FastQCFastQC Report
Thu 2 Feb 2017
SRR4062559_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062559_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251612
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23570.18831714620824985No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16420.1311908163232695No Hit
GCGCAAGACGGACCAGAGCGAAAGC16370.13079133149889902No Hit
GATTAAGAGGGACGGCCGGGGGCAT16020.12799493772830559No Hit
GAATAACGCCGCCGCATCGCCAGTC15720.12559802878208262No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15700.12543823485233443No Hit
GAATAGGACCGCGGTTCTATTTTGT14630.11688925961080592No Hit
TATCAACGCAGAGTACTTTTTTTTT14440.11537121727819805No Hit
GAACTACGACGGTATCTGATCGTCT14320.11441245369970886No Hit
GTATCTGATCGTCTTCGAACCTCCG14280.11409286584021246No Hit
GTCCTATTCCATTATTCCTAGCTGC13920.11121657510474493No Hit
GGGTAGGCACACGCTGAGCCAGTCA13630.10889956312339606No Hit
ATCAGATACCGTCGTAGTTCCGACC13360.10674234507179542No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12920.10322687861733508No Hit
TCGTAGTTCCGACCATAAACGATGC12780.1021083211090977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAC704.4288754E-714.9300815
CGTTATT1151.8189894E-1214.8633372
CCGTATG400.005274798314.251449
ACCGTAT400.005274798314.251448
TCGCGTA1301.8189894E-1213.8860199
CGAACGT1101.8735591E-1013.815714
CGCCAGT2900.013.76166318
CGCATCG3050.013.39425913
CGCGTAA1301.4551915E-1113.15517510
GCGTAAC1301.4551915E-1113.15517511
GCTCTAC655.4805125E-513.1462331
AGCCCCG2400.013.06486716
ATCGCCA3200.013.06486616
ACGGTAT3000.012.9846469
TCGCCAG3250.012.86386917
ACTCTAA2300.012.80564310
AACGTCT1352.7284841E-1112.667446
GCGTTAT1352.7284841E-1112.6593351
GCATCGC3250.012.56999814
AGGCCCG2900.012.44953310
Produced by FastQC (version 0.11.5)