##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062555_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2060994
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.220310199835613	32.0	32.0	32.0	32.0	32.0
2	30.86316796652489	32.0	32.0	32.0	32.0	32.0
3	30.836295011048065	32.0	32.0	32.0	32.0	32.0
4	30.92349468266283	32.0	32.0	32.0	32.0	32.0
5	30.703734702769633	32.0	32.0	32.0	32.0	32.0
6	34.35722714379566	36.0	36.0	36.0	32.0	36.0
7	34.24339614768408	36.0	36.0	36.0	32.0	36.0
8	34.254682449342404	36.0	36.0	36.0	32.0	36.0
9	34.413850307181875	36.0	36.0	36.0	32.0	36.0
10	34.07751696511489	36.0	36.0	36.0	32.0	36.0
11	34.40215983161523	36.0	36.0	36.0	32.0	36.0
12	34.16055117093985	36.0	36.0	36.0	32.0	36.0
13	34.28672572554797	36.0	36.0	36.0	32.0	36.0
14	34.19619659251798	36.0	36.0	36.0	32.0	36.0
15	34.116972684054396	36.0	36.0	36.0	32.0	36.0
16	34.13063162726335	36.0	36.0	36.0	32.0	36.0
17	34.062817261961946	36.0	36.0	36.0	32.0	36.0
18	34.07314092132243	36.0	36.0	36.0	32.0	36.0
19	34.05592301578753	36.0	36.0	36.0	32.0	36.0
20	34.058183575498035	36.0	36.0	36.0	32.0	36.0
21	34.04289192496436	36.0	36.0	36.0	32.0	36.0
22	33.9910159854905	36.0	36.0	36.0	32.0	36.0
23	33.954001321692346	36.0	36.0	36.0	32.0	36.0
24	33.92162713719691	36.0	36.0	36.0	32.0	36.0
25	33.46821339606035	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	86.0
5	203.0
6	365.0
7	76.0
8	320.0
9	342.0
10	235.0
11	87.0
12	156.0
13	135.0
14	308.0
15	367.0
16	580.0
17	796.0
18	1088.0
19	1574.0
20	2347.0
21	3550.0
22	5399.0
23	8066.0
24	11667.0
25	16574.0
26	23658.0
27	30568.0
28	42276.0
29	56597.0
30	73038.0
31	100925.0
32	146342.0
33	212243.0
34	475748.0
35	845266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.136207917236824	17.446016634155338	10.909492338001378	26.508283110606456
2	17.501586673110403	19.971653381312	36.10297557008631	26.423784375491287
3	19.078622713551844	23.472543213063464	28.434469911829485	29.01436416155521
4	12.986291371799407	15.311405545663964	35.71357512812562	35.98872795441101
5	14.939324647235475	36.15383159234395	33.45917707126448	15.447666689156097
6	34.098711446302495	35.34765318565581	16.74517860332896	13.808456764712734
7	30.2409420940754	30.42107573284288	20.758581110147592	18.579401062934128
8	28.316164486809157	32.161137864251174	19.64329074630577	19.879406902633896
9	27.596893426822582	14.212703419008065	18.662314368848012	39.528088785321344
10	16.26064166992546	26.124112536342594	31.38304668125633	26.23219911247562
11	37.44281678783006	21.120759382370302	22.247202473627834	19.189221356171803
12	24.633608470112218	23.912607269577197	28.369502426577153	23.08428183373343
13	29.325218584252216	19.307409275026064	25.23072917122101	26.136642969500706
14	23.48797825144916	19.743533439951495	24.71045978597343	32.05802852262592
15	25.383604257584647	26.865170657018833	21.996646316583053	25.754578768813463
16	26.117852215983056	25.656682923038066	23.252958206229234	24.972506654749647
17	24.35563354281073	25.4418360168981	24.976487231206303	25.22604320908487
18	25.106326331714833	24.65609735478937	25.93750334015778	24.30007297333801
19	25.823809033331322	24.882424046906003	25.094838124148545	24.198928795614126
20	25.68979865074087	24.419967713774792	24.697213406313352	25.193020229170987
21	26.738262272144276	24.065500019433326	24.167719305064324	25.02851840335808
22	25.97332421074729	24.3908827268239	24.46260646796534	25.17318659446347
23	24.856906577730093	24.08891519151682	25.151256306800075	25.902921923953016
24	24.809810466376124	25.082766092867104	24.841051204687567	25.266372236069206
25	24.88263230132028	24.50211949326499	25.059770924682077	25.555477280732653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	177.0
1	177.0
2	522.5
3	868.0
4	868.0
5	868.0
6	2428.0
7	3988.0
8	3988.0
9	3988.0
10	4381.0
11	4774.0
12	4774.0
13	4774.0
14	5772.5
15	6771.0
16	6771.0
17	6771.0
18	10863.0
19	14955.0
20	14955.0
21	14955.0
22	23390.0
23	31825.0
