##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062558_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1105734 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26083488433927 32.0 32.0 32.0 32.0 32.0 2 31.360974700967862 32.0 32.0 32.0 32.0 32.0 3 31.471915487811717 32.0 32.0 32.0 32.0 32.0 4 31.58690878638081 32.0 32.0 32.0 32.0 32.0 5 31.519474846572503 32.0 32.0 32.0 32.0 32.0 6 35.097567769463545 36.0 36.0 36.0 36.0 36.0 7 35.11617441446134 36.0 36.0 36.0 36.0 36.0 8 35.06035447946794 36.0 36.0 36.0 36.0 36.0 9 35.18656114400027 36.0 36.0 36.0 36.0 36.0 10 35.06307755753192 36.0 36.0 36.0 36.0 36.0 11 35.20479428144563 36.0 36.0 36.0 36.0 36.0 12 35.11880524610802 36.0 36.0 36.0 36.0 36.0 13 35.15915400991558 36.0 36.0 36.0 36.0 36.0 14 35.11954864370635 36.0 36.0 36.0 36.0 36.0 15 35.082693486860315 36.0 36.0 36.0 36.0 36.0 16 35.09344923824356 36.0 36.0 36.0 36.0 36.0 17 35.07492760464994 36.0 36.0 36.0 36.0 36.0 18 35.07976059341578 36.0 36.0 36.0 36.0 36.0 19 35.063427551291724 36.0 36.0 36.0 36.0 36.0 20 35.057219910032615 36.0 36.0 36.0 36.0 36.0 21 35.048712438977184 36.0 36.0 36.0 36.0 36.0 22 35.032789983847834 36.0 36.0 36.0 36.0 36.0 23 34.991769268196514 36.0 36.0 36.0 36.0 36.0 24 34.97309208182077 36.0 36.0 36.0 36.0 36.0 25 34.9449587332939 36.0 36.0 36.0 32.0 36.0 26 34.901049438653416 36.0 36.0 36.0 32.0 36.0 27 34.87806199320994 36.0 36.0 36.0 32.0 36.0 28 34.8585346927923 36.0 36.0 36.0 32.0 36.0 29 34.84086317324058 36.0 36.0 36.0 32.0 36.0 30 34.815115570290864 36.0 36.0 36.0 32.0 36.0 31 34.82367368643815 36.0 36.0 36.0 32.0 36.0 32 34.77587014598448 36.0 36.0 36.0 32.0 36.0 33 34.75958503582236 36.0 36.0 36.0 32.0 36.0 34 34.74197953576538 36.0 36.0 36.0 32.0 36.0 35 34.71176069470596 36.0 36.0 36.0 32.0 36.0 36 34.681474929775156 36.0 36.0 36.0 32.0 36.0 37 34.66291350360937 36.0 36.0 36.0 32.0 36.0 38 34.62008855656062 36.0 36.0 36.0 32.0 36.0 39 34.59473164431952 36.0 36.0 36.0 32.0 36.0 40 34.58449410075117 36.0 36.0 36.0 32.0 36.0 41 34.553130318865115 36.0 36.0 36.0 32.0 36.0 42 34.51638640034584 36.0 36.0 36.0 32.0 36.0 43 34.5067502672433 36.0 36.0 36.0 32.0 36.0 44 34.4783311356981 36.0 36.0 36.0 32.0 36.0 45 34.42884364594016 36.0 36.0 36.0 32.0 36.0 46 34.42493131259417 36.0 36.0 36.0 32.0 36.0 47 34.38892626979002 36.0 36.0 36.0 32.0 36.0 48 34.34382862424417 36.0 36.0 36.0 32.0 36.0 49 34.334630209435545 36.0 36.0 36.0 32.0 36.0 50 33.82022077642544 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 8.0 21 24.0 22 93.0 23 270.0 24 693.0 25 1771.0 26 3603.0 27 6878.0 28 11249.0 29 17644.0 30 25606.0 31 36365.0 32 54510.0 33 88553.0 34 194396.0 35 664069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.50649394112895 17.7432616146075 11.266415755145088 26.483828689118454 2 15.929300383832027 20.59524713934803 37.139999204118325 26.33545327270162 3 19.01232669819938 24.280249247103725 28.431714794750977 28.27570925994592 4 12.324494249515933 15.707046818716632 36.23596293137674 35.73249600039069 5 13.982928986537448 37.0728403033641 33.89115284507847 15.05307786501998 6 34.375539801271735 35.532150224602546 16.696827709451938 13.395482264673777 7 29.91285410931401 30.37182608443999 21.083137686166268 18.63218212007973 8 27.930406408774623 33.77494044679823 19.471048190613654 18.823604953813486 9 27.36607538521923 13.954169809375491 18.425679232075705 40.25407557332957 10 15.370514065769886 27.398090318286318 32.40924128226138 24.822154333682423 11 37.5072124037065 21.02368200670324 22.361616808382486 19.107488781207778 12 24.30521264607944 23.652795337757542 29.555209480761196 22.486782535401822 13 30.015085002360426 19.702568610533817 25.16626964532157 25.116076741784187 14 22.94032742051886 19.856583952379143 25.494648803419267 31.70843982368273 15 24.81944120376148 27.858146715213607 