##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062556_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1314019
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05674727686586	32.0	32.0	32.0	32.0	32.0
2	30.689919247742992	32.0	32.0	32.0	32.0	32.0
3	30.67530530380459	32.0	32.0	32.0	32.0	32.0
4	30.74498085644119	32.0	32.0	32.0	32.0	32.0
5	30.514307631777015	32.0	32.0	32.0	32.0	32.0
6	34.14891032778065	36.0	36.0	36.0	32.0	36.0
7	34.010415374511325	36.0	36.0	36.0	32.0	36.0
8	34.001740461895906	36.0	36.0	36.0	32.0	36.0
9	34.182481379645196	36.0	36.0	36.0	32.0	36.0
10	33.81254152337219	36.0	36.0	36.0	32.0	36.0
11	34.1895254178212	36.0	36.0	36.0	32.0	36.0
12	33.93432819464559	36.0	36.0	36.0	32.0	36.0
13	34.06572583805866	36.0	36.0	36.0	32.0	36.0
14	33.966140520038145	36.0	36.0	36.0	32.0	36.0
15	33.903333209032745	36.0	36.0	36.0	32.0	36.0
16	33.89442542307227	36.0	36.0	36.0	32.0	36.0
17	33.815014090359426	36.0	36.0	36.0	32.0	36.0
18	33.82951920786534	36.0	36.0	36.0	32.0	36.0
19	33.81772637990775	36.0	36.0	36.0	32.0	36.0
20	33.80086284901512	36.0	36.0	36.0	32.0	36.0
21	33.759091002489306	36.0	36.0	36.0	32.0	36.0
22	33.72133431860574	36.0	36.0	36.0	27.0	36.0
23	33.67016610870923	36.0	36.0	36.0	27.0	36.0
24	33.64963139802393	36.0	36.0	36.0	27.0	36.0
25	33.16894124057567	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	64.0
5	113.0
6	216.0
7	61.0
8	213.0
9	232.0
10	153.0
11	47.0
12	104.0
13	75.0
14	311.0
15	358.0
16	506.0
17	693.0
18	1015.0
19	1457.0
20	2329.0
21	3288.0
22	4858.0
23	7093.0
24	9688.0
25	13596.0
26	18304.0
27	23158.0
28	30867.0
29	40495.0
30	51185.0
31	69367.0
32	97707.0
33	139037.0
34	294294.0
35	503123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.320579464830715	17.4966626125845	11.435936622366052	25.746821300218738
2	16.300588064728977	20.390480459614405	37.07874831331894	26.230183162337678
3	18.94674113982287	24.41958961627653	28.42357457450525	28.210094669395353
4	12.55012102114445	15.531503554520407	35.61743617847194	36.300939245863205
5	14.40278935568472	36.72136513990553	33.50500355901687	15.370841945392883
6	33.87642139004047	35.078174072932015	17.18702771020584	13.858376826821683
7	29.820809820809817	30.767631536862304	21.038967962044886	18.37259068028299
8	28.297292150664582	32.576912823247135	19.895951555775603	19.22984347031268
9	27.47896221463683	14.52949784308901	18.880722022430653	39.110817919843505
10	15.997900176077398	26.638506473568203	31.47633012340656	25.887263226947844
11	36.893439119333834	21.316366733944854	22.1782590111249	19.611935135596408
12	24.539715077136446	24.03517528585635	28.745584991065204	22.679524645942
13	29.61195822120088	19.51272290610742	25.33959751180248	25.53572136088922
14	23.68697162842263	19.91991801311333	25.118618109501263	31.274492248962783
15	25.336679557859515	26.930250418278067	23.026093237152235	24.706976786710186
16	25.685685029819684	25.64606767254121	24.240565735861935	24.427681561777174
17	23.772166828960543	25.832841880048825	25.94172354571735	24.453267745273287
18	25.02800405392019	24.387835191380088	26.980743879113927	23.6034168755858
19	25.262210585352175	25.77558248698472	25.428711639985856	23.533495287677244
20	25.20675199361191	24.142324003651208	26.17586148568851	24.475062517048375
21	26.486603877103143	24.572817605462085	24.252697512801756	24.687881004633017
22	25.25627443657732	24.968027620477564	24.775659835222203	25.00003810772291
23	23.764101071235604	24.627673802394867	25.52233880979071	26.085886316578815
24	24.108818654626507	25.872701987007297	25.331504448381626	24.686974909984567
25	24.84369201554812	24.725960955960964	25.182559343693157	25.247787684797757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	330.0
1	330.0
2	692.5
3	1055.0
4	1055.0
5	1055.0
6	2776.5
7	4498.0
8	4498.0
9	4498.0
10	4416.0
11	4334.0
12	4334.0
13	4334.0
14	4301.0
15	4268.0
16	4268.0
17	4268.0
18	6895.0
19	9522.0
20	9522.0
21	9522.0
22	15081.5
23	20641.0
24	20641.0
25	20641.0
26	30279.0
27	39917.0
28	39917.0
29	39917.0
30	49085.5
31	58254.0
32	58254.0
33	58254.0
34	70619.0
35	82984.0
36	82984.0
37	82984.0
38	92617.5
39	102251.0
40	102251.0
41	102251.0
42	118413.5
43	134576.0
44	134576.0
45	134576.0
46	162030.0
47	189484.0
48	189484.0
49	189484.0
50	182187.0
51	174890.0
52	174890.0
53	174890.0
54	158832.0
55	142774.0
56	142774.0
57	142774.0
58	130695.0
59	118616.0
60	118616.0
61	118616.0
62	106055.5
63	93495.0
