##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062559_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1251612
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293574206703035	32.0	32.0	32.0	32.0	32.0
2	31.428388350383347	32.0	32.0	32.0	32.0	32.0
3	31.504184204050457	32.0	32.0	32.0	32.0	32.0
4	31.602936053665193	32.0	32.0	32.0	32.0	32.0
5	31.535383169864144	32.0	32.0	32.0	32.0	32.0
6	35.13399839566895	36.0	36.0	36.0	36.0	36.0
7	35.14486598083112	36.0	36.0	36.0	36.0	36.0
8	35.0976988076177	36.0	36.0	36.0	36.0	36.0
9	35.21416061846642	36.0	36.0	36.0	36.0	36.0
10	35.094044320444354	36.0	36.0	36.0	36.0	36.0
11	35.229023850841955	36.0	36.0	36.0	36.0	36.0
12	35.130592388056364	36.0	36.0	36.0	36.0	36.0
13	35.18195415192567	36.0	36.0	36.0	36.0	36.0
14	35.14197211276338	36.0	36.0	36.0	36.0	36.0
15	35.10602806620582	36.0	36.0	36.0	36.0	36.0
16	35.116300418979684	36.0	36.0	36.0	36.0	36.0
17	35.08154204338086	36.0	36.0	36.0	36.0	36.0
18	35.09849777726644	36.0	36.0	36.0	36.0	36.0
19	35.083318951879654	36.0	36.0	36.0	36.0	36.0
20	35.0751367037069	36.0	36.0	36.0	36.0	36.0
21	35.072294768666325	36.0	36.0	36.0	36.0	36.0
22	35.05774633033241	36.0	36.0	36.0	36.0	36.0
23	35.014890397343585	36.0	36.0	36.0	36.0	36.0
24	34.9873091660994	36.0	36.0	36.0	36.0	36.0
25	34.963376829241014	36.0	36.0	36.0	32.0	36.0
26	34.92620316839404	36.0	36.0	36.0	32.0	36.0
27	34.90581506089747	36.0	36.0	36.0	32.0	36.0
28	34.88863481654059	36.0	36.0	36.0	32.0	36.0
29	34.867203254682764	36.0	36.0	36.0	32.0	36.0
30	34.855054921173654	36.0	36.0	36.0	32.0	36.0
31	34.85272432670828	36.0	36.0	36.0	32.0	36.0
32	34.82421309479295	36.0	36.0	36.0	32.0	36.0
33	34.799837329779514	36.0	36.0	36.0	32.0	36.0
34	34.78514188103022	36.0	36.0	36.0	32.0	36.0
35	34.76648993458036	36.0	36.0	36.0	32.0	36.0
36	34.74007999284123	36.0	36.0	36.0	32.0	36.0
37	34.73380328728072	36.0	36.0	36.0	32.0	36.0
38	34.69845607105077	36.0	36.0	36.0	32.0	36.0
39	34.685890675385025	36.0	36.0	36.0	32.0	36.0
40	34.678731907332306	36.0	36.0	36.0	32.0	36.0
41	34.65599642700773	36.0	36.0	36.0	32.0	36.0
42	34.62348794993976	36.0	36.0	36.0	32.0	36.0
43	34.61866057532207	36.0	36.0	36.0	32.0	36.0
44	34.58257990495457	36.0	36.0	36.0	32.0	36.0
45	34.55047970137711	36.0	36.0	36.0	32.0	36.0
46	34.547865472686425	36.0	36.0	36.0	32.0	36.0
47	34.520882669709145	36.0	36.0	36.0	32.0	36.0
48	34.48217338919729	36.0	36.0	36.0	32.0	36.0
49	34.47588869394029	36.0	36.0	36.0	32.0	36.0
50	33.95591445272177	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	31.0
22	85.0
23	285.0
24	757.0
25	1729.0
26	3700.0
27	7014.0
28	11695.0
29	18314.0
30	26875.0
31	38832.0
32	58645.0
33	97467.0
34	219167.0
35	767009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80193225133651	18.304445456652203	11.49513740720319	26.39848488480809
2	16.67926415739566	20.737440594708094	35.87448424860895	26.708810999287298
3	18.463427729537457	24.20094534693082	28.545290682995578	28.790336240536146
4	12.563508252177796	15.807892081312936	35.82061170860868	35.807987957900586
5	14.675953889863633	36.33809838831842	33.18073013042381	15.805217591394138