22.583189085259203 24.739222995765708 16 25.219808742428107 25.871321674109687 24.688939654564297 24.21992992889791 17 23.6905078436586 26.12590369835783 25.806658744327297 24.376929713656267 18 24.41292390394073 25.065069899270533 27.123069381967092 23.398936814821646 19 25.316848355933708 25.395257810648854 25.496728869691985 23.791164963725453 20 25.221165307388578 24.824505712947236 25.88181244313732 24.07251653652687 21 25.91898232305419 24.68206639209792 24.731264481330953 24.66768680351694 22 25.036943785756794 25.34262309018263 25.323812056064117 24.296621067996462 23 23.967427970922483 25.012977804788495 25.78621983225622 25.233374392032804 24 24.479757337659873 25.502788193182084 25.64359963607884 24.373854833079204 25 24.633139615856976 25.02102675688728 25.71088525811814 24.634948369137604 26 23.789808398764983 25.68294001993246 26.169585090084958 24.357666491217596 27 24.764364666366415 25.480992716150542 25.477375209589287 24.27726740789376 28 24.024765449918334 25.298037321815194 25.890946647204483 24.786250581061992 29 24.189995062103545 25.34461271879132 25.789656463489415 24.675735755615726 30 24.224723125091568 25.353566047530418 26.112247611089106 24.30946321628891 31 24.59895417885314 25.12701969913198 25.40999914988596 24.864026972128922 32 24.15807056670049 25.60642975616197 25.490850421529952 24.744649255607587 33 23.93197640662221 25.174770785740513 26.101033340749225 24.792219466888056 34 24.314256412482568 25.484700660375825 26.31971161237694 23.881331314764672 35 24.921726201780896 25.27994978900893 25.908401116362523 23.889922892847647 36 23.91886294533767 25.769760177402524 25.865624101275714 24.445752775984097 37 24.90182991569401 25.3858522935896 25.336473329028504 24.375844461687894 38 24.28468329634433 25.502155129533865 25.56311011509097 24.650051459030834 39 24.69816429629549 25.073751915017535 25.50396388281449 24.72411990587248 40 25.230751699775894 25.072847538377225 25.769488864410427 23.926911897436455 41 24.008124919736574 25.309342029819103 26.399658507380618 24.282874543063706 42 25.13307902262208 25.86517191295556 25.61818665248604 23.383562411936325 43 24.160331508301272 24.969205975397337 26.065220025792822 24.805242490508565 44 23.812689127764905 25.551714969423028 25.92721215048104 24.70838375233103 45 24.053615064744324 25.67733288476252 25.747331636722752 24.5217204137704 46 23.97965874220992 25.017974501981943 25.97408235080259 25.028284405005547 47 24.604651751687115 24.994618958990138 26.013941870287066 24.386787419035684 48 24.56910974972281 25.9377933571727 25.219718304764076 24.273378588340414 49 24.06021701421861 25.531728245672102 25.73394686244612 24.67410787766316 50 23.745765256381734 26.347566412898583 25.113544487191312 24.793123843528374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 19.0 1 23.0 2 27.0 3 103.0 4 179.0 5 204.5 6 230.0 7 203.5 8 177.0 9 240.0 10 303.0 11 458.0 12 613.0 13 1030.5 14 1448.0 15 1990.5 16 2533.0 17 2886.0 18 3239.0 19 3572.0 20 3905.0 21 4554.0 22 5203.0 23 5719.5 24 6236.0 25 7422.0 26 8608.0 27 10821.0 28 13034.0 29 15329.0 30 17624.0 31 19568.0 32 21512.0 33 25313.0 34 29114.0 35 33489.5 36 37865.0 37 42527.5 38 47190.0 39 49308.5 40 51427.0 41 54671.5 42 57916.0 43 59301.5 44 60687.0 45 66578.5 46 72470.0 47 77038.5 48 81607.0 49 82566.5 50 83526.0 51 80323.0 52 77120.0 53 75205.5 54 73291.0 55 72976.0 56 72661.0 57 70609.5 58 68558.0 59 62560.5 60 56563.0 61 49863.0 62 43163.0 63 37630.5 64 32098.0 65 27365.0 66 22632.0 67 19750.0 68 16868.0 69 15131.0 70 13394.0 71 10549.0 72 7704.0 73 6799.5 74 5895.0 75 4374.0 76 2853.0 77 2544.5 78 2236.0 79 1918.0 80 1600.0 81 1264.5 82 929.0 83 797.5 84 666.0 85 565.5 86 465.0 87 349.5 88 234.0 89 155.0 90 76.0 91 45.0 92 14.0 93 14.0 94 14.0 95 8.0 96 2.0 97 3.5 98 5.0 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014922214565166668 2 0.0037983818893151514 3 3.617506561252526E-4 4 9.043766403131315E-5 5 0.0 6 2.7131299209393945E-4 7 1.808753280626263E-4 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 9.043766403131315E-5 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1105734.