24	31825.0
25	31825.0
26	47232.5
27	62640.0
28	62640.0
29	62640.0
30	76153.0
31	89666.0
32	89666.0
33	89666.0
34	109738.5
35	129811.0
36	129811.0
37	129811.0
38	145933.5
39	162056.0
40	162056.0
41	162056.0
42	182852.0
43	203648.0
44	203648.0
45	203648.0
46	230577.0
47	257506.0
48	257506.0
49	257506.0
50	260993.0
51	264480.0
52	264480.0
53	264480.0
54	248251.0
55	232022.0
56	232022.0
57	232022.0
58	214197.5
59	196373.0
60	196373.0
61	196373.0
62	176106.5
63	155840.0
64	155840.0
65	155840.0
66	132638.5
67	109437.0
68	109437.0
69	109437.0
70	84659.5
71	59882.0
72	59882.0
73	59882.0
74	47930.0
75	35978.0
76	35978.0
77	35978.0
78	28591.5
79	21205.0
80	21205.0
81	21205.0
82	15474.0
83	9743.0
84	9743.0
85	9743.0
86	7173.5
87	4604.0
88	4604.0
89	4604.0
90	3104.0
91	1604.0
92	1604.0
93	1604.0
94	970.0
95	336.0
96	336.0
97	336.0
98	570.5
99	805.0
100	805.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008442528216967154
2	0.0037845816145025165
3	0.004900547988009669
4	0.015575008951991128
5	0.03721505254260808
6	0.049005479880096695
7	0.06851063127791736
8	0.08918026932635417
9	0.09728315560355828
10	0.1193113614110473
11	0.12028176695322741
12	0.1331881606642232
13	0.1222710983146967
14	0.12411486884483895
15	0.11460489453147364
16	0.11227592123024134
17	0.12314446330265881
18	0.13139291041118994
19	0.13343076204976823
20	0.12280482136289576
21	0.12974322098948374
22	0.15031581848370254
23	0.1406117630619012
24	0.13508045147147446
25	0.13192663345938901
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2060994.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.38293622755508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.71490567136934	41.47368850725094
2	11.63237686971359	11.954113577427604
3	3.2273282873351987	4.974888107205872
4	1.3011770475249063	2.6743318901492263
5	0.6995302063081577	1.7971957989990257
6	0.46154644159522756	1.4229366824725533
7	0.31115351661433427	1.1191586910821685
8	0.23662661367407534	0.972685616012586
9	0.1783138599595262	0.8246060725310568
>10	1.0084720594771464	9.515226276203563
>50	0.10420644603178975	3.750262177714946
>100	0.1058143734012181	11.470330853640878
>500	0.014479269746523716	5.0070837882954855
>1k	0.004069337249023005	3.0434919610141415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3320	0.16108732000190198	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2135	0.10359079162772915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.852027710900662E-5	0.0	0.0	0.0
3	0.0	9.704055421801324E-5	0.0	0.0	0.0
4	0.0	9.704055421801324E-5	0.0	0.0	0.0
5	0.0	9.704055421801324E-5	0.0	0.0	0.0
6	0.0	9.704055421801324E-5	0.0	4.852027710900662E-5	4.852027710900662E-5
7	0.0	9.704055421801324E-5	0.0	9.704055421801324E-5	4.852027710900662E-5
8	0.0	9.704055421801324E-5	0.0	9.704055421801324E-5	4.852027710900662E-5
9	0.0	9.704055421801324E-5	0.0	1.940811084360265E-4	4.852027710900662E-5
10	0.0	9.704055421801324E-5	0.0	1.940811084360265E-4	9.704055421801324E-5
11	0.0	9.704055421801324E-5	0.0	1.940811084360265E-4	1.4556083132701987E-4
12	4.852027710900662E-5	9.704055421801324E-5	0.0	1.940811084360265E-4	1.940811084360265E-4
13	4.852027710900662E-5	1.4556083132701987E-4	0.0	1.940811084360265E-4	2.4260138554503313E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTT	45	6.758861E-4	14.779158	13
CGCCAGT	385	0.0	13.327863	18
GTTTTCG	165	0.0	12.668157	15
TTAGTAC	60	4.0998755E-4	12.664157	3
TAGTACG	115	5.358743E-9	12.386442	2
ATCGCCA	410	0.0	12.282231	16
TTATACA	140	5.2750693E-11	12.213052	4
TCTTATA	125	1.4206307E-9	12.155229	2
GCATCGC	410	0.0	11.818463	14
CGCGCCA	145	9.822543E-11	11.7933445	10
ACGGTAT	250	0.0	11.780241	9
CGACCAT	235	0.0	11.723644	10
AGAACCG	220	0.0	11.65848	5
CGCATCG	425	0.0	11.624615	13
AATCGTA	115	7.068229E-8	11.566297	13
TTAGAAC	115	7.096787E-8	11.562926	3
CGTCGTA	305	0.0	11.524826	10
TAGATCG	100	1.9316085E-6	11.399402	5
TCGCCAG	445	0.0	11.317039	17
TCGACGC	110	4.9630216E-7	11.228594	14
>>END_MODULE