FastQCFastQC Report
Thu 2 Feb 2017
SRR4062555_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062555_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060994
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33200.16108732000190198No Hit
TATCAACGCAGAGTACTTTTTTTTT21350.10359079162772915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT456.758861E-414.77915813
CGCCAGT3850.013.32786318
GTTTTCG1650.012.66815715
TTAGTAC604.0998755E-412.6641573
TAGTACG1155.358743E-912.3864422
ATCGCCA4100.012.28223116
TTATACA1405.2750693E-1112.2130524
TCTTATA1251.4206307E-912.1552292
GCATCGC4100.011.81846314
CGCGCCA1459.822543E-1111.793344510
ACGGTAT2500.011.7802419
CGACCAT2350.011.72364410
AGAACCG2200.011.658485
CGCATCG4250.011.62461513
AATCGTA1157.068229E-811.56629713
TTAGAAC1157.096787E-811.5629263
CGTCGTA3050.011.52482610
TAGATCG1001.9316085E-611.3994025
TCGCCAG4450.011.31703917
TCGACGC1104.9630216E-711.22859414
Produced by FastQC (version 0.11.5)