##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062558_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1105734
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11722801324731	32.0	32.0	32.0	32.0	32.0
2	30.727371139894405	32.0	32.0	32.0	32.0	32.0
3	30.711352820841178	32.0	32.0	32.0	32.0	32.0
4	30.78909936747898	32.0	32.0	32.0	32.0	32.0
5	30.56007593146272	32.0	32.0	32.0	32.0	32.0
6	34.20937856663537	36.0	36.0	36.0	32.0	36.0
7	34.09152743788289	36.0	36.0	36.0	32.0	36.0
8	34.06928429441439	36.0	36.0	36.0	32.0	36.0
9	34.25107394726037	36.0	36.0	36.0	32.0	36.0
10	33.88570759332715	36.0	36.0	36.0	32.0	36.0
11	34.25104229407796	36.0	36.0	36.0	32.0	36.0
12	34.01185456900122	36.0	36.0	36.0	32.0	36.0
13	34.1306028393809	36.0	36.0	36.0	32.0	36.0
14	34.033382350547235	36.0	36.0	36.0	32.0	36.0
15	33.9640139491053	36.0	36.0	36.0	32.0	36.0
16	33.97246987069223	36.0	36.0	36.0	32.0	36.0
17	33.89544230348348	36.0	36.0	36.0	32.0	36.0
18	33.9140561834944	36.0	36.0	36.0	32.0	36.0
19	33.8983770056813	36.0	36.0	36.0	32.0	36.0
20	33.89059574906804	36.0	36.0	36.0	32.0	36.0
21	33.85632710941329	36.0	36.0	36.0	32.0	36.0
22	33.80420969238533	36.0	36.0	36.0	32.0	36.0
23	33.77704402686361	36.0	36.0	36.0	32.0	36.0
24	33.743662580693005	36.0	36.0	36.0	32.0	36.0
25	33.264607039306014	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	31.0
5	97.0
6	174.0
7	42.0
8	143.0
9	180.0
10	127.0
11	38.0
12	96.0
13	59.0
14	195.0
15	232.0
16	389.0
17	493.0
18	737.0
19	1118.0
20	1612.0
21	2436.0
22	3753.0
23	5617.0
24	7716.0
25	10697.0
26	14758.0
27	18867.0
28	25155.0
29	33295.0
30	42234.0
31	57299.0
32	80550.0
33	114248.0
34	246588.0
35	436749.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.80095366270152	17.30322566640618	10.753216260021418	26.142604410870884
2	16.490157384177653	20.052961828774844	37.45095875742289	26.005922029624617
3	19.626059186945987	23.91987576909476	28.05668532481733	28.397379719141924
4	12.55682445156377	15.196934147854826	36.02403405216001	36.2222073484214
5	14.220978600907927	36.86734082762938	34.03137761517821	14.88030295628448
6	34.151052820625566	35.3203609257113	17.019114803726517	13.509471449936623
7	29.82499640292001	30.33437912589599	21.132822291579757	18.70780217960424
8	27.824697805554678	33.53794910552254	19.78295401575801	18.85439907316477
9	27.402630671372464	14.299240452705034	18.51843470436855	39.779694171553956
10	15.400083840283354	27.226543507765182	32.55538121524952	24.817991436701945
11	37.28693863434297	21.148869980280562	22.335748315518238	19.228443069858233
12	24.236733156333422	23.69830811748641	29.532604325510807	22.532354400669362
13	29.694871917913023	19.82567472500808	25.308038935723097	25.1714144213558
14	23.00807634500118	19.888904985241656	25.55810079133694	31.544917878420225
15	24.757393621210845	27.886040412215827	22.6365625625245	24.720003404048825
16	25.16974777743577	25.913740969418242	24.7843524235456	24.132158829600392
17	23.615379182836044	26.158264626684318	25.866898938298927	24.359457252180718
18	24.197411252314616	25.12805653774238	27.235092516717295	23.4394396932257
19	25.110268184176615	25.515935574947502	25.501266352334806	23.872529888541074
20	25.047285251619105	25.053985315994048	25.8172494062747	24.081480026112143
21	25.697790341902554	24.87180578406507	24.707550578198244	24.72285329583413
22	24.90610500842723	25.52893197447107	25.153623708179712	24.411339308921995
23	23.90464331801408	25.144914083901206	25.713341332548506	25.23710126553621
24	24.29950041609354	25.59450665976045	25.73993565265109	24.36605727149492
25	24.422410865874365	25.29285795132994	25.69734012450481	24.58739105829089
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	176.0
1	176.0
2	398.5
3	621.0
4	621.0
5	621.0
6	1622.5
7	2624.0
8	2624.0
9	2624.0
10	2767.0
11	2910.0
12	2910.0
13	2910.0
