FastQCFastQC Report
Thu 2 Feb 2017
SRR4062559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251612
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17000.1358248402859672No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16410.13111091935839542No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15480.12368050162510427No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15260.12192276839787411No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA14840.11856709587316197No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG14470.11561090817282034No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14090.11257482350760459No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13590.10857997526389969No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC12960.1035464664768315No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12780.1021083211090977No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12600.10067017574136394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCACG2000.021.99972234
TTAACGG2450.020.65279835
ATTAGCC1201.4304533E-820.1680223
TAACGGC2650.019.09409736
ATCACGG2200.018.99975835
GGTCGCA600.007412788518.33310134
GTAAACG3500.018.22833827
GTATCAA25100.017.533161
TATTAGA3400.017.472462
GTATTAG4850.017.240341
CTTATAG1307.3121555E-716.9242153
ACGATTA1307.3182855E-716.92286140
ACCGTCG4150.016.4335278
TCTAGCG4750.016.2103228
CGCAATA4650.016.08581736
TACCGTC4300.015.8602657
TCTATAC1259.4347015E-615.8410653
CCGTCGT4450.015.8200249
GTCCTAA2650.015.7765351
TATGGCG4050.015.75288516
Produced by FastQC (version 0.11.5)