FastQCFastQC Report
Thu 2 Feb 2017
SRR4062557_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062557_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946534
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG98091.0363072007978582No Hit
TCCATGTACTCTGCGTTGATACCAC83250.879524665780627No Hit
GAGTACATGGAAGCAGTGGTATCAA42940.45365512490834986No Hit
CATGTACTCTGCGTTGATACCACTG38490.4066414941248809No Hit
GTATCAACGCAGAGTACTTTTTTTT28970.30606401883080797No Hit
GCTTCCATGTACTCTGCGTTGATAC26290.27775019175222443No Hit
CCCATGTACTCTGCGTTGATACCAC23910.2526058229287062No Hit
GTACATGGGAAGCAGTGGTATCAAC23230.24542171755055814No Hit
GCGTTGATACCACTGCTTCCATGTA23080.24383698842302548No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22970.2426748537295015No Hit
CATGGAAGCAGTGGTATCAACGCAG22050.2329551817473012No Hit
TATCAACGCAGAGTACTTTTTTTTT20230.21372713499990492No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18050.19069573834643022No Hit
GTATCAACGCAGAGTACATGGAAGC17450.1843568218362996No Hit
ACTCTGCGTTGATACCACTGCTTCC15370.16238191126784668No Hit
CAGTGGTATCAACGCAGAGTACATG14390.15202834763463333No Hit
ACGCAGAGTACATGGAAGCAGTGGT13930.14716851164353315No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13580.14347081034595693No Hit
GTACATGGGGTGGTATCAACGCAAA11200.11832644152243871No Hit
TATCAACGCAGAGTACATGGAAGCA11020.11642476656939951No Hit
GTATCAACGCAGAGTACATGGGAAG10740.11346660553133855No Hit
GCGCAAGACGGACCAGAGCGAAAGC10240.10818417510622969No Hit
GGTATCAACGCAGAGTACTTTTTTT10210.10786722928072315No Hit
GAATAGGACCGCGGTTCTATTTTGT9820.10374693354913822No Hit
GAATAACGCCGCCGCATCGCCAGTC9780.1033243391151295No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9770.10321869050662733No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9520.10057747529407289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTG250.006030659718.9998427
AACCGCG855.638867E-1116.7645657
GAGGCGT350.002168708716.286446
CGCGTAA807.701601E-915.43737110
TCGCGTA807.701601E-915.4373719
GCGTAAC953.0377123E-1014.99987511
CGCGGCG704.4177978E-714.93239616
TAGAACC1151.8189894E-1214.8670824
CGCATCG1350.014.77843613
GTCGCGT851.7138518E-814.529298
ACACCGT400.0052755214.2506356
CGTCGTA1450.013.75850610
CGAGCCG1550.013.48518515
TCCGAGT500.001497092913.30212
CCGTCGT1500.013.2998899
TTAGAAC500.001502472313.29567053
GCTAGCA500.00150307113.2949682
ACGGTAT1750.013.0284639
CGCGGTC1102.741217E-912.95443710
CGACCCG1102.744855E-912.9530675
Produced by FastQC (version 0.11.5)