##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062557_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	946534
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.109942167951704	32.0	32.0	32.0	32.0	32.0
2	30.829859254923754	32.0	32.0	32.0	32.0	32.0
3	30.826896867941354	32.0	32.0	32.0	32.0	32.0
4	30.874241178869433	32.0	32.0	32.0	32.0	32.0
5	30.71311648604276	32.0	32.0	32.0	32.0	32.0
6	34.364742312479	36.0	36.0	36.0	32.0	36.0
7	34.20881341821847	36.0	36.0	36.0	32.0	36.0
8	34.18916805946749	36.0	36.0	36.0	32.0	36.0
9	34.32047765848876	36.0	36.0	36.0	32.0	36.0
10	34.044266766962416	36.0	36.0	36.0	32.0	36.0
11	34.3540633511316	36.0	36.0	36.0	32.0	36.0
12	34.15136593085933	36.0	36.0	36.0	32.0	36.0
13	34.24559815072676	36.0	36.0	36.0	32.0	36.0
14	34.156842754724074	36.0	36.0	36.0	32.0	36.0
15	34.11484637635838	36.0	36.0	36.0	32.0	36.0
16	34.10041055049264	36.0	36.0	36.0	32.0	36.0
17	34.0226130281638	36.0	36.0	36.0	32.0	36.0
18	34.029755930584635	36.0	36.0	36.0	32.0	36.0
19	34.027484485501844	36.0	36.0	36.0	32.0	36.0
20	34.00126883978811	36.0	36.0	36.0	32.0	36.0
21	33.94957814510625	36.0	36.0	36.0	32.0	36.0
22	33.898436823188604	36.0	36.0	36.0	32.0	36.0
23	33.82308084020225	36.0	36.0	36.0	32.0	36.0
24	33.808087189683626	36.0	36.0	36.0	32.0	36.0
25	33.29414791227785	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	35.0
5	100.0
6	153.0
7	34.0
8	127.0
9	184.0
10	123.0
11	38.0
12	54.0
13	60.0
14	210.0
15	270.0
16	384.0
17	476.0
18	675.0
19	1001.0
20	1456.0
21	2105.0
22	3115.0
23	4388.0
24	6043.0
25	8115.0
26	11160.0
27	14417.0
28	19331.0
29	25874.0
30	33576.0
31	45973.0
32	66936.0
33	96532.0
34	213001.0
35	390583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.34971752665984	16.853837611903714	11.435970420334277	25.36047444110217
2	15.823293681860587	20.632183725886083	36.85907086868738	26.68545172356595
3	19.532148049225775	24.88800797890324	27.951412367300023	27.628431604570963
4	12.6176978713737	15.54293442943411	35.143836810600334	36.695530888591854
5	14.155540689213483	36.86151790989094	33.70692944159271	15.27601195930286
6	34.26294399611033	34.196671581606495	17.432181757645903	14.108202664637274
7	29.522645604561156	30.67593754526108	21.301277829286576	18.500139020891194
8	28.59579480758982	32.25588143235401	20.447335696339596	18.700988063716576
9	27.31334507868895	14.863127126906948	18.480217253828695	39.34331054057541
10	15.738167894522512	27.311921219781787	32.043705674227716	24.90620521146798
11	37.14430475078326	21.421046285072382	21.224410356652516	20.21023860749184
12	23.867561354641943	24.150439603801193	29.26552154222598	22.71647749933089
13	30.441115338603066	19.670847970010545	24.9111750699969	24.97686162138949
14	24.090949960596813	19.52652707184074	24.985851875750377	31.396671091812074
15	25.523086458666068	26.38141152429647	24.059332843969937	24.036169173067528
16	25.892902472457735	25.503143456702375	24.840923321656426	23.763030749183464
17	23.93014754105297	25.611298928243514	26.49286412429498	23.965689406408536
18	25.367630335413427	23.933922275314686	27.750867174929734	22.94758021434216
19	24.598427562510313	26.981415109110067	25.40453451026358	23.01562281811604
20	24.57573154839474	23.799757137779885	27.555342122632727	24.06916919119265
21	25.732810465260197	25.322781469143624	24.08507784309515	24.85933022250103
22	24.373422777604368	25.59890804628106	25.209952438643736	24.817716737470835
23	23.281291492497363	25.048428852699058	25.3504810088013	26.31979864600228
24	23.418872499494846	26.847668205222057	25.294443110300985	24.439016184982105
25	25.012456349882207	24.871548850577753	24.722707370456607	25.393287429083433
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	207.0
2	499.0
3	791.0
4	791.0
5	791.0
6	2065.0
7	3339.0
8	3339.0
9	3339.0
10	3424.0
11	3509.0
12	3509.0
13	3509.0
14	3263.5
15	3018.0
16	3018.0
17	3018.0
18	4708.5
19	6399.0
20	6399.0
21	6399.0
22	10080.0
23	13761.0
24	13761.0
25	13761.0
26	20473.5
27	27186.0
28	27186.0
29	27186.0
30	33546.5
31	39907.0
32	39907.0
33	39907.0
34	48113.0
35	56319.0
36	56319.0
37	56319.0
38	63779.0
39	71239.0
40	71239.0
41	71239.0
42	85752.5
43	100266.0
44	100266.0
45	100266.0
46	129421.5
47	158577.0
48	158577.0
49	158577.0
50	144402.0
51	130227.0
52	130227.0
53	130227.0
54	115212.0
55	100197.0
56	100197.0
57	100197.0
58	91266.0
59	82335.0
60	82335.0
61	82335.0
62	72866.5
63	63398.0
64	63398.0
65	63398.0
66	52110.5
67	40823.0
68	40823.0
69	40823.0
70	30808.5
71	20794.0
72	20794.0
73	20794.0
74	16192.5
75	11591.0
76	11591.0
77	11591.0
78	9454.0
79	7317.0
80	7317.0
81	7317.0
82	5091.5
83	2866.0
84	2866.0
85	2866.0
86	2143.5