6	34.40105400497121	35.416388690699236	16.559750784391586	13.622806519937969
7	30.253385234401716	30.36611985183907	20.87452021872593	18.505974695033284
8	28.429816908115296	32.1597268162977	19.50053211378606	19.90992416180094
9	27.699398855236286	13.927638916852828	18.650827892350026	39.72213433556086
10	16.443194855913816	26.108011108873995	30.96007388871311	26.488720146499073
11	37.903919105921005	20.96296615884156	22.00658031402703	19.126534421210405
12	25.002636599840844	23.65038046934673	28.045033125281638	23.30194980553079
13	29.64257293793923	19.075799848515356	25.22267284110411	26.0589543724413
14	23.905811066049225	19.76451168573008	24.415074320156727	31.914602928063967
15	25.637497882730433	26.814380175325898	21.965353480151997	25.582768461791673
16	26.234088519445326	25.449260633487054	23.368184389411415	24.948466457656206
17	24.364579438356294	25.52779934995829	24.81048439931864	25.297136812366773
18	25.535069973761836	24.133677209870154	25.977858953094092	24.353393863273922
19	25.88517847384014	24.72571371958722	25.032438167738885	24.35666963883376
20	26.06622499624484	23.906050756943845	24.55840947514086	25.469314771670454
21	27.451398676267086	23.806978520499964	23.94687810599451	24.794744697238443
22	26.315183938792536	24.131360197888803	24.363221189953435	25.190234673365232
23	24.773811692441427	23.665321201778188	25.232100682959256	26.328766422821133
24	25.143574845878753	24.79945861816601	24.85282979070191	25.20413674525332
25	25.26182235389242	24.088855012575785	24.99456700638856	25.65475562714324
26	24.83453338574574	25.027804143776187	25.41194875089085	24.72571371958722
27	25.894606315695277	24.400453175584765	24.37384748628169	25.33109302243826
28	24.778046231579754	24.22196335605603	25.427288968146676	25.57270144421754
29	24.738097749142707	24.543548639674277	25.26741514143361	25.450938469749413
30	24.602272908856737	24.772693134933192	25.640134482571277	24.984899473638794
31	25.324221883459092	24.63974458538269	24.28396340079833	25.75207013035989
32	25.04673972445135	24.549860499899328	24.37057171072185	26.03282806492747
33	24.700066793862636	24.087576661137795	25.030520640581905	26.18183590441766
34	25.48169880122594	24.230432434332684	25.395809563986283	24.892059200455094
35	26.266926172008574	24.05897354771287	25.023649501602733	24.65045077867582
36	24.689200806639757	24.752878687644415	24.858342681278224	25.699577824437604
37	26.015330629620042	24.474597558987927	24.459497032626725	25.050574778765306
38	24.907958696465037	24.17082929853661	25.227226968101935	25.693985036896414
39	25.443028670226873	24.183532915951588	24.52141718040415	25.852021233417382
40	25.744000536907603	24.28572113402556	25.257428020824346	24.71285030824249
41	24.508074387270177	24.625762616529723	25.718193817253272	25.147969178946834
42	25.891889818889556	24.938399440082073	25.08828614618588	24.081424594842492
43	24.968760286734227	23.6816201826125	25.326059513651195	26.023560017002072
44	24.46317229301093	24.452865584542174	25.173056825917296	25.910905296529595
45	24.688721424850513	24.58709248553066	25.308881666203263	25.415304423415563
