FastQCFastQC Report
Thu 2 Feb 2017
SRR4062556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314019
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA27970.21285841376722867No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24460.18614647124584957No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15260.116132262927705No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13890.10570623408032913No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13850.1054018244789459No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13440.1022816260647677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAACG250.00235295735.1997268
ACCGGTA551.5938052E-423.99981512
ATCGATT803.6161855E-621.99982829
CGGATCG755.4734275E-520.53317326
CGCAATA4950.020.44428636
CAATACG5350.019.32695238
GATATAC1851.8189894E-1219.0312231
CGTAAGA600.00740789118.3352812
CGTCGTA3600.018.33319310
ATAATAC600.007412770818.333193
TATACGG600.007412770818.333195
GCAATAC5550.018.23409337
AATACGA5550.018.23409339
ATACGAA5450.018.16499540
CGAATGC5400.017.92578743
CGGTCCA6250.017.59986310
GTAGTAC1254.853664E-717.5998633
TCGATTT902.2156871E-417.1109830
TGGTATA2700.017.11097744
ACCGTCG3750.017.01328
Produced by FastQC (version 0.11.5)