038677758407703
>100	0.07426835737445298	8.187409459209626
>500	0.005557495608890053	2.1550080644815663
>1k	0.0011788627049160718	1.267850529739558
>5k	1.6840895784515312E-4	0.5246111632382564
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5435	0.5204089351389497	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4486	0.42954084324440267	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1859	0.17800187864274286	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1836	0.17579959612053572	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1485	0.14219084980337446	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1252	0.119880770339276	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1140	0.10915661197026727	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1077	0.10312427288769986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.575141400900638E-5
7	0.0	0.0	0.0	9.575141400900638E-5	9.575141400900638E-5
8	0.0	0.0	0.0	9.575141400900638E-5	9.575141400900638E-5
9	0.0	0.0	0.0	9.575141400900638E-5	9.575141400900638E-5
10	0.0	0.0	0.0	9.575141400900638E-5	9.575141400900638E-5
11	0.0	0.0	0.0	9.575141400900638E-5	9.575141400900638E-5
12	0.0	0.0	0.0	9.575141400900638E-5	3.8300565603602554E-4
13	0.0	0.0	0.0	9.575141400900638E-5	4.787570700450319E-4
14	0.0	0.0	0.0	9.575141400900638E-5	4.787570700450319E-4
15	0.0	0.0	0.0	9.575141400900638E-5	5.745084840540382E-4
16	0.0	0.0	0.0	9.575141400900638E-5	5.745084840540382E-4
17	0.0	0.0	0.0	9.575141400900638E-5	5.745084840540382E-4
18	0.0	0.0	0.0	2.872542420270191E-4	6.702598980630446E-4
19	0.0	0.0	0.0	2.872542420270191E-4	6.702598980630446E-4
20	0.0	9.575141400900638E-5	0.0	4.787570700450319E-4	8.617627260810574E-4
21	0.0	9.575141400900638E-5	0.0	7.660113120720511E-4	8.617627260810574E-4
22	0.0	9.575141400900638E-5	0.0	0.0015320226241441021	8.617627260810574E-4
23	0.0	9.575141400900638E-5	0.0	0.0034470509043242298	8.617627260810574E-4
24	0.0	9.575141400900638E-5	0.0	0.0061280904965764086	8.617627260810574E-4
25	0.0	9.575141400900638E-5	0.0	0.007085604636666472	8.617627260810574E-4
26	0.0	9.575141400900638E-5	0.0	0.009575141400900638	9.575141400900638E-4
27	0.0	9.575141400900638E-5	0.0	0.011394418267071759	9.575141400900638E-4
28	0.0	9.575141400900638E-5	0.0	0.016756497451576115	9.575141400900638E-4
29	0.0	9.575141400900638E-5	0.0	0.02326759360418855	9.575141400900638E-4
30	0.0	9.575141400900638E-5	0.0	0.034470509043242295	9.575141400900638E-4
31	0.0	9.575141400900638E-5	0.0	0.06329168465995322	9.575141400900638E-4
32	0.0	9.575141400900638E-5	0.0	0.1020710073336008	9.575141400900638E-4
33	0.0	9.575141400900638E-5	0.0	0.13338171971454588	9.575141400900638E-4
34	0.0	9.575141400900638E-5	0.0	0.1769486130886438	0.00105326555409907
35	0.0	9.575141400900638E-5	0.0	0.2239625573670659	0.00105326555409907
36	0.0	9.575141400900638E-5	0.0	0.29520160938976664	0.00105326555409907
37	0.0	9.575141400900638E-5	0.0	0.4071350123662951	0.00105326555409907
38	0.0	9.575141400900638E-5	0.0	0.5363994212784537	0.0011490169681080764
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	45	0.0013981129	24.444365	44
TAGACCG	55	1.5934513E-4	23.99992	4
TAACGGC	135	1.1277734E-10	21.185114	36
TTAACGG	140	1.8553692E-10	20.428505	35
TGGTATA	345	0.0	19.768053	44
TAGGACG	430	0.0	19.441797	4
GTATTAC	115	2.018769E-7	19.132204	1
ATAATAC	105	1.7922666E-6	18.857082	3
TATGCGT	140	4.0799932E-9	18.85708	39
ATACACT	455	0.0	18.85708	4
CGAATGT	235	0.0	18.723343	33
CGTCGTA	155	7.203198E-10	18.451553	10
GGACGTG	470	0.0	18.255259	6
TTAGATA	85	1.4306328E-4	18.117588	4
GTCCTAA	365	0.0	18.083864	1
GTATAGG	110	2.7932183E-6	18.001665	1
ATCCCGT	245	0.0	17.959126	20
AGGACGT	480	0.0	17.87494	5
GCATTCG	260	0.0	17.769173	22
TTAGGAC	310	0.0	17.741877	3
>>END_MODULE