64	93495.0
65	93495.0
66	77412.0
67	61329.0
68	61329.0
69	61329.0
70	46718.5
71	32108.0
72	32108.0
73	32108.0
74	25037.5
75	17967.0
76	17967.0
77	17967.0
78	14956.0
79	11945.0
80	11945.0
81	11945.0
82	8375.5
83	4806.0
84	4806.0
85	4806.0
86	3602.0
87	2398.0
88	2398.0
89	2398.0
90	1647.5
91	897.0
92	897.0
93	897.0
94	534.0
95	171.0
96	171.0
97	171.0
98	340.0
99	509.0
100	509.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008903980840459689
2	0.003652915216598847
3	0.005935987226973126
4	0.015144377668816053
5	0.03455048975699743
6	0.04596584980886882
7	0.06765503390742447
8	0.08896370600425108
9	0.09604122923641135
10	0.11628446772839662
11	0.1185675397387709
12	0.13196156219963334
13	0.12047009974741614
14	0.12282927415813623
15	0.11377308851698491
16	0.11133781170591901
17	0.12062230454810774
18	0.13005900219098812
19	0.13051561659306296
20	0.12001348534534129
21	0.12929797818753
22	0.14832357827398232
23	0.13667991102107352
24	0.13272258620309144
25	0.12983069498995067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1314019.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.043461018235455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.396584501526	40.23323342501389
2	11.572247213524346	11.58230604646779
3	3.293963389936836	4.945239854993963
4	1.3846653425956474	2.771737843819477
5	0.7419238533767498	1.8564218717479208
6	0.5072984700926846	1.5232182727616248
7	0.336490470164645	1.1787403408684494
8	0.25978476100012804	1.0400422848193216
9	0.18902920034679263	0.8513707876976643
>10	1.0788274665651258	10.095280459970745
>50	0.11132129935620173	3.9001721374018485
>100	0.11186198415770586	11.93875454742142
>500	0.011277633375551403	3.804399839059679
>1k	0.004419613079607982	3.4309992494392434
>5k	3.048009020419298E-4	0.8480830385170024
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5874	0.44702549963128385	No Hit
TCCATGTACTCTGCGTTGATACCAC	5246	0.3992331922141156	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3458	0.26316210039580856	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2810	0.21384774497172415	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2690	0.20471545693022705	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2581	0.19642029529253385	No Hit
CATGTACTCTGCGTTGATACCACTG	2130	0.16209811273657382	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1907	0.14512727745945836	No Hit
CCCATGTACTCTGCGTTGATACCAC	1589	0.120926714149491	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1499	0.11407749811836816	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1465	0.11149001650661064	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1438	0.10943525169727379	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1435	0.10920694449623636	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1429	0.1087503300941615	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1421	0.10814151089139502	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1400	0.10654336048413302	No Hit
GCGTTGATACCACTGCTTCCATGTA	1350	0.10273824046684256	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1335	0.10159670446165542	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1330	0.10121619245992639	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	7.61024003458093E-5
4	0.0	0.0	0.0	0.0	7.61024003458093E-5
5	0.0	0.0	0.0	0.0	1.522048006916186E-4
6	0.0	0.0	0.0	0.0	1.522048006916186E-4
7	0.0	0.0	0.0	0.0	1.522048006916186E-4
8	0.0	0.0	0.0	0.0	1.522048006916186E-4
9	0.0	0.0	0.0	0.0	1.522048006916186E-4
10	0.0	0.0	0.0	0.0	1.522048006916186E-4
11	0.0	0.0	0.0	0.0	1.522048006916186E-4
12	0.0	0.0	0.0	0.0	3.044096013832372E-4
13	0.0	0.0	0.0	0.0	3.8051200172904654E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCGTG	40	0.0052766264	14.250772	7
GTCTTAC	80	1.2942473E-7	14.244258	1
GCGTAAC	95	7.374365E-8	13.001199	11
CCGCATC	205	0.0	12.977303	12
GCGTTAT	110	2.761226E-9	12.949325	1
GCTCGTA	125	1.0186341E-10	12.921685	9
AATCCCG	75	1.4756091E-5	12.668801	19
CGAACGA	75	1.477345E-5	12.667353	16
CGACGGG	165	0.0	12.66687	14
CGCATCG	205	0.0	12.512396	13
CGAACCT	305	0.0	12.459691	16
CGTTATT	115	5.349648E-9	12.386784	2
CGCGTAA	100	1.4392936E-7	12.351609	10
TCGCGTA	100	1.4392936E-7	12.351609	9
TAGAGTG	185	0.0	12.322643	5
GTTATTC	140	5.2750693E-11	12.210759	3
TCTTATA	70	1.09437315E-4	12.209828	2
CCGTCAA	195	0.0	12.182003	18
CGTCAAT	195	0.0	12.181539	19
TCCGTCA	205	0.0	12.050812	17
>>END_MODULE