FastQCFastQC Report
Thu 2 Feb 2017
SRR4062558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105734
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACG207.856471E-443.99986623
TATAACG500.002579668322.0009292
CGTACTA755.4722215E-520.533273
GTATCGA550.00448110720.0017471
TACTCCG550.00448345319.9999395
ATACCGT2050.019.3170156
TAATACT3150.018.8570864
CCGTCGT2000.018.6999449
CGTCGTA1901.8189894E-1218.52625810
TAAGGCG600.007411845518.3332775
CGCACGA600.007411845518.33327728
GATATAC2400.017.4181861
ACCGTCG2150.017.3952948
TAAGACA2300.017.2173394
CTAAGAC2055.456968E-1217.170683
TAGGACG5650.017.132694
GTCCTAC6650.016.8736551
GTACTAC1053.7710484E-516.7633691
TGTAGGA6650.016.542052
GCATAAG800.001987045216.5014421
Produced by FastQC (version 0.11.5)