14	3294.0
15	3678.0
16	3678.0
17	3678.0
18	5842.5
19	8007.0
20	8007.0
21	8007.0
22	12798.5
23	17590.0
24	17590.0
25	17590.0
26	25858.0
27	34126.0
28	34126.0
29	34126.0
30	43725.5
31	53325.0
32	53325.0
33	53325.0
34	63456.5
35	73588.0
36	73588.0
37	73588.0
38	83557.5
39	93527.0
40	93527.0
41	93527.0
42	105780.0
43	118033.0
44	118033.0
45	118033.0
46	133447.5
47	148862.0
48	148862.0
49	148862.0
50	147363.0
51	145864.0
52	145864.0
53	145864.0
54	134524.5
55	123185.0
56	123185.0
57	123185.0
58	111918.0
59	100651.0
60	100651.0
61	100651.0
62	88483.5
63	76316.0
64	76316.0
65	76316.0
66	63045.5
67	49775.0
68	49775.0
69	49775.0
70	37512.5
71	25250.0
72	25250.0
73	25250.0
74	19405.5
75	13561.0
76	13561.0
77	13561.0
78	10811.5
79	8062.0
80	8062.0
81	8062.0
82	5699.0
83	3336.0
84	3336.0
85	3336.0
86	2394.5
87	1453.0
88	1453.0
89	1453.0
90	999.5
91	546.0
92	546.0
93	546.0
94	337.5
95	129.0
96	129.0
97	129.0
98	334.0
99	539.0
100	539.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00922464173119394
2	0.0028940052490020207
3	0.00461232086559697
4	0.012299522308258586
5	0.03255755905127273
6	0.04205351377456061
7	0.06068367256501111
8	0.0813034599641505
9	0.0901663510392192
10	0.11331839303123536
11	0.11214270339882829
12	0.12679360497190104
13	0.11277576704704748
14	0.11521758397589293
15	0.10554075392454243
16	0.10436506429213536
17	0.11576020996008081
18	0.12109603213792829
19	0.12489441402724344
20	0.11458452032767374
21	0.12254303476242931
22	0.14243932084931818
23	0.13194855182168588
24	0.1279692946043081
25	0.12290478541855454
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1105734.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.66211478663293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.86678809154766	49.662124964224176
2	11.615852134666046	14.092843110753336
3	3.0167503563318547	5.490073691952562
4	1.189182322323465	2.88553258156083
5	0.6007688557667564	1.8221954644378549
6	0.3481305385994498	1.2671000815951303
7	0.23802041686482694	1.0107175294591444
8	0.17606980489583235	0.8544613372040837
9	0.11031841946497643	0.6022933762197887
>10	0.686564728614451	7.881898798829434
>50	0.07318438594161639	3.076825518911761
>100	0.07254817992913262	8.423515717124953
>500	0.0049261088917761	2.0593789511500527
>1k	8.956561621411091E-4	0.8710388765769218
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2048	0.18521633593612932	No Hit
GTACATGGAAGCAGTGGTATCAACG	1987	0.1796996384302192	No Hit
TCCATGTACTCTGCGTTGATACCAC	1716	0.15519103147773333	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1466	0.13258161546990505	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1359	0.12290478541855454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	9.043766403131313E-5
6	0.0	0.0	0.0	0.0	9.043766403131313E-5
7	0.0	0.0	0.0	0.0	9.043766403131313E-5
8	0.0	0.0	0.0	0.0	1.8087532806262626E-4
9	0.0	0.0	0.0	0.0	1.8087532806262626E-4
10	0.0	0.0	0.0	0.0	1.8087532806262626E-4
11	0.0	0.0	0.0	0.0	1.8087532806262626E-4
12	0.0	0.0	0.0	0.0	5.426259841878788E-4
13	0.0	0.0	0.0	0.0	6.33063648219192E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAC	50	8.7338856E-5	15.195181	3
AAGACCG	40	0.0052794074	14.249354	5
CGTTATT	60	2.5790194E-5	14.242902	2
GCATCGC	145	0.0	13.759869	14
TCGCCAG	145	0.0	13.10523	17
CGGTCGG	95	7.3659976E-8	13.001767	10
ATCGCCA	160	0.0	12.470445	16
CGCATCG	145	7.2759576E-12	12.449405	13
GTATTAG	145	7.2759576E-12	12.44095	1
AACCGCG	85	3.938392E-6	12.294675	7
CCGCATC	155	1.8189894E-12	12.260286	12
GTCTTCG	210	0.0	12.215946	11
TGGACCG	110	3.8113285E-8	12.090362	5
CTAACAC	55	0.003074771	12.087076	3
GATTTAG	55	0.003081034	12.083793	1
GTCCATA	55	0.003081034	12.083793	1
CGCCAGT	160	3.6379788E-12	11.877152	18
TCGGCGT	120	9.984433E-9	11.876076	13
GAACACT	200	0.0	11.875538	6
GTAAGAG	105	2.7281203E-7	11.758177	3
>>END_MODULE