87	1421.0
88	1421.0
89	1421.0
90	957.0
91	493.0
92	493.0
93	493.0
94	304.0
95	115.0
96	115.0
97	115.0
98	277.0
99	439.0
100	439.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007712348420658951
2	0.003908998514580564
3	0.0040146471230827424
4	0.015741642666824433
5	0.03433579776320766
6	0.04637973913245588
7	0.06687556918187831
8	0.08937872279284209
9	0.0963515309539858
10	0.11822079291393653
11	0.11959422482446483
12	0.13216640923622397
13	0.11991117064997137
14	0.12455970942406716
15	0.11494568605036903
16	0.11388919996534726
17	0.12360887194754758
18	0.12920824819816298
19	0.1320607606277218
20	0.1206507109094866
21	0.1304760315001891
22	0.15202834763463333
23	0.14082959513340249
24	0.1347019758402762
25	0.13026473428318477
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	946534.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.08440493603619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30687351294172	42.630425927080374
2	12.367516220437794	13.130444781974312
3	3.3833387699816675	5.3880757590449235
4	1.3576539418213704	2.8828100644260535
5	0.6688504032359006	1.775276282350282
6	0.38333662940439595	1.2209518117270914
7	0.2597016005604788	0.9650273448682425
8	0.18506859171935955	0.7859404851017947
9	0.137456018409791	0.6567093847944531
>10	0.757292723837787	7.635935538979215
>50	0.08817834672805128	3.3690541131182803
>100	0.09096830070895914	9.84596708300183
>500	0.00917662680853339	3.362825323030171
>1k	0.004189329629982634	4.430182162293943
>5k	3.989837742840604E-4	1.9203739382090732
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	9809	1.0363072007978582	No Hit
TCCATGTACTCTGCGTTGATACCAC	8325	0.879524665780627	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4294	0.45365512490834986	No Hit
CATGTACTCTGCGTTGATACCACTG	3849	0.4066414941248809	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2897	0.30606401883080797	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2629	0.27775019175222443	No Hit
CCCATGTACTCTGCGTTGATACCAC	2391	0.2526058229287062	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2323	0.24542171755055814	No Hit
GCGTTGATACCACTGCTTCCATGTA	2308	0.24383698842302548	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2297	0.2426748537295015	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2205	0.2329551817473012	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2023	0.21372713499990492	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1805	0.19069573834643022	No Hit
GTATCAACGCAGAGTACATGGAAGC	1745	0.1843568218362996	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1537	0.16238191126784668	No Hit
CAGTGGTATCAACGCAGAGTACATG	1439	0.15202834763463333	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1393	0.14716851164353315	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1358	0.14347081034595693	No Hit
GTACATGGGGTGGTATCAACGCAAA	1120	0.11832644152243871	No Hit
TATCAACGCAGAGTACATGGAAGCA	1102	0.11642476656939951	No Hit
GTATCAACGCAGAGTACATGGGAAG	1074	0.11346660553133855	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1024	0.10818417510622969	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1021	0.10786722928072315	No Hit
GAATAGGACCGCGGTTCTATTTTGT	982	0.10374693354913822	No Hit
GAATAACGCCGCCGCATCGCCAGTC	978	0.1033243391151295	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	977	0.10321869050662733	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	952	0.10057747529407289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0564860850217741E-4	0.0
5	0.0	0.0	0.0	1.0564860850217741E-4	0.0
6	0.0	0.0	0.0	1.0564860850217741E-4	0.0
7	0.0	0.0	0.0	1.0564860850217741E-4	0.0
8	0.0	0.0	0.0	1.0564860850217741E-4	0.0
9	0.0	0.0	0.0	1.0564860850217741E-4	0.0
10	0.0	0.0	0.0	1.0564860850217741E-4	0.0
11	0.0	0.0	0.0	2.1129721700435483E-4	0.0
12	0.0	0.0	0.0	3.169458255065322E-4	0.0
13	0.0	0.0	0.0	4.2259443400870965E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGTG	25	0.0060306597	18.999842	7
AACCGCG	85	5.638867E-11	16.764565	7
GAGGCGT	35	0.0021687087	16.28644	6
CGCGTAA	80	7.701601E-9	15.437371	10
TCGCGTA	80	7.701601E-9	15.437371	9
GCGTAAC	95	3.0377123E-10	14.999875	11
CGCGGCG	70	4.4177978E-7	14.932396	16
TAGAACC	115	1.8189894E-12	14.867082	4
CGCATCG	135	0.0	14.778436	13
GTCGCGT	85	1.7138518E-8	14.52929	8
ACACCGT	40	0.00527552	14.250635	6
CGTCGTA	145	0.0	13.758506	10
CGAGCCG	155	0.0	13.485185	15
TCCGAGT	50	0.0014970929	13.302	12
CCGTCGT	150	0.0	13.299889	9
TTAGAAC	50	0.0015024723	13.2956705	3
GCTAGCA	50	0.001503071	13.294968	2
ACGGTAT	175	0.0	13.028463	9
CGCGGTC	110	2.741217E-9	12.954437	10
CGACCCG	110	2.744855E-9	12.953067	5
>>END_MODULE