FastQCFastQC Report
Thu 2 Feb 2017
SRR4062558_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062558_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105734
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20480.18521633593612932No Hit
GTACATGGAAGCAGTGGTATCAACG19870.1796996384302192No Hit
TCCATGTACTCTGCGTTGATACCAC17160.15519103147773333No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14660.13258161546990505No Hit
TATCAACGCAGAGTACTTTTTTTTT13590.12290478541855454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC508.7338856E-515.1951813
AAGACCG400.005279407414.2493545
CGTTATT602.5790194E-514.2429022
GCATCGC1450.013.75986914
TCGCCAG1450.013.1052317
CGGTCGG957.3659976E-813.00176710
ATCGCCA1600.012.47044516
CGCATCG1457.2759576E-1212.44940513
GTATTAG1457.2759576E-1212.440951
AACCGCG853.938392E-612.2946757
CCGCATC1551.8189894E-1212.26028612
GTCTTCG2100.012.21594611
TGGACCG1103.8113285E-812.0903625
CTAACAC550.00307477112.0870763
GATTTAG550.00308103412.0837931
GTCCATA550.00308103412.0837931
CGCCAGT1603.6379788E-1211.87715218
TCGGCGT1209.984433E-911.87607613
GAACACT2000.011.8755386
GTAAGAG1052.7281203E-711.7581773
Produced by FastQC (version 0.11.5)