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.572520946053466 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02163321813875 52.143219957896406 2 11.730070359173958 14.914202872145346 3 3.016235107587038 5.752490086658964 4 1.1032441780414521 2.8054405446860704 5 0.5728949313792274 1.8210187512496898 6 0.3201647159985151 1.2212206868401716 7 0.2270254876970257 1.0102807800335019 8 0.15192193145943583 0.7726448135895907 9 0.11220028963343405 0.6419569736587276 >10 0.6176220859985543 7.321258097991572 >50 0.06401608454734703 2.8290302293011775 >100 0.05939368032565105 7.1816717626149575 >500 0.003577930019691784 1.5855644433338711 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.713129920939394E-4 2 0.0 0.0 0.0 0.0 2.713129920939394E-4 3 0.0 0.0 0.0 0.0 2.713129920939394E-4 4 0.0 0.0 0.0 0.0 2.713129920939394E-4 5 0.0 0.0 0.0 0.0 2.713129920939394E-4 6 0.0 0.0 0.0 0.0 2.713129920939394E-4 7 0.0 0.0 0.0 0.0 2.713129920939394E-4 8 0.0 0.0 0.0 0.0 2.713129920939394E-4 9 0.0 0.0 0.0 9.043766403131313E-5 2.713129920939394E-4 10 0.0 0.0 0.0 9.043766403131313E-5 3.6175065612525253E-4 11 0.0 0.0 0.0 9.043766403131313E-5 3.6175065612525253E-4 12 0.0 0.0 0.0 9.043766403131313E-5 3.6175065612525253E-4 13 0.0 0.0 0.0 9.043766403131313E-5 3.6175065612525253E-4 14 0.0 0.0 0.0 9.043766403131313E-5 3.6175065612525253E-4 15 0.0 0.0 0.0 9.043766403131313E-5 3.6175065612525253E-4 16 0.0 0.0 0.0 9.043766403131313E-5 3.6175065612525253E-4 17 0.0 0.0 0.0 9.043766403131313E-5 4.5218832015656566E-4 18 0.0 0.0 0.0 3.6175065612525253E-4 4.5218832015656566E-4 19 0.0 0.0 0.0 3.6175065612525253E-4 4.5218832015656566E-4 20 0.0 0.0 0.0 4.5218832015656566E-4 4.5218832015656566E-4 21 0.0 0.0 0.0 8.139389762818182E-4 5.426259841878788E-4 22 0.0 0.0 0.0 0.0014470026245010101 5.426259841878788E-4 23 0.0 0.0 0.0 0.001899190944657576 5.426259841878788E-4 24 0.0 0.0 0.0 0.0045218832015656565 5.426259841878788E-4 25 0.0 0.0 0.0 0.005607135169941414 5.426259841878788E-4 26 0.0 0.0 0.0 0.006873262466379798 6.33063648219192E-4 27 0.0 0.0 0.0 0.009043766403131313 6.33063648219192E-4 28 0.0 0.0 0.0 0.015374402885323234 6.33063648219192E-4 29 0.0 0.0 0.0 0.021252851047358585 6.33063648219192E-4 30 0.0 0.0 0.0 0.03490893831608687 6.33063648219192E-4 31 0.0 0.0 0.0 0.06656212072704647 6.33063648219192E-4 32 9.043766403131313E-5 0.0 0.0 0.10481725261229193 6.33063648219192E-4 33 9.043766403131313E-5 0.0 0.0 0.1472325170429778 6.33063648219192E-4 34 9.043766403131313E-5 0.0 0.0 0.1900999697938202 9.043766403131313E-4 35 9.043766403131313E-5 0.0 0.0 0.2408354993153869 9.043766403131313E-4 36 9.043766403131313E-5 0.0 0.0 0.3132760682044687 9.043766403131313E-4 37 9.043766403131313E-5 0.0 0.0 0.42333870533057677 9.043766403131313E-4 38 9.043766403131313E-5 0.0 0.0 0.5618892066265485 9.043766403131313E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGACG 20 7.856471E-4 43.999866 23 TATAACG 50 0.0025796683 22.000929 2 CGTACTA 75 5.4722215E-5 20.53327 3 GTATCGA 55 0.004481107 20.001747 1 TACTCCG 55 0.004483453 19.999939 5 ATACCGT 205 0.0 19.317015 6 TAATACT 315 0.0 18.857086 4 CCGTCGT 200 0.0 18.699944 9 CGTCGTA 190 1.8189894E-12 18.526258 10 TAAGGCG 60 0.0074118455 18.333277 5 CGCACGA 60 0.0074118455 18.333277 28 GATATAC 240 0.0 17.418186 1 ACCGTCG 215 0.0 17.395294 8 TAAGACA 230 0.0 17.217339 4 CTAAGAC 205 5.456968E-12 17.17068 3 TAGGACG 565 0.0 17.13269 4 GTCCTAC 665 0.0 16.873655 1 GTACTAC 105 3.7710484E-5 16.763369 1 TGTAGGA 665 0.0 16.54205 2 GCATAAG 80 0.0019870452 16.501442 1 >>END_MODULE