FastQCFastQC Report
Thu 2 Feb 2017
SRR4062556_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062556_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314019
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG58740.44702549963128385No Hit
TCCATGTACTCTGCGTTGATACCAC52460.3992331922141156No Hit
GTATCAACGCAGAGTACTTTTTTTT34580.26316210039580856No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28100.21384774497172415No Hit
TATCAACGCAGAGTACTTTTTTTTT26900.20471545693022705No Hit
GAGTACATGGAAGCAGTGGTATCAA25810.19642029529253385No Hit
CATGTACTCTGCGTTGATACCACTG21300.16209811273657382No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19070.14512727745945836No Hit
CCCATGTACTCTGCGTTGATACCAC15890.120926714149491No Hit
GCTTCCATGTACTCTGCGTTGATAC14990.11407749811836816No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14650.11149001650661064No Hit
GTACATGGGAAGCAGTGGTATCAAC14380.10943525169727379No Hit
GAATAGGACCGCGGTTCTATTTTGT14350.10920694449623636No Hit
GATTAAGAGGGACGGCCGGGGGCAT14290.1087503300941615No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14210.10814151089139502No Hit
GAATAACGCCGCCGCATCGCCAGTC14000.10654336048413302No Hit
GCGTTGATACCACTGCTTCCATGTA13500.10273824046684256No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13350.10159670446165542No Hit
GCGCAAGACGGACCAGAGCGAAAGC13300.10121619245992639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTG400.005276626414.2507727
GTCTTAC801.2942473E-714.2442581
GCGTAAC957.374365E-813.00119911
CCGCATC2050.012.97730312
GCGTTAT1102.761226E-912.9493251
GCTCGTA1251.0186341E-1012.9216859
AATCCCG751.4756091E-512.66880119
CGAACGA751.477345E-512.66735316
CGACGGG1650.012.6668714
CGCATCG2050.012.51239613
CGAACCT3050.012.45969116
CGTTATT1155.349648E-912.3867842
CGCGTAA1001.4392936E-712.35160910
TCGCGTA1001.4392936E-712.3516099
TAGAGTG1850.012.3226435
GTTATTC1405.2750693E-1112.2107593
TCTTATA701.09437315E-412.2098282
CCGTCAA1950.012.18200318
CGTCAAT1950.012.18153919
TCCGTCA2050.012.05081217
Produced by FastQC (version 0.11.5)