##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062559_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1251612
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209414738752905	32.0	32.0	32.0	32.0	32.0
2	30.84961233992643	32.0	32.0	32.0	32.0	32.0
3	30.825298894545593	32.0	32.0	32.0	32.0	32.0
4	30.908854341441277	32.0	32.0	32.0	32.0	32.0
5	30.693286737423417	32.0	32.0	32.0	32.0	32.0
6	34.344427026906104	36.0	36.0	36.0	32.0	36.0
7	34.22922359325414	36.0	36.0	36.0	32.0	36.0
8	34.2301048567767	36.0	36.0	36.0	32.0	36.0
9	34.390580307635275	36.0	36.0	36.0	32.0	36.0
10	34.04891451983522	36.0	36.0	36.0	32.0	36.0
11	34.38106617705807	36.0	36.0	36.0	32.0	36.0
12	34.129938031914044	36.0	36.0	36.0	32.0	36.0
13	34.278340252410494	36.0	36.0	36.0	32.0	36.0
14	34.175933915622416	36.0	36.0	36.0	32.0	36.0
15	34.113825211007885	36.0	36.0	36.0	32.0	36.0
16	34.10704595353832	36.0	36.0	36.0	32.0	36.0
17	34.036905207044995	36.0	36.0	36.0	32.0	36.0
18	34.057198237153365	36.0	36.0	36.0	32.0	36.0
19	34.03350798809855	36.0	36.0	36.0	32.0	36.0
20	34.02940128410402	36.0	36.0	36.0	32.0	36.0
21	34.01151315263836	36.0	36.0	36.0	32.0	36.0
22	33.954939709750306	36.0	36.0	36.0	32.0	36.0
23	33.93492871592794	36.0	36.0	36.0	32.0	36.0
24	33.90555699370093	36.0	36.0	36.0	32.0	36.0
25	33.45192519726561	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	47.0
5	124.0
6	193.0
7	48.0
8	181.0
9	232.0
10	149.0
11	42.0
12	91.0
13	80.0
14	184.0
15	220.0
16	363.0
17	533.0
18	696.0
19	1017.0
20	1502.0
21	2244.0
22	3300.0
23	4911.0
24	7134.0
25	10374.0
26	14601.0
27	19051.0
28	25567.0
29	34464.0
30	44965.0
31	61906.0
32	89241.0
33	130674.0
34	290596.0
35	506879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06382417687787	17.639524245196707	11.037207797137	26.259443780788423
2	17.279552177032613	20.03897517190464	36.147674193770044	26.5337984572927
3	19.064904266785774	23.780173560645952	28.21523054194356	28.93969163062472
4	12.875639367029637	15.242286243050968	35.541175596296405	36.34089879362299
5	15.13555462666431	36.06358091837151	33.13445647474051	15.666407980223674
6	34.32228690551079	35.07341415591505	16.798648818235517	13.805650120338628
7	30.366625253634833	30.298189818790007	20.862574291621296	18.472610635953867
8	28.5153613638908	31.961392678362948	19.651110720168887	19.872135237577364
9	27.624739293437234	14.115820889793099	18.861719959566493	39.39771985720318
10	16.46703760822684	26.036593950175657	30.982712502159735	26.513655939437765
11	37.75837920119922	21.02605369439963	21.983219600223652	19.2323475041775
12	24.9934398320597	23.687966411940145	28.04807803079759	23.270515725202564
13	29.436806627103067	19.139231717433024	25.155442714770114	26.268518940693795
14	23.817317583966492	19.897494724346082	24.375360976319953	31.909826715367473
15	25.476800660370113	26.87496800532391	22.040172515293456	25.608058819012513
16	26.13411313776367	25.43720025467355	23.441964185732523	24.986722421830258
17	24.26831794921842	25.512539406878975	24.902347734059465	25.31679490984314
18	25.36037740528268	24.198945767590473	25.933093281728368	24.50758354539847
19	25.770221848532437	24.79901951764684	25.04686011246427	24.38389852135645
20	25.93526123641344	23.997945816921735	24.590283266087102	25.47650968057773
21	27.248865817239576	23.892036841040014	23.91195653826062	24.94714080345979
22	26.19162307183905	24.16196311949597	24.379923921758152	25.26648988690683
23	24.604515005348336	23.85054735459281	25.191832123790224	26.353105516268627
24	24.937738057427392	25.039021217461986	24.75381231894435	25.269428406166274
25	24.933101123901118	24.335591162068475	25.04013932565933	25.69116838837108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	126.0
1	126.0
2	375.0
3	624.0
4	624.0
5	624.0
6	1516.5
7	2409.0
8	2409.0
9	2409.0
10	2608.0
11	2807.0
12	2807.0
13	2807.0
14	3221.0
15	3635.0
16	3635.0
17	3635.0
18	5813.0
19	7991.0
20	7991.0
21	7991.0
22	12871.5
23	17752.0
24	17752.0
25	17752.0
26	26653.5
27	35555.0
28	35555.0
29	35555.0
30	43661.0
31	51767.0
32	51767.0
33	51767.0
34	64665.0
35	77563.0
36	77563.0
37	77563.0
38	87337.5
39	97112.0
40	97112.0
41	97112.0
42	110954.0
43	124796.0
44	124796.0
45	124796.0
46	142537.5
47	160279.0
48	160279.0
49	160279.0
50	162545.5
51	164812.0
52	164812.0
53	164812.0
54	152639.5
55	140467.0
56	140467.0
57	140467.0
58	130775.5
59	121084.0
60	121084.0
61	121084.0
62	109009.5
63	96935.0
