FastQCFastQC Report
Thu 2 Feb 2017
SRR4062557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946534
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40480.4276655672168142No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36100.38139147669286044No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15820.16713609865044468No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC14650.15477521145568993No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11010.11631911796089733No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9970.10533166267667089No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9830.10385258215764039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTA250.002352632835.19970342
ATTACAC1001.7425918E-924.2010753
CCTAATA551.5922113E-424.0023332
TAGGACG1750.021.3723774
CGCAATA3100.020.58047336
TGGTATA3100.020.58047344
ATATTAT755.4647106E-520.5364131
AACGGAC755.4713833E-520.5331615
TCGTATA550.00448312419.9998344
TATACTG1051.7919938E-618.8569855
AGGACGT2000.018.6998425
ATACGAA3450.018.49259840
ACGCCTT600.00741130418.3331811
CTAGCGG3650.018.0820429
TACGAAT3550.017.97168241
CGAATGC3800.017.94721843
CGACGGT3200.017.874857
GTATTAC1002.478891E-517.6026421
GTCTAAC1002.478891E-517.6026421
TCTAGCG3750.017.59985228
Produced by FastQC (version 0.11.5)