46	24.42755776355433	24.314663261987953	25.51176044314088	25.74601853131684
47	24.824226677276982	23.93768995503399	25.787065000974742	25.451018366714283
48	25.46164466304254	24.975152043924158	24.74033486415918	24.822868428874123
49	24.304736611665597	24.949824706059065	25.14261608230027	25.602822599975074
50	24.13761144636792	25.306664770443852	25.021512274980008	25.53421150820822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	13.5
2	20.0
3	47.5
4	75.0
5	95.0
6	115.0
7	118.5
8	122.0
9	183.0
10	244.0
11	339.5
12	435.0
13	793.0
14	1151.0
15	1666.5
16	2182.0
17	2703.5
18	3225.0
19	3514.0
20	3803.0
21	4379.5
22	4956.0
23	5363.0
24	5770.0
25	6859.5
26	7949.0
27	10011.5
28	12074.0
29	13891.5
30	15709.0
31	16887.0
32	18065.0
33	21044.5
34	24024.0
35	28416.0
36	32808.0
37	39947.5
38	47087.0
39	50326.5
40	53566.0
41	58818.0
42	64070.0
43	64923.0
44	65776.0
45	72597.0
46	79418.0
47	84668.0
48	89918.0
49	92524.5
50	95131.0
51	91047.5
52	86964.0
53	85876.5
54	84789.0
55	86888.5
56	88988.0
57	87518.0
58	86048.0
59	79034.5
60	72021.0
61	63409.5
62	54798.0
63	48230.0
64	41662.0
65	35902.5
66	30143.0
67	26643.5
68	23144.0
69	21136.5
70	19129.0
71	15115.5
72	11102.0
73	10207.0
74	9312.0
75	6811.5
76	4311.0
77	3991.5
78	3672.0
79	3311.5
80	2951.0
81	2360.5
82	1770.0
83	1573.5
84	1377.0
85	1192.0
86	1007.0
87	758.0
88	509.0
89	324.5
90	140.0
91	92.0
92	44.0
93	28.5
94	13.0
95	12.0
96	11.0
97	8.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017737126202049838
2	0.003195878594963934
3	6.391757189927868E-4
4	2.3969089462229509E-4
5	0.0
6	3.9948482437049176E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.989696487409835E-5
47	0.0
48	0.0
49	0.0
50	7.989696487409835E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1251612.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.27805516689484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.75153099800787	46.570952266508954
2	10.887716584210697	12.254790291354508
3	2.7862232983404525	4.704096854738751
4	1.1484929057244688	2.58539788428596
5	0.5703824264911809	1.6050006832149009
6	0.3641480378540014	1.2296126017958402
7	0.24672709239693058	0.9719724641957413
8	0.18149818033121812	0.8171491684297056
9	0.13003803712681541	0.6586459044495873
>10	0.7353473578625475	7.7626069913028175
>50	0.08927240719110778	3.50917806513137
>100	0.09549089857816037	11.398027015981201
>500	0.009991568607876677	3.6900932839308993
>1k	0.0031402072767612414	2.2424765246797786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1700	0.1358248402859672	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1641	0.13111091935839542	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1548	0.12368050162510427	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1526	0.12192276839787411	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1484	0.11856709587316197	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1447	0.11561090817282034	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1409	0.11257482350760459	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1359	0.10857997526389969	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1296	0.1035464664768315	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1278	0.1021083211090977	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1260	0.10067017574136394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.989696487409837E-5