64	96935.0
65	96935.0
66	81371.0
67	65807.0
68	65807.0
69	65807.0
70	50296.5
71	34786.0
72	34786.0
73	34786.0
74	27629.0
75	20472.0
76	20472.0
77	20472.0
78	17267.5
79	14063.0
80	14063.0
81	14063.0
82	9977.0
83	5891.0
84	5891.0
85	5891.0
86	4435.5
87	2980.0
88	2980.0
89	2980.0
90	2043.0
91	1106.0
92	1106.0
93	1106.0
94	677.5
95	249.0
96	249.0
97	249.0
98	396.5
99	544.0
100	544.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010146914539010492
2	0.0036752603842085244
3	0.00487371485732
4	0.014301556712463607
5	0.03347682828224721
6	0.04466240336462098
7	0.06375777796953049
8	0.08564954634503344
9	0.09300006711345049
10	0.11664956871618362
11	0.11688925961080592
12	0.13135061025301772
13	0.11768822925954689
14	0.12224235625737048
15	0.1121753386832341
16	0.11057739938575212
17	0.12088410785451081
18	0.12807483469317968
19	0.12903359827166885
20	0.11824750801366557
21	0.1272758650444387
22	0.1474897971575856
23	0.13510576760210033
24	0.1319098890071364
25	0.1271160711146905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1251612.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.89880175556375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.07162877948264	43.41490820562072
2	11.170223834465213	11.81782912369297
3	2.9011278546315173	4.603985617490899
4	1.170828235634909	2.477416429066701
5	0.631579030938525	1.6704886975294049
6	0.39632274442360177	1.257899897309104
7	0.27078133577222796	1.002680574008528
8	0.18551375332119127	0.7850764207894603
9	0.14174011247286028	0.674809389946183
>10	0.8290910493827202	8.300766385759049
>50	0.10067740553357465	3.7308013652846985
>100	0.1130574430305604	12.666771519159987
>500	0.013336530783702463	4.695734164698681
>1k	0.0040918901268178	2.9008322096436427
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2357	0.18831714620824985	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1642	0.1311908163232695	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1637	0.13079133149889902	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1602	0.12799493772830559	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1572	0.12559802878208262	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1570	0.12543823485233443	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1463	0.11688925961080592	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1444	0.11537121727819805	No Hit
GAACTACGACGGTATCTGATCGTCT	1432	0.11441245369970886	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1428	0.11409286584021246	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1392	0.11121657510474493	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1363	0.10889956312339606	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1336	0.10674234507179542	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1292	0.10322687861733508	No Hit
TCGTAGTTCCGACCATAAACGATGC	1278	0.1021083211090977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.989696487409837E-5
2	0.0	0.0	0.0	0.0	7.989696487409837E-5
3	0.0	0.0	0.0	0.0	7.989696487409837E-5
4	0.0	0.0	0.0	0.0	7.989696487409837E-5
5	0.0	0.0	0.0	0.0	7.989696487409837E-5
6	7.989696487409837E-5	0.0	0.0	0.0	1.5979392974819673E-4
7	7.989696487409837E-5	0.0	0.0	0.0	1.5979392974819673E-4
8	7.989696487409837E-5	0.0	0.0	0.0	1.5979392974819673E-4
9	7.989696487409837E-5	0.0	0.0	7.989696487409837E-5	1.5979392974819673E-4
10	1.5979392974819673E-4	0.0	0.0	7.989696487409837E-5	1.5979392974819673E-4
11	1.5979392974819673E-4	0.0	0.0	7.989696487409837E-5	1.5979392974819673E-4
12	1.5979392974819673E-4	0.0	0.0	7.989696487409837E-5	3.994848243704918E-4
13	1.5979392974819673E-4	0.0	0.0	7.989696487409837E-5	3.994848243704918E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGAC	70	4.4288754E-7	14.93008	15
CGTTATT	115	1.8189894E-12	14.863337	2
CCGTATG	40	0.0052747983	14.25144	9
ACCGTAT	40	0.0052747983	14.25144	8
TCGCGTA	130	1.8189894E-12	13.886019	9
CGAACGT	110	1.8735591E-10	13.81571	4
CGCCAGT	290	0.0	13.761663	18
CGCATCG	305	0.0	13.394259	13
CGCGTAA	130	1.4551915E-11	13.155175	10
GCGTAAC	130	1.4551915E-11	13.155175	11
GCTCTAC	65	5.4805125E-5	13.146233	1
AGCCCCG	240	0.0	13.064867	16
ATCGCCA	320	0.0	13.064866	16
ACGGTAT	300	0.0	12.984646	9
TCGCCAG	325	0.0	12.863869	17
ACTCTAA	230	0.0	12.805643	10
AACGTCT	135	2.7284841E-11	12.66744	6
GCGTTAT	135	2.7284841E-11	12.659335	1
GCATCGC	325	0.0	12.569998	14
AGGCCCG	290	0.0	12.449533	10
>>END_MODULE