7	0.0	0.0	0.0	0.0	7.989696487409837E-5
8	0.0	0.0	0.0	1.5979392974819673E-4	7.989696487409837E-5
9	0.0	0.0	0.0	2.3969089462229509E-4	7.989696487409837E-5
10	0.0	0.0	0.0	2.3969089462229509E-4	7.989696487409837E-5
11	0.0	0.0	0.0	2.3969089462229509E-4	7.989696487409837E-5
12	0.0	0.0	0.0	2.3969089462229509E-4	2.3969089462229509E-4
13	0.0	0.0	0.0	2.3969089462229509E-4	3.994848243704918E-4
14	0.0	0.0	0.0	2.3969089462229509E-4	3.994848243704918E-4
15	0.0	0.0	0.0	2.3969089462229509E-4	4.7938178924459017E-4
16	0.0	0.0	0.0	3.1958785949639347E-4	4.7938178924459017E-4
17	0.0	0.0	0.0	3.1958785949639347E-4	4.7938178924459017E-4
18	0.0	0.0	0.0	4.7938178924459017E-4	5.592787541186886E-4
19	0.0	0.0	0.0	5.592787541186886E-4	5.592787541186886E-4
20	0.0	0.0	0.0	6.391757189927869E-4	6.391757189927869E-4
21	0.0	0.0	0.0	7.989696487409836E-4	6.391757189927869E-4
22	0.0	0.0	0.0	0.0011984544731114754	7.190726838668853E-4
23	0.0	0.0	0.0	0.0015180423326078688	7.190726838668853E-4
24	0.0	0.0	0.0	0.0024768059110970493	7.190726838668853E-4
25	0.0	0.0	0.0	0.002636599840845246	7.190726838668853E-4
26	0.0	0.0	7.989696487409837E-5	0.0035953634193344264	7.190726838668853E-4
27	0.0	0.0	7.989696487409837E-5	0.005193302716816394	7.190726838668853E-4
28	0.0	0.0	7.989696487409837E-5	0.011425265976996066	7.190726838668853E-4
29	0.0	0.0	7.989696487409837E-5	0.02876290735467541	7.190726838668853E-4
30	0.0	0.0	7.989696487409837E-5	0.0585644752527141	7.190726838668853E-4
31	0.0	0.0	7.989696487409837E-5	0.13143050721789182	7.190726838668853E-4
32	7.989696487409837E-5	0.0	7.989696487409837E-5	0.21628108391418427	7.989696487409836E-4
33	7.989696487409837E-5	0.0	7.989696487409837E-5	0.30536619974880397	7.989696487409836E-4
34	7.989696487409837E-5	0.0	7.989696487409837E-5	0.40276059993032987	8.78866613615082E-4
35	1.5979392974819673E-4	0.0	7.989696487409837E-5	0.5042297453204347	8.78866613615082E-4
36	1.5979392974819673E-4	0.0	7.989696487409837E-5	0.6599489298600525	8.78866613615082E-4
37	1.5979392974819673E-4	0.0	7.989696487409837E-5	0.8672016567434636	8.78866613615082E-4
38	1.5979392974819673E-4	0.0	7.989696487409837E-5	1.1467611368379338	8.78866613615082E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	200	0.0	21.999722	34
TTAACGG	245	0.0	20.652798	35
ATTAGCC	120	1.4304533E-8	20.168022	3
TAACGGC	265	0.0	19.094097	36
ATCACGG	220	0.0	18.999758	35
GGTCGCA	60	0.0074127885	18.333101	34
GTAAACG	350	0.0	18.228338	27
GTATCAA	2510	0.0	17.53316	1
TATTAGA	340	0.0	17.47246	2
GTATTAG	485	0.0	17.24034	1
CTTATAG	130	7.3121555E-7	16.924215	3
ACGATTA	130	7.3182855E-7	16.922861	40
ACCGTCG	415	0.0	16.433527	8
TCTAGCG	475	0.0	16.21032	28
CGCAATA	465	0.0	16.085817	36
TACCGTC	430	0.0	15.860265	7
TCTATAC	125	9.4347015E-6	15.841065	3
CCGTCGT	445	0.0	15.820024	9
GTCCTAA	265	0.0	15.776535	1
TATGGCG	405	0.0	15.752885	16
>